Metal Enhanced Electrochemical Cyclooxygenase‐2 (COX‐2) Sensor for Biological Applications

Noah, Naumih M.; Marcells, Omole; Almalleti, Anas; Lim, Jae; Sadik, Omowunmi A.

doi:10.1002/elan.201100241

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…24 The peak currents of the anodic and cathodic peaks indicated insignificant changes after several cycles and this indicated the stability as well as the reproducibility of the AuNPs-rabbit anti-bilharzia conjugate. After the incubation of the conjugate modified electrode with different concentrations of standards of SEA prepared in PBS buffer (pH 7.4), the results indicated a biospecific molecular recognition, forming an antibody-antigen complex evidenced by the change in the redox current 25 as shown in Fig. 3a.…”

Section: Cyclic Voltammetry (Cv)mentioning

confidence: 92%

Development of an electrochemical nano-biosensor for rapid and sensitive diagnosis of bilharzia in Kenya

Odundo¹,

Noah²,

Andala³

et al. 2018

S.Afr.j.chem.

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Bilharzia, also known as schistosomiasis, is still affecting more than 250 million people, killing about 200 000 annually and leaving millions of others debilitated. Current diagnostic tools have extremely low validity and are not readily applicable for rapid diagnosis in rural and remote areas. In low-grade infections, microscopy, the most commonly used method, may have low sensitivity. For example, in suspected cases, up to five real sample specimens are recommended for increased sensitivity. The rise of nanotechnology has created the potential to significantly improve the current diagnostic approaches. Conjugating antibodies with nanoparticles pools the distinctive properties of the nanoparticles with the specificity and selectivity of the antibodies in the recognition of the antigen. This in return leads to a very versatile and specific mixed product with improved analytical signal which is important for fast, sensitive and real-time point-of-care diagnosis. In this work, we report the use of nanotechnology to develop a simple and sensitive nano-biosensor which consisted of a nano-strip with immobilized gold nanoparticles conjugated with bilharzia antibody, and demonstrated its potential for diagnosis of bilharzia antigen. A detection range of 1.13 × 10 1 ng mL-1 to 2.3 × 10 3 ng mL-1 of bilharzia antigen in stool samples and with a limit of detection of 8.3887 × 10-2 ng mL-1 was attained, indicating the potential of the nano-biosensor to be used to detect bilharzia antigen in stool samples.

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Section: Cyclic Voltammetry (Cv)mentioning

confidence: 92%

Development of an electrochemical nano-biosensor for rapid and sensitive diagnosis of bilharzia in Kenya

Odundo¹,

Noah²,

Andala³

et al. 2018

S.Afr.j.chem.

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“…Our group conducted extensive research in this area, identifying COX-2 and iNOS or nitric oxide synthase 2 as good candidates for this purpose [58,60,62,63,65,[68][69][70][71]. COX, also known as prostaglandin H 2 synthase, is a key bifunctional enzyme in the biosynthetic pathway that leads to the formation of prostanoids, including prostaglandins, prostacyclins, and thromboxanes.…”

Section: Biomarkers or Biosensorsmentioning

confidence: 99%

A Bayesian Network Concept for Pain Assessment

Sadik¹,

Schaffer²,

Land³

et al. 2022

JMIR Biomed Eng

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In this study, we propose an approach that provides a useful data summary related to a patient’s experience of pain. Because pain is a very important but subjective phenomenon that currently has no calibratable method for assessing it, we suggest an approach that uses calibratable biomarker sensors with the patient’s self-assessment of perceived pain. We surmise that such an approach may only be able to clearly distinguish between cases in which the available evidence is consistent. However, this information may provide clinicians with valuable insights, and as research progresses into how biomarkers are related to pain, more specific insights may emerge regarding how specific evidence inconsistencies may point to particular pain causes. We provide a brief overview of pain science, including the types of pain, contemporary pain theories, pain, and pain assessment techniques. Next, we present novel approaches to pain sensor development, including an overview of research on pain-related biomarker sensors and artificial intelligence methods for summarizing the evidence. We then provide some illustrations of the implementation of our approach. Some specifics are presented in the Methods section of this paper. For example, in a set of 379 patients, we observed 80% evidence of consistency and 5 types of inconsistencies. Information regarding the gender and individual differences in cyclooxygenase-2 and inducible nitric oxide synthase data on reported pain could contribute to the inconsistency. Different causes of inconsistencies are also attributed to cultural or temporal variability of cyclooxygenase-2 and inducible nitric oxide synthase (as well as their serum variation and half-life), visual analog scale, and other tools. We emphasize that this presentation is illustrative. Much work remains to be done before implementing and testing this approach in a clinically meaningful context.

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“…Several traditional methods, for example, electrochemical analysis, immunohistochemical method, positron emission tomography , and single-photon emission computed tomography, , have been proposed for the detecting and imaging of COX-2 at trace quantity levels in various samples. These methods show high selectivity toward COX-2 and could meet the demands for the detection of COX-2 in solution and biological fluids.…”

mentioning

confidence: 99%

Highly Sensitive Naphthalene-Based Two-Photon Fluorescent Probe for in Situ Real-Time Bioimaging of Ultratrace Cyclooxygenase-2 in Living Biosystems

et al. 2014

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Detecting and imaging of ultratrace cyclooxygenase-2 in living biosystems could provide much important valuable information for the diagnosis and intervention of cancer. Molecular probes, whose fluorescent signals are generated by cyclooxygenase-2, hold great potential for identification and enumeration of cyclooxygenase-2 in living biosystems. Although quite a few fluorescent probes have been reported for cyclooxygenase-2, the use fluorogenic probe with the excellent two-photon properties for the determination of ultratrace cyclooxygenase-2 has been scarce. Herein, an "off-on" fluorescence probe (BTDAN-COX-2), able to report and image the presence of ultratrace cyclooxygenase-2 in living biosystems, has been designed and evaluated. In order to improve sensitivity and specific selectivity of probe for ultratrace cyclooxygenase-2, BTDAN-COX-2 employed cyclooxygenase-2's inhibitor as recognition group, because it is a classical and efficient recognition group for cyclooxygenase-2. A polarity-sensitive naphthalene derivative (BTDAN) as fluorophore was introduced into the molecule to enhance two-photon properties of BTDAN-COX-2. In the absent of cyclooxygenase-2, BTDAN-COX-2 mainly exists in a folded conformation where probe fluorescence is quenched through photoinduced electron transfer between the fluorophore and the recognition group. Under the condition of existence of cyclooxygenase-2, fluorescence of probe is turned on, because photoinduced electron transfer between the fluorophore and the recognition group is restrained. BTDAN-COX-2 provides high signal-to-background staining for the ultratrace cyclooxygenase-2 and has been successfully used to rapidly detect and image ultratrace cyclooxygenase-2 in living biosystems.

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Metal Enhanced Electrochemical Cyclooxygenase‐2 (COX‐2) Sensor for Biological Applications

Cited by 7 publications

References 20 publications

Development of an electrochemical nano-biosensor for rapid and sensitive diagnosis of bilharzia in Kenya

Development of an electrochemical nano-biosensor for rapid and sensitive diagnosis of bilharzia in Kenya

A Bayesian Network Concept for Pain Assessment

Highly Sensitive Naphthalene-Based Two-Photon Fluorescent Probe for in Situ Real-Time Bioimaging of Ultratrace Cyclooxygenase-2 in Living Biosystems

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