Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Abstract

Effective detection and sensitive quantification of disease markers enable a better understanding of processes leading to disease development. Therefore, we present an approach that combines the excellent selective recognition capabilities...

Atomic spectrometry update: review of advances in the analysis of clinical and biological materials, foods and beverages

“…183 Specificity was confirmed using anti-metallothionein antibody labelled Ag NPs as tags. 183 In a different approach, lanthanide labelled antibodies were employed for multiplexed quantitation of MMP-11 and CD45 in immunohistochemistry-assisted LA-ICP-MS. 201 Analysis of paraffin embedded breast cancer ( n = 21) and healthy breast tissue samples ( n = 4) showed overexpression of MMP-11 in metastatic tissue compared with other tissue types ( P < 0.01) but no significant differences in the expression of CD45. 201 These results support previous findings relating to the potential of MMP-11 as a prognostic biomarker.…”

“…The work provided useful data on the impact of Pt-drug distribution for this aggressive disease. Veverkova and co-workers 183 published an investigation on the use of magnetic particles with molecular imprinted polymers for the detection of metallothionein as a marker for melanoma. The molecular imprinted polymers are an alternative to antibody labelling and in combination with magnetic particles provided specific isolation of the analyte from the tissue matrix, in this case metallothionein.…”

“…200 Veverkova et al reported adaptations to their previously described sandwich immunoassay for metallothionein with LA-ICP-MS readout. 183 Magnetic particles were modified by molecularly imprinted polymers and incubated with sample and spotted onto PVDF for LA-ICP-MS analysis. 183 Specificity was confirmed using anti-metallothionein antibody labelled Ag NPs as tags.…”

“…183 Magnetic particles were modified by molecularly imprinted polymers and incubated with sample and spotted onto PVDF for LA-ICP-MS analysis. 183 Specificity was confirmed using anti-metallothionein antibody labelled Ag NPs as tags. 183 In a different approach, lanthanide labelled antibodies were employed for multiplexed quantitation of MMP-11 and CD45 in immunohistochemistry-assisted LA-ICP-MS. 201 Analysis of paraffin embedded breast cancer ( n = 21) and healthy breast tissue samples ( n = 4) showed overexpression of MMP-11 in metastatic tissue compared with other tissue types ( P < 0.01) but no significant differences in the expression of CD45.…”