Metagenomic analysis reveals that activated carbon aids anaerobic digestion of raw incineration leachate by promoting direct interspecies electron transfer

Lei, Yuqing; Sun, Dezhi; Dang, Yan; Feng, Xianlu; Huo, Da; Liu, Chuanqi; Zheng, Kai; Holmes, Dawn E.

doi:10.1016/j.watres.2019.06.038

Cited by 119 publications

(23 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the category of metabolism, the most abundant metabolic type was carbohydrate metabolism (4.28% in the PSD and 4.82% in the AnMBR), followed by amino acid metabolism (4.19% in the PSD and 4.73% in the AnMBR) as well as energy metabolism (3.15% in the PSD and 3.35% in the AnMBR) ( Figure S2). A high proportion of genes related to these functions has been previously reported in metagenomes [49][50][51], metatranscriptomes [52] and metaproteomes [53] from AD systems, indicating these metabolic activities are linked to the conversion of excess carbohydrates into methane during anaerobic digestion. When classified as KEGG level 3, the abundance of genes in methane metabolism was one of the categories with the highest abundance in the PSD and AnMBR, consisting of 1.05% and 0.88% of the total sequences (Table S5), respectively, suggesting methanogenesis was the major microbial metabolism in these two bioreactors.…”

Section: Variation Of Gene Functional Profiles Of the Psd And Anmbrmentioning

confidence: 84%

Genome-Centered Metagenomics Analysis Reveals the Microbial Interactions of a Syntrophic Consortium during Methane Generation in a Decentralized Wastewater Treatment System

Zhang

Xinping

et al. 2019

Applied Sciences

View full text Add to dashboard Cite

The application of anaerobic digestors to decentralized wastewater treatment systems (DWTS) has gained momentum worldwide due to their ease of operation, high efficiency, and ability to recycle wastewater. However, the microbial mechanisms responsible for the high efficiency and ability of DWTS to recycle wastewater are still unclear. In this study, the microbial community structure and function of two different anaerobic bioreactors (a primary sludge digestor, PSD, and anaerobic membrane bioreactor, AnMBR) of a DWTS located in Germany was investigated using 16S rRNA gene amplicon and metagenomic sequencing, respectively. The results showed that the microbial community structure was remarkably different in PSD and AnMBR. Methanobacteriaceae and Syntrophaceae were identified as the families that significantly differed in abundance between these two bioreactors. We also used genome-centered metagenomics to predict the microbial interactions and methane-generating pathway, which yielded 21 near-complete assembled genomes (MAGs) (average completeness of 93.0% and contamination of 2.9%). These MAGs together represented the majority of the microbial community. MAGs affiliated with methanogenic archaea, including Methanobacterium sp., Methanomicrobiales archaea, Methanomassiliicoccales archaea, and Methanosaeta concilii, were recruited, along with other syntrophic bacterial MAGs associated with anaerobic digestion. Key genes encoding enzymes involved in specific carbohydrate-active and methanogenic pathways in MAGs were identified to illustrate the microbial functions and interactions that occur during anaerobic digestion in the wastewater treatment. From the MAG information, it was predicted that bacteria affiliated with Bacteroidetes, Prolixibacteraceae, and Synergistaceae were the key bacteria involved in anaerobic digestion. In the methane production step, Methanobacterium sp. performed hydrogenotrophic methanogenesis, which reduced carbon dioxide to methane with hydrogen as the primary electron donor. Taken together, our findings provide a clear understanding of the methane-generating pathways and highlight the syntrophic interactions that occur during anaerobic digestion in DWTS.

show abstract

Section: Variation Of Gene Functional Profiles Of the Psd And Anmbrmentioning

confidence: 84%

Genome-Centered Metagenomics Analysis Reveals the Microbial Interactions of a Syntrophic Consortium during Methane Generation in a Decentralized Wastewater Treatment System

Zhang

Xinping

et al. 2019

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…In AD, a metagenomic method can provide insight into the progress of a digester. An example is the ability to follow the AD process from the initial step, through an acidic condition (i.e., where volatile fatty acids (VFAs) are accumulated), and back to its normal operation condition [73,74]. The main objective of metagenomic methods, especially in a less complex environment, is to rebuild large fragments of genomes (or complete genomes) from species present in the microbial community [75,76].…”

Section: Determination Of Metabolic Functionality By Meta-omic Techniques 41 Metagenomicsmentioning

confidence: 99%

“…Therefore, the balance in production and consumption of these electron transfer mediators has a significant effect on the stability of AD. Lie et al [74] added granular activated carbon (GAC) into upflow anaerobic sludge bed (UASB) reactors (i.e., fed by municipal wastewater) in order to assess the increase in the electron transfer among syntrophic bacteria and methanogens. The study indicated that the GAC can enhance methane production through enhanced electron transfer.…”

