Metal ion type significantly affects the morphology but not the activity of lipase–metal–phosphate nanoflowers

Sharma, Nishat; Parhizkar, Marzieh; Cong, Weimin; Mateti, Srikanth; Kirkland, Mark; Puri, Munish; Sutti, Alessandra

doi:10.1039/c7ra00302a

Cited by 29 publications

(13 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many examples of metal‐phosphate nanoflowers synthesized via incubation have been reported in the literature ,,. In the present study also the method of incubation led to formation of good quality nanoflowers (Figure ) but we drifted towards the idea of reducing the time of nanoflowers synthesis.…”

Section: Resultsmentioning

confidence: 99%

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

2018

View full text Add to dashboard Cite

Herein we report an expeditious and facile sonochemical synthesis method that takes just a few minutes to produce organic‐inorganic hybrid nanoflowers and their application as a nanobiocatalyst. Surfactant treated Burkholderia cepacia lipase was used as the organic component and manganese sulfate as the inorganic component to synthesize the nanoflowers in less than 10 minutes. Careful investigation of the process parameters was performed to obtain the optimized value for each attribute viz. sonication time (7.5 min), lipase concentration (0.1 mg/mL), MnSO4 concentration (150 mM), sonication frequency (33 kHz) and pH (8.0). An additional post‐synthesis treatment of nanoflowers with N‐hydroxysuccinimide (NHS) increased their reusability to over nine cycles with an overall six‐fold increase in the activity. In addition, thermal stability was demonstrated through a twenty‐fold increase in the half‐life of immobilized lipase at 80 °C than in free form. The hybrid nanoflowers displayed a marked improvement in the kinetic resolution of racemic intermediates of metoprolol, a selective β‐1 blocker drug [C (%)=46.7, eep=96.5, E=156] and cloranolol, a non‐selective β‐blocker drug [C (%)=49.5, eep=96.0, E=157] compared to free lipase which signifies its importance as a nanobiocatalyst.

show abstract

Section: Resultsmentioning

confidence: 99%

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

2018

View full text Add to dashboard Cite

show abstract

“…Generally, the HNF are formed by coordination interaction between enzymes and metal ions [27,35]. Metal ions play a role in morphology and activity of the HNF [32,37]. Until now, the previous reports focused on single metal ion-protein HNF.…”

Section: Introductionmentioning

confidence: 99%

Bimetal based inorganic-carbonic anhydrase hybrid hydrogel membrane for CO2 capture

Wen

Zhang

et al. 2020

Journal of CO2 Utilization

View full text Add to dashboard Cite

In this study, we synthesized for the first time a bimetal-based inorganic-carbonic anhydrase (CA) hybrid nanoflower to immobilize CA using Cu 2+ and Zn 2+ instead of single metal ion. Subsequently, the synthesized bimetallic hybrid nanoflowers (CANF) were embedded into the poly(vinyl alcohol) (PVA)-chitosan (CS) hydrogel networks to obtain PVA/CS@CANF hydrogel membrane. The CANF exhibited a significantly higher activity recovery of 70% compared with 35% with CA/Zn3(PO4)2 hybrid nanoflowers and 10% with CA/Cu3(PO4)2 hybrid nanoflowers. The PVA/CS@CANF hydrogel membrane possessed excellent mechanical strength, high catalytic activity, and were easy to flow out without centrifugation or filtration. At the same time, the PVA/CS@CANF displayed higher thermostability, storage stability, and pH stability than free CA and CANF, and superior reusability and CO2 capture capacity. The hydrogel membrane maintained more than 75% of its original activity after 8 cycles.However, CANF only maintained 12% of its original activity. Furthermore, the amount of CaCO3 produced by PVA/CS@CANF membrane was 9.0-fold and 2.0-fold compared with free CA and CANF, respectively. Therefore, This approach to synthesizing bimetallic-based protein hybrid hydrogel membrane could have a bright future in CO2 capture.

show abstract

“…The enhanced activity of enzymes that is observed in various HNFs is mainly attributed to their high surface area, which decreases mass-transfer limitations, along with the specific interactions of the enzyme molecules and metal ions [8,22,23]. However, the biocatalytic activity and stability of some HNFs are reduced by the interactions between metal ions and proteins [24].…”

Section: Introductionmentioning

confidence: 99%

Development of Effective Lipase-Hybrid Nanoflowers Enriched with Carbon and Magnetic Nanomaterials for Biocatalytic Transformations

et al. 2019

View full text Add to dashboard Cite

In the present study, hybrid nanoflowers (HNFs) based on copper (II) or manganese (II) ions were prepared by a simple method and used as nanosupports for the development of effective nanobiocatalysts through the immobilization of lipase B from Pseudozyma antarctica. The hybrid nanobiocatalysts were characterized by various techniques including scanning electron microscopy (SEM), energy dispersion spectroscopy (EDS), X-ray diffraction (XRD), Raman spectroscopy, and Fourier transform infrared spectroscopy (FTIR). The effect of the addition of carbon-based nanomaterials, namely graphene oxide and carbon nanotubes, as well as magnetic nanoparticles such as maghemite, on the structure, catalytic activity, and operational stability of the hybrid nanobiocatalysts was also investigated. In all cases, the addition of nanomaterials during the preparation of HNFs increased the catalytic activity and the operational stability of the immobilized biocatalyst. Lipase-based magnetic nanoflowers were effectively applied for the synthesis of tyrosol esters in non-aqueous media, such as organic solvents, ionic liquids, and environmental friendly deep eutectic solvents. In such media, the immobilized lipase preserved almost 100% of its initial activity after eight successive catalytic cycles, indicating that these hybrid magnetic nanoflowers can be applied for the development of efficient nanobiocatalytic systems.

show abstract

Metal ion type significantly affects the morphology but not the activity of lipase–metal–phosphate nanoflowers

Abstract: Using different metal ions to prepare hybrid lipase nanoflowers resulted in a variety of different morphologies, but did not significantly affect the specific activity of the enzyme.

Cited by 29 publications

References 47 publications

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

Bimetal based inorganic-carbonic anhydrase hybrid hydrogel membrane for CO2 capture

Development of Effective Lipase-Hybrid Nanoflowers Enriched with Carbon and Magnetic Nanomaterials for Biocatalytic Transformations

Contact Info

Product

Resources

About