Metabolomics unveils the influence of dietary phytochemicals on residual pesticide concentrations in honey bees

Ardalani, Hamidreza; Vidkjær, Nanna Hjort; Kryger, Per; Fiehn, Oliver; Fomsgaard, Inge S.

doi:10.1016/j.envint.2021.106503

Cited by 38 publications

(20 citation statements)

References 52 publications

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“…This faster metabolization may participate in the reduced sulfoxaflor toxicity upon ingestion of the S pollen diet. Such results also confirm a recent study, which reported that some pollens are better than others in promoting pesticide metabolization [49]. Different pollens have different nutritional values, which generally translate into differences in bee physiology and longevity [6,39,78,95,96].…”

Section: Discussionsupporting

confidence: 90%

“…Similarly, adding p-coumaric acid to a sucrose diet led to faster coumaphos disappearance [43]. Overall, these studies indicate that pollen may influence the ability of bees to metabolize pesticides, which was recently confirmed [49]. Lastly, we cannot exclude an influence of pollen on the ability of bees to withstand the effects of pesticides, given that the impact of pesticides depends not only on the fate of the molecule in the body (uptake, distribution, biotransformation, elimination), but also on its interaction with the biological target and effects at the physiological level.…”

Section: Introductionsupporting

confidence: 56%

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Pollen nutrition fosters honeybee tolerance to pesticides

et al. 2021

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A reduction in floral resource abundance and diversity is generally observed in agro-ecosystems, along with widespread exposure to pesticides. Therefore, a better understanding on how the availability and quality of pollen diets can modulate honeybee sensitivity to pesticides is required. For that purpose, we evaluated the toxicity of acute exposure and chronic exposures to field realistic and higher concentrations of azoxystrobin (fungicide) and sulfoxaflor (insecticide) in honeybees provided with pollen diets of differing qualities (named S and BQ pollens). We found that pollen intake reduced the toxicity of the acute doses of pesticides. Contrary to azoxystrobin, chronic exposures to sulfoxaflor increased by 1.5- to 12-fold bee mortality, which was reduced by pollen intake. Most importantly, the risk of death upon exposure to a high concentration of sulfoxaflor was significantly lower for the S pollen diet when compared with the BQ pollen diet. This reduced pesticide toxicity was associated with a higher gene expression of vitellogenin, a glycoprotein that promotes bee longevity, a faster sulfoxaflor metabolization and a lower concentration of the phytochemical p -coumaric acid, known to upregulate detoxification enzymes. Thus, our study revealed that pollen quality can influence the ability of bees to metabolize pesticides and withstand their detrimental effects, providing another strong argument for the restoration of suitable foraging habitat.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionsupporting

confidence: 56%

Pollen nutrition fosters honeybee tolerance to pesticides

et al. 2021

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“…For the MS condition, the samples were detected in targeted-selected ion monitoring (t-SIM) for quanti cation with the m/z 341.1555 ([M+H] + ) for COS 2 and m/z 502.2243 ([M+H] + ) for COS 3, which operated in a positive ionization mode and the parameters settings were as described in the literature (Ardalani et al 2021;Chitescu et al 2015). High-purity nitrogen was used as both the ion source and collision gas.…”

Section: Instrumentation and Analytical Conditionsmentioning

confidence: 99%

Pharmacokinetics, Bioavailability and Tissue Distribution of Chitobiose and Chitotriose in Rats

Chen

Jin

et al. 2021

Preprint

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Chitooligosaccharides (COSs) have various physiological activities and broad application prospects; however, their pharmacokinetics and tissue distribution remain unclear. In this study, a sensitive and selective ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for determining chitobiose (COS 2) and chitotriose (COS 3) in rat serum and tissues was developed. This method was successfully validated based on FDA guidelines in terms of selectivity, calibration curves (lower limit of quantification was 0.002 µg/mL for COS 2 and 0.02 µg/mL for COS 3), precision (intra-day relative standard deviation of 0.04–3.55% and inter-day relative standard deviation of 1.94–11.63%), accuracy (intra-day relative error of -1.81–11.06% and inter-day relative error of -9.41–8.63%), matrix effects, recovery (97.10–101.29%), stability, dilution integrity, and carry-over effects. Then, the method was successfully applied to the pharmacokinetics and tissue distribution study of COS 2 and COS 3 after intragastric and intravenous administration. After intragastric administration, COS 2 and COS 3 were rapidly absorbed, reached peak concentrations in the serum after approximately 0.45 h, and showed rapid elimination with clearances greater than 18.82 L/h/kg and half-lives lower than 6 h. The absolute oral bioavailability of COS 2 and COS 3 was 0.32–0.52%. COS 2 and COS 3 were widely distributed in Wistar rat tissues and could penetrated the blood-brain barrier without tissue accumulation.

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“…The study of aqueous metabolites of insects, which may involve whole insects or specific fluids, has been developing through the use of both mass spectrometry and NMR-based methods [4][5][6][7][8][9][10][11][12][13][14]. Studies may include a focus on specific organs such as the brain [4,5,10,15], which is particularly of interest because metabolic dysregulation has been attributed to this organ, particularly owing to neonicotinoids and pesticides [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…Metabolites 2021, 11, 864 2 of 14 Metabolomics has also been used to study insects in relationship to organisms in their surrounding environments, including interactions with phytochemicals [14,18], allowing for a wider perspective on honeybee colony collapses [7,19]. Despite the recent emergence of honeybee metabolomics, diverse applications are surfacing, such as honeybee gut microbiota studies [20,21], foodomics associated with honey [22] and royal jelly [23], and agricultural insecticide/pesticide effects [9,11,14,23]. Broadly, the discovery of biomarkers through studies of the metabolome of insects such as honeybees invites potential for improvements in health monitoring and stressor identification, addressing the honeybee colony crisis at hand [24].…”

Section: Introductionmentioning

confidence: 99%

Methodology for Single Bee and Bee Brain 1H-NMR Metabolomics

et al. 2021

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The feasibility of metabolomic 1H NMR spectroscopy is demonstrated for its potential to help unravel the complex factors that are impacting honeybee health and behavior. Targeted and non-targeted 1H NMR metabolic profiles of liquid and tissue samples of organisms could provide information on the pathology of infections and on environmentally induced stresses. This work reports on establishing extraction methods for NMR metabolic characterization of Apis mellifera, the European honeybee, describes the currently assignable aqueous metabolome, and gives examples of diverse samples (brain, head, body, whole bee) and biologically meaningful metabolic variation (drone, forager, day old, deