Metagenome Analysis as a Tool to Study Bacterial Infection Associated with Acute Surgical Abdomen

Abstract

Background: The purpose of this study was to profile the bacterium in the ascites and blood of patients with acute surgical abdomen by metagenome analysis. Methods: A total of 97 patients with acute surgical abdomen were included in this study. Accompanied with the standard culture procedures, ascites and blood samples were collected for metagenome analysis to measure the relative abundance of bacteria among groups of patients and between blood and ascites. Results: Metagenomic analysis identified 107 bacterial taxa from the ascites of patients. A principal component analysis (PCA) could separate the bacteria of ascites into roughly three groups: peptic ulcer, perforated or non-perforated appendicitis, and a group which included cholecystitis, small bowel lesion, and colon perforation. Significant correlation between the bacteria of blood and ascites was found in nine bacterial taxa both in blood and ascites with more than 500 sequence reads. However, the PCA failed to separate the variation in the bacteria of blood into different groups of patients, and the bacteria of metagenomic analysis is only partly in accordance with those isolated from a conventional culture method. Conclusion: This study indicated that the metagenome analysis can provide limited information regarding the bacteria in the ascites and blood of patients with acute surgical abdomen.

Integrated multi-omics reveal important roles of gut contents in intestinal ischemia–reperfusion induced injuries in rats

“…21d ). These results suggested a causal relation between E. coli and those significantly altered metabolites at IIR6h, which is further supported by several literatures, linking Enterobacteriaceae , and more specifically E. coli , to IIR associated diseases such as acute surgical abdomen 50 , small bowel transplantation 51 , necrotizing enterocolitis 52 – 54 , and sepsis 55 . Because IIR-induced remote organ injury is a complex physiological process associated with many factors, systematic and comprehensive explorations are still needed to further validate whether E.coli is truly the driver of downstream IIR-instigated complications.…”

Molecular characterization of endophytic fungal communities associated with Vitis vinifera L. at Taif region of Saudi Arabia

“…2c). Similar results of PCA analysis were obtained where PCA separated the bacteria of ascites from the bacteria of blood (Wu et al, 2018). Phylogenetic relationship among three locations was established based on the sequence difference in the ITS amplicon for endophytic fungi.…”

Whole metagenomic sequencing reveals different compositions of pathogenic bacteria in patients with appendicitis:Bacterial culture is not suitable as the only therapeutic guidance

“…Different acute abdomens harbor different bacterial species. Because of the similar bacterial species, appendicitis has similar pathogenesis among individuals (15). In the light of all the above de ciencies, it is di cult to make an optimal decision om antibiotic usage based on bacterial culture results alone.…”