Metal enhanced fluorescence on nanoporous gold leaf-based assay platform for virus detection

Abstract

In the present study, a rapid, sensitive and quantitative detection of influenza A virus targeting hemagglutinin (HA) was developed using hybrid structure of quantum dots (QDs) and nanoporous gold leaf (NPGL). NPGL film was prepared by dealloying bimetallic film where its surface morphology and roughness were fairly controlled. Anti-influenza A virus HA antibody (ab66189) was bound with NPGL and amine (-NH2) terminated QDs. These biofunctionalized NPGL and QDs formed a complex with the influenza virus A/Beijing/262/95 (H1N1) and the photoluminescence (PL) intensities of QDs were linearly correlated with the concentrations of the virus up to 1ng/mL while no PL was observed in the absence of the virus, or in bovine serum albumin (BSA, 1µg/mL) alone. In addition, it was demonstrated that this assay detected successfully influenza virus A/Yokohama/110/2009 (H3N2) that is isolated from a clinical sample, at a concentration of ca. 50 plaque forming units (PFU)/mL. This detection limit is 2-order more sensitive than a commercially available rapid influenza diagnostic test. From these results, the proposed assay may offer a new strategy to monitor influenza virus for public health.

Detection of influenza virus using peroxidase‐mimic of gold nanoparticles

“…The importance of developing simple, sensitive, highly selective, and cost‐effective bioassays for disease‐related protein detection to control the spread of infectious viruses is increasing (Gao et al, ; Gubala et al, ; Rodríguez‐Lorenzo et al, ). To date, various immunoassays for disease‐related protein detection have been reported and developed, for example, surface plasmon resonance (Nguyen et al, ), metal‐enhanced fluorescence (Ahmed et al, ), chemilluminescence (Khavan‐Tafti et al, ), electrochemistry (Munge et al, ) and colorimetric methods (Gao et al, ). In particular, the enzymatic colorimetric method is used widely in immunoassays because of its simplicity, low cost, and easy readout (Gao et al, ; Perfezou et al, ).…”

In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform

“…The sensitivity of detection in this study was compared with that of a commercially available rapid influenza diagnostic kit, which we have studied previously1 and the conventional ELISA method on Au NPs film (Table 1 and Fig. 7C).…”

“…This sample was immersed in synthesized thioglycolic acid (TGA)-capped cadmium telluride (CdTe) quantum dots (QDs) solution48 for 10 min, at this time the final concentration of the QD solutions was adjusted to the nanomolar level to prevent aggregation with DI water. The polymer spacer layer between nanocrystals and metal surface avoids unwanted quenching effects due to radioactive energy transfer from nanocrystals to metal surface and assists in photo-luminescence (PL) enhancement1.…”

“…Such nanostructured films are increasingly used in a broad range of applications, including energy storage1234567, electronics89101112131415, and biomedicine1617. In particular, noble-metal NP films have been extensively studied because of their versatility, chemical stability, and unique optical properties stemming from localized surface plasmon resonances, which lead to enormous local, nanoscale optical field enhancements.…”

“…SERS and immunosensing applications were demonstrated films fabricated via the proposed method. The Au NP films were further applied to develop a colorimetric sensor to detect influenza virus A as a model analyte because of the high demand for sensitive and accurate diagnosis to prevent infection at early stages1.…”