Characterization of Colletotrichum truncatum from papaya, pepper and physic nut based on phylogeny, morphology and pathogenicity

Abstract

Colletotrichum truncatum (syn. C. capsici) has been identified as the causal agent of anthracnose on various hosts, predominantly pepper (Capsicum spp.) plants. The aim of this study was to determine whether C. truncatum isolates infecting papaya, pepper and physic nut in southeastern Mexico are morphologically, genetically and pathogenically different, in order to improve disease management strategies. A total of 113 C. truncatum isolates collected from five producer states were subjected to phenotypic characterization and divided into six different morphological groups. These morphological traits and the location of the isolates were used to select a subset of 20 isolates for further studies. Differences in the pathogenicity of the isolates were tested with a cross‐inoculation assay using pepper, papaya and physic nut. The pathogenicity tests revealed that all isolates could infect the three hosts and produce typical anthracnose symptoms, indicating a lack of host specificity for this species and therefore its pathogenic potential on other plants. Phylogenetic analysis using internal transcribed spacer (ITS) and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) sequences of the C. truncatum isolates from this study and reference strains was performed, grouping the isolates into a monophyletic clade. This study reports for the first time the characterization of C. truncatum causing anthracnose disease on three different hosts in Mexico.

The Role of Glyceraldehyde-3-Phosphate Dehydrogenases in NADPH Supply in the Oleaginous Filamentous Fungus Mortierella alpina

“…The NADH produced from the reaction catalyzed by GAPDH can be transformed into NADPH in the cytoplasm through the pyruvate-oxaloacetate-malate cycle (POM cycle). The gapdh is traditionally used as a conserved gene for species identification (Ghuffar et al, 2018;Huang et al, 2018;Torres-Calzada et al, 2018). Its promoter is highly effective for heterologous protein expression in microorganisms (Madhavan et al, 2017;Prielhofer et al, 2017;Liu et al, 2018).…”

Causal agent of anthracnose in the pea crop (Pisum sativumL.) in northern of Perú: Symptomatology, isolation and identification, pathogenicity and control

“…La antracnosis es una enfermedad destructiva y responsable de pérdidas significativas de rendimiento en pimiento, tomate, papaya, mango, leguminosas y frutales (Liu et al, 2016;He et al, 2016;Torres-Calzada et al, 2018;Almaraz et al, 2019). Además, los tallos y vainas infectadas de frijol por C. lindemuthianum pueden sobrevivir de 9 a 18 meses en el suelo, por lo que es importante eliminar los restos de tejido infectado y realizar rotación de cultivos con gramíneas (Conner et al, 2019).…”

Geographical distribution, diversity and pathogenicity of Colletotrichum associ-ated with soybean anthracnose in Brazil

“…This pattern was also reported by Hartman et al (2015). Usually, Colletotrichum truncatum produce whitish colonies that eventually turn smoky black and, for that reason, isolates of C. truncatum greatly vary the colony characteristics and pathogenicity (Torres-Calzada et al, 2017;Majonjo & Kapooria, 2003).…”