Metal binding characterization of heterologously expressed metallothionein of the freshwater crab Sinopotamon henanense

Yang, Hui Zhen; Gu, Wen J.; Chen, Wei; Hwang, Jiang Shiou; Wang, Lan

doi:10.1016/j.chemosphere.2019.06.097

Cited by 24 publications

(2 citation statements)

References 31 publications

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“…This is in agreement with the literature. 33,34 Aer the whole washing process, the signal of Zn and Cd decreased approximately to 4% of its original value. Based on the recalculation, it was determined that the MT amount remaining trapped within the polymeric layer is 0.7 mM.…”

Section: Resultsmentioning

confidence: 93%

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Veverkova

Pavelicová

Vlčnovská

et al. 2023

J. Anal. At. Spectrom.

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Section: Resultsmentioning

confidence: 93%

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Veverkova

Pavelicová

Vlčnovská

et al. 2023

J. Anal. At. Spectrom.

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“…Metallothionein structure can be determined using 113 Cdand 1 H-nuclear magnetic resonance (Duncan et al, 2008), electron paramagnetic resonance (Krepkiy et al, 2003), Raman spectroscopy (Giner et al, 2018), circular dichroism (Yang et al, 2019), Mossbauer spectroscopy (Afonso et al, 2004), and X-ray absorption near-edge structure spectroscopy (Manceau et al, 2019). Mass spectrometry (MS) can be coupled with electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) (Ryvolova et al, 2011).…”

Section: Current Analytical Techniques For the Determination Of Mts And Pcsmentioning

confidence: 99%

Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation

et al. 2020

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The persistence of heavy metals (HMs) in the environment causes adverse effects to all living organisms; HMs accumulate along the food chain affecting different levels of biological organizations, from cells to tissues. HMs enter cells through transporter proteins and can bind to enzymes and nucleic acids interfering with their functioning. Strategies used by microalgae to minimize HM toxicity include the biosynthesis of metal-binding peptides that chelate metal cations inhibiting their activity. Metalbinding peptides include genetically encoded metallothioneins (MTs) and enzymatically produced phytochelatins (PCs). A number of techniques, including genetic engineering, focus on increasing the biosynthesis of MTs and PCs in microalgae. The present review reports the current knowledge on microalgal MTs and PCs and describes the state of art of their use for HM bioremediation and other putative biotechnological applications, also emphasizing on techniques aimed at increasing the cellular concentrations of MTs and PCs. In spite of the broad metabolic and chemical diversity of microalgae that are currently receiving increasing attention by biotechnological research, knowledge on MTs and PCs from these organisms is still limited to date.

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Study on metal binding capacity of the freshwater crab Sinopotamon henanense’s recombinant copper specific binding metallothionein expressed in Escherichia coli

et al. 2021

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Metal binding characterization of heterologously expressed metallothionein of the freshwater crab Sinopotamon henanense

Cited by 24 publications

References 31 publications

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation

Study on metal binding capacity of the freshwater crab Sinopotamon henanense’s recombinant copper specific binding metallothionein expressed in Escherichia coli

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