Metageographic population analysis of Colletotrichum truncatum associated with chili fruit rot and other hosts using ITS region nucleotide sequences

Abstract

Colletotrichum truncatum is one of the most economically important fungal pathogen causing anthracnose disease in pre and post-harvest stages of many crops worldwide. Little information is available in the literature on the genetic analysis and demographic history of this fungal pathogen. In the present study nucleotide sequence data of internal transcribed spacer (ITS) region were analyzed for C. truncatum isolates infecting chili and other crops worldwide to determine a metageographic pattern of distribution and evolution of the species. Levels of differentiation (genetic distances and F ST values) among sequences of C. truncatum from 23 countries were minimal suggesting the global occurrence of a large and geographically undifferentiated population. Only 11 haplotypes were detected among 98 isolates from 24 geographically distant populations of C. truncatum. Predominant haplotype H1 which occupied a central position in the median joining network was inferred to be ancestral haplotype as it was detected at a high frequency and was shared by multiple populations. Phylogeographic pattern of the species with worldwide presence and predominance of single haplotype suggests human mediated dispersal through domestication and introduction of host plants in different parts of the world, and might have played a significant role in structuring the populations of this devastating pathogen.

Phylogeny and variability ofColletotrichum truncatumassociated with soybean anthracnose in Brazil

“…Because of the obvious implications of this epidemiological hypothesis, further studies should be conducted to elucidate this aspect. Information on the levels of genetic diversity among C. truncatum populations from distinct hosts and countries could contribute to elaborate effective management strategies based on host resistance (Katoch et al., ).…”

Characterization of Colletotrichum truncatum from papaya, pepper and physic nut based on phylogeny, morphology and pathogenicity

“…Of these, comparison of multiple nucleotide sequences, such as the nuclear rDNA internal transcribed spacer (ITS) region, b-tubulin (TUB2), actin (ACT), glutamine synthetase (GS) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), have provided substantial information regarding the phylogenetic relationship among individuals and the genetic structure of the population (Rampersad et al, 2013). Previous reports have suggested the usefulness of a polyphasic approach to determine the greatest variability among closely related Colletotrichum species (Cai et al, 2009) and even among individuals of the same species (Katoch et al, 2016).…”

Molecular Diversity, Haplotype Distribution and Gene Flow of Bipolaris Sorokiniana Fungus Causing Spot Blotch Disease in Different Wheat Growing Zones

“…Thus, domestication, introduction, and continuous cultivation of host plants of B. sorokiniana could be the plausible explanation for topical population expansion in different wheat growing zones of India. Such type of fungal population movement has been documented in different types of fungal pathogens (Kashyap et al 2020b;Dietzel et al 2019;Katoch et al 2016;Linde et al 2010;Zaffarano et al 2008).…”

“…A large number of studies have revealed the signi cance of internal transcribed spacer (ITS) region (a nuclear rDNA repeat unit) in resolving their fungal taxonomic status at the genus and species level (Kashyap et al 2017;Zhang et al 2017;Kashyap et al 2016;Katoch et al 2016;Rai et al 2016;Nilsson et al 2009;Wickert et al 2012). Glass and Donaldson (1995) reported high rate of evolution in ITS1 and ITS2 spacers and provided strong evidence for their deployment as an evolutionary marker for dissecting inter-and intra-speci c variations.…”