Accurate detection of Helicobacter pylori infection and determination of antibiotics have significant meaning in clinical practice. The detection methods can be categorized into two types, invasive and non-invasive, but nowadays we use the urease breath test most frequently which is non-invasive. However, many developing countries cannot meet the requirements for having specialized equipment and they lack trained personnel. Also, for the children, it is difficult to make them cooperate for the test. Methods that detect Helicobacter pylori from stool sample can be a promising alternative for detection used in children and mass screening. Stool antigen tests have several advantages such as rapidity, simplicity, and cheapness, though their results may be influenced by the heterogenicity of antigens, the nature of biochemical techniques, and the amount of antigen presented in the stool. PCR-based methods can specifically detect Helicobacter pylori infection and antibiotic resistance by targeting specific gene sequence, but they also are limited by the requirements of facilities and experts, the existence of inhibitory substance, and interference from the dead bacteria. Some novel methods also deserve our attention. Here we summarized the results of researches about methods using stool sample and we hope our work can help clinicians choose the appropriate test in clinical practice.
Helicobacter pylori (H. pylori) is a helical, gram-negative, microaerophilic bacterium that is responsible for several gastroduodenal diseases, such as chronic gastritis, peptic ulcer, gastric mucosaassociated lymphoid tissue (MALT), and gastric adenocarcinoma. 1 It is estimated that more than 50% of the global population is infected with H. pylori, and it could be worse in developing countries given that the prevalence of H. pylori may be correlated with socioeconomic status. 2,3 It is widely known that H. pylori infection can be diagnosed by invasive and noninvasive methods. Noninvasive methods, such as the urea breath test and serological test, are widely applied in clinical practice. However, almost all the commonly used noninvasive
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