Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

Bjerrum, Jacob Tveiten; Nielsen, Ole Haagen; Hao, Fuhua; Tang, Huiru; Nicholson, Jeremy K.; Wang, Yulan; Olsen, Jørgen

doi:10.1021/pr9008223

Cited by 147 publications

(132 citation statements)

References 47 publications

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“…Besides fecal metabolite profiling, urine profiling can be performed as many microbial metabolites are absorbed from the colonic lumen and excreted in urine, either as such or after further processing by human enzymes. Several studies were able to discriminate IBD patients from HCs based on metabolite profiling of urine samples [40][41][42][43] , but not all [44] . Also, amino acids and amino acid-derived metabolites were found as discriminating compounds in urine.…”

Section: Ibd Disease Signature: Case-control Studiesmentioning

confidence: 99%

Metabolomics in the Clinical Diagnosis of Inflammatory Bowel Disease

Preter¹

2015

Dig Dis

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Background: Crohn's disease and ulcerative colitis represent the 2 major phenotypes of inflammatory bowel disease (IBD) that are characterized by chronic inflammation of all or parts of the gastrointestinal tract. The pathogenesis of both diseases is influenced by genetic predispositions as well as microbial and environmental factors. Currently, there is an emerging consensus hypothesis that a microbial dysbiosis is involved in initiating the disease or in maintaining it. These compositional alterations may be reflected in altered metabolic activities of the gut microbiota and has led to the use of ‘omic' profiling to improve the understanding of the pathophysiology of IBD. Key Messages: In the past few years, a metabolic approach has increasingly been applied in a number of studies of experimental and human IBD which were mostly focused on exploring disease-related metabolites to gain more insight into metabolic pathways. Metabolomics involves the high throughput identification, characterization and quantification of small molecule metabolites by different analytical techniques and has been performed in different biofluids such as serum/plasma, urine or fecal samples. The application of such a metabolite profiling technique has revealed different metabolites that allow the discrimination of IBD patients from healthy controls. In addition, separate IBD subtypes could be differentiated. Some of these metabolic changes were directly associated to alterations of specific gut microbial populations, implying a perturbation in the gut microbiome in the development of IBD. Conclusions: This review covers the emerging contribution of metabolomics for the discovery of an IBD signature and to identify biomarkers linked with a metabolic imbalance. For the implementation of metabolomics as a diagnostic tool in IBD, large prospective cohort studies are necessary.

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Section: Ibd Disease Signature: Case-control Studiesmentioning

confidence: 99%

Metabolomics in the Clinical Diagnosis of Inflammatory Bowel Disease

Preter¹

2015

Dig Dis

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“…Furthermore, stable isotope approaches make it possible to understand and measure flux through specific pathways, which provides the metabolic rate or metabolic path as opposed to the starting and final concentration of metabolites [14,15]. With this information, NMR-based metabolomics has been successfully used in toxicology [4,16,17], pathophysiology [18,19], understanding gene function [20], and for rapid diagnosis, therapeutics, and prognosis of metabolic disease using cell, animal models, and human samples [21,22] (Fig. 1a).…”

Section: This Article Is Part Of the Topical Collection On Pharmacomementioning

confidence: 99%

NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research

2016

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Purpose of Review In this review, the contribution of NMR to the metabolomics field will be discussed-particularly as it relates to cancer and drug metabolism research. An overview of the typical NMR metabolomic experiment will be presented while emphasizing important caveats ranging from experimental design to data interpretation. Recent Findings Despite some of the drawbacks of NMR, including reduced sensitivity compared to mass spectrometry-based approaches, NMR-based metabolomic approaches have provided considerable insight into drug and cancer metabolism. Advances in flux analysis based on the strategic use of isotopes have generated unique perspectives of cancer from the cellular and tissue levels. Summary Combined with other "omics" tools, including genomics, proteomics, and transcriptomics, metabolomic analysis completes the holistic view of metabolism. Many of these NMR-based approaches are likely to find their way into the clinic and become a common diagnostic tool.

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“…Permutation testing was also conducted to validate the predictability of the model [23]. If the maximum Q 2 obtained from the permutated models comparing controls and treatment-related groups did not exceed Q 2 obtained from the real model, the model suggested great predictability.…”

Section: Model Validation and Biomarker Selection In Opls-da Modelsmentioning

confidence: 99%

Rats’ Metabolic Responses to Chronic Intervention with Cantonese Herbal Tea

Li¹

2016

Metabolomics

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Cantonese Herbal Tea (CHT) is consumed in south China to alleviate feelings of discomfort due to the over-heat and humidity in the body, but the related molecular mechanisms behind its effects remain obscure. The urinary metabolic responses to CHT over time in rats were investigated during a 38-day-period using GC-MS-based metabonomics. Integrated with student's t-test and Orthogonal Projection on Latent Structures Discriminant Analysis (OPLS-DA) at each time point revealed that the process could be divided into two stages. In the first stage, the bioefficacy was characterized by elevated antioxidant activity and the depletion of oxidative stresses. However, the effect was reduced with time and replaced by a second stage characterized by the decreased flow of energy metabolism and an imbalance in gut microbiota. In conclusion, metabonomics study gained insight into the metabolic changes induced by CHT intake and the extent to which CHT intake influences the urinary metabolome, which also provides a powerful method for understanding the mechanism of CHT.

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Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the Pathophysiology

Cited by 147 publications

References 47 publications

Metabolomics in the Clinical Diagnosis of Inflammatory Bowel Disease

Metabolomics in the Clinical Diagnosis of Inflammatory Bowel Disease

NMR-Based Metabolomics and Its Application in Drug Metabolism and Cancer Research

Rats’ Metabolic Responses to Chronic Intervention with Cantonese Herbal Tea

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