In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform

Abstract

Nanomaterials without chemical linkers or physical interactions that reside on a two-dimensional surface are attractive because of their electronic, optical and catalytic properties. An in situ method has been developed to fabricate gold nanoparticle (Au NP) films on different substrates, regardless of whether they are hydrophilic or hydrophobic surfaces, including glass, 96-well polystyrene plates, and polydimethylsiloxane (PDMS). A mixture of sodium formate (HCOONa) and chloroauric acid (HAuCl4) solution was used to prepare Au NP films at room temperature. An experimental study of the mechanism revealed that film formation is dependent on surface wettability and inter particle attraction. The as-fabricated Au NP films were further applied to the colorimetric detection of influenza virus. The response to the commercial target, New Caledonia/H1N1/1999 influenza virus, was linear in the range from 10 pg/ml to 10 μg/ml and limit of detection was 50.5 pg/ml. In the presence of clinically isolated influenza A virus (H3N2), the optical density of developed color was dependent on the virus concentration (10–50,000 PFU/ml). The limit of detection of this study was 24.3 PFU/ml, a limit 116 times lower than that of conventional ELISA (2824.3 PFU/ml). The sensitivity was also 500 times greater than that of commercial immunochromatography kits.

Compartmentalized Thin Films with Customized Functionality via Interfacial Cross‐linking of Protein Cages

“…Such a local acidic environment can promote the hydrolysis of NaBH 4 and thus enhance the reduction reaction . These results do clearly indicate that the catalytic activity of Au NPs can be maintained in the compartments within the films created by cross‐linking the protein cages, which allows for further applications in, e.g., catalytic or sensor devices …”

Chiral MoS2 Quantum Dots: Dual‐Mode Detection Approaches for Avian Influenza Viruses

“…The limit of detection (LOD) was calculated based on the standard deviation method as reported previously …”

Detection methods for influenza A H1N1 virus with special reference to biosensors: a review

“…More the number of antibodies binds with nanoparticles, and more antigens can be captured that help in increasing the sensitivity of the procedure. The catalytic activity of gold nanoparticles toward TMB-H 2 O 2 makes it oxidized, which further causes a change in colour and gives a signal [54].…”