Metagenomic analysis of bacterial community composition and antibiotic resistance genes in a wastewater treatment plant and its receiving surface water

Tang, Junying; Bu, Yuanqing; Zhang, Xuxiang; Huang, Kailong; He, Xiwei; Ye, Lin; Shan, Zhengjun; Ren, Hongqiang

doi:10.1016/j.ecoenv.2016.06.016

Cited by 132 publications

(46 citation statements)

References 64 publications

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“…In this manner, a profile of the types and numbers of ARGs detected in a sample can be obtained and compared with other samples using various bioinformatics techniques and graphical representations. Metagenomics has been successfully applied in this manner for monitoring ARGs in wastewater treatment plants (Schluter et al, 2008;Wang et al, 2013;Yang et al, 2014;Li et al, 2015a;Munck et al, 2015;Zhang et al, 2015a;Bengtsson-Palme et al, 2016;Hu et al, 2016;Karkman et al, 2016;Ma et al, 2016b), biosolids (McCall et al, 2016;Tao et al, 2016;Rowe et al, 2016;Tang et al, 2016), manure (Agga et al, 2015), soil (Yan et al, 2016), rivers (Garner et al, 2016;Rowe et al, 2016), sediments (Cummings et al, 2011), and (Port et al, 2012). However, next-generation DNA sequencing technologies are still costly and require a high level of expertise, currently restricting metagenomic analysis to the realm of research, although that may soon change.…”

Section: Detection Methods For Antibiotic Resistance Monitoring Targetsmentioning

confidence: 99%

Antimicrobal Resistance: Fecal Sanitation Strategies for Combatting a Global Public Health Threat

Ashbolt¹,

Pruden²,

Miller³

et al. 2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Section: Detection Methods For Antibiotic Resistance Monitoring Targetsmentioning

confidence: 99%

Antimicrobal Resistance: Fecal Sanitation Strategies for Combatting a Global Public Health Threat

Ashbolt¹,

Pruden²,

Miller³

et al. 2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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“…Pyrosequencing combines standard PCR and sequencing by synthesis to rapidly determine the sequence of a target DNA region; it has been extensively used for the detection of bacterial resistance genes and bacterial community composition (Tang et al, 2016; Tian et al, 2016). This technique has been used to perform mutation analysis of bla SHV to resolve heterogeneous sequences in clinical isolates of K. pneumoniae containing more than one SHV variant (Haanperä et al, 2008).…”

Section: Detectionmentioning

confidence: 99%

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

2016

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β-lactamases are the primary cause of resistance to β-lactams among members of the family Enterobacteriaceae. SHV enzymes have emerged in Enterobacteriaceae causing infections in health care in the last decades of the Twentieth century, and they are now observed in isolates in different epidemiological settings both in human, animal and the environment. Likely originated from a chromosomal penicillinase of Klebsiella pneumoniae, SHV β-lactamases currently encompass a large number of allelic variants including extended-spectrum β-lactamases (ESBL), non-ESBL and several not classified variants. SHV enzymes have evolved from a narrow- to an extended-spectrum of hydrolyzing activity, including monobactams and carbapenems, as a result of amino acid changes that altered the configuration around the active site of the β -lactamases. SHV-ESBLs are usually encoded by self-transmissible plasmids that frequently carry resistance genes to other drug classes and have become widespread throughout the world in several Enterobacteriaceae, emphasizing their clinical significance.

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“…ARG was also greatly decreased by the biological wastewater treatment technique to the tune of 6.82% and 5.72% for activated sludge and final effluent respectively. The impact of WWTP on the water body was observed by increased ARG particularly at the downstream of the river [63]. Such environmental impact of antibiotic pollution viz-a-viz resistant genes hazard has been reported by some German researchers [64].…”

Section: Impact Of Arg Containing Wastewater On the Environmentmentioning

confidence: 72%

Threats from antibiotics: A serious environmental concern

Inyinbor

Bello

Fadiji

et al. 2018

Journal of Environmental Chemical Engineering

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Antibiotics are bioactive substances, used as human and animal medicines for illness prevention, disease treatment and growth promotion. They are considered to be pseudo persistent given their continuous input in the environment. Antibiotics enter agro ecosystems through several routes such as wastewater irrigation, soil application, animal manures or bio-solids which are mostly biologically active thus creating potential risks to the environment. They are present in different environmental matrices at low concentrations as residues. Antibiotic residues enter the environment primarily via urine, feces and manure from humans and animals after they have taken the medication, as well as from manufacturing wastewater. These residues contaminate the soil, surface water, and groundwater by leaching or runoff and ultimately breed antibiotic-resistant bacteria (ARB) and genes (ARG). These triune threat viz antibiotics, ARB and ARG have not been effectively removed by various treatment in wastewater treatment plants. Here, we put together existing knowledge and aim at providing in-depth knowledge to the extent to which a wide range of treatment processes determine the ultimate fate of antibioticbred threats (ARB and ARG) in conventional and advanced wastewater treatment. The use of antibiotics is inevitable, hence studies focusing on minimizing their discharge into the environment viz-a-viz support future regulatory measures are of great importance.

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Metagenomic analysis of bacterial community composition and antibiotic resistance genes in a wastewater treatment plant and its receiving surface water

Cited by 132 publications

References 64 publications

Antimicrobal Resistance: Fecal Sanitation Strategies for Combatting a Global Public Health Threat

Antimicrobal Resistance: Fecal Sanitation Strategies for Combatting a Global Public Health Threat

A Review of SHV Extended-Spectrum β-Lactamases: Neglected Yet Ubiquitous

Threats from antibiotics: A serious environmental concern

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