Section: Metagenomicsmentioning

confidence: 99%

Molecular Microbial Community Analysis as an Analysis Tool for Optimal Biogas Production

Hashemi

Lien

et al. 2021

Microorganisms

View full text Add to dashboard Cite

The microbial diversity in anaerobic digestion (AD) is important because it affects process robustness. High-throughput sequencing offers high-resolution data regarding the microbial diversity and robustness of biological systems including AD; however, to understand the dynamics of microbial processes, knowing the microbial diversity is not adequate alone. Advanced meta-omic techniques have been established to determine the activity and interactions among organisms in biological processes like AD. Results of these methods can be used to identify biomarkers for AD states. This can aid a better understanding of system dynamics and be applied to producing comprehensive models for AD. The paper provides valuable knowledge regarding the possibility of integration of molecular methods in AD. Although meta-genomic methods are not suitable for on-line use due to long operating time and high costs, they provide extensive insight into the microbial phylogeny in AD. Meta-proteomics can also be explored in the demonstration projects for failure prediction. However, for these methods to be fully realised in AD, a biomarker database needs to be developed.

show abstract

“…can be demonstrated by an observed remarkable change in the expression of genes encoding certain enzymes, and cofactors, e.g., F 420 , ,, Pil A, and omcS . , F 420 cofactor is generated from the oxidation of F 420 H 2 , coupled with methanophenazine reduction, by membrane-bound Fpo complex. ,, The fundamental role of the F 420 cofactor is to transport electrons required for the reduction of CO 2 (Figure ). The electron donation by F 420 is expected to increase the interaction of NMDs with microorganisms. , Zhuang et al showed that increased conversion of acetate to CH 4 correlated with the concentration of the cofactor F 420 when Fe 3 O 4 was loaded on the water hyacinth-derived biochars. Furthermore, the low expression of Pil A, and omcS was considered as a clue for the interaction between NMDs and microbes.…”

Section: Extracellular Electron Transfer Via a Biotic–abiotic Relatio...mentioning

confidence: 99%

Perspectives on Potential Applications of Nanometal Derivatives in Gaseous Bioenergy Pathways: Mechanisms, Life Cycle, and Toxicity

Elsamadony

Elreedy

Mostafa

et al. 2021

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Nanosized metal derivatives (NMDs), referring to metals and their oxides, are extensively utilized as additives for anaerobic digestion (AD) and dark fermentation (DF) processes, for enhancing the production of methane (CH 4 ) and hydrogen (H 2 ), respectively. NMDs-derived positive impacts were widely confirmed in many previous studies; however, no consensus exists about how these have been acquired. Undoubtedly, NMDs affect extracellular electron transfer (EET). Consequently, we explore how biotic−biotic interactions, referring to direct interspecies electron transfer (DIET) among AD partners, and biotic−abiotic exchanges, which are mediated by redox reactions with metals, are affected. In this perspective, the mechanisms behind all those effects are reviewed and explained in detail, considering the specific properties of each NMD, e.g., size and type. We discuss previous studies that offer contradicting interpretations about which process dominates metal oxidation, metal reduction, or DIET. In addition, the fate of NMDs residues in the digestate after the treatment process is discussed, focusing on NMDs toxicity. From previous literature, the environmental impacts are evaluated for the production process of NMDs that are utilized in AD and DF processes via life-cycle assessment. This review provides a comprehensive understanding of NMDs−microbes interactions, which are mandatory for (i) building clear scientific knowledge about processes in play and (ii) engineering favorable conditions to achieve optimum yields in AD and DF processes.

show abstract

Metagenomic analysis reveals that activated carbon aids anaerobic digestion of raw incineration leachate by promoting direct interspecies electron transfer

Cited by 119 publications

References 46 publications

Genome-Centered Metagenomics Analysis Reveals the Microbial Interactions of a Syntrophic Consortium during Methane Generation in a Decentralized Wastewater Treatment System

Genome-Centered Metagenomics Analysis Reveals the Microbial Interactions of a Syntrophic Consortium during Methane Generation in a Decentralized Wastewater Treatment System

Molecular Microbial Community Analysis as an Analysis Tool for Optimal Biogas Production

Perspectives on Potential Applications of Nanometal Derivatives in Gaseous Bioenergy Pathways: Mechanisms, Life Cycle, and Toxicity

Contact Info

Product

Resources

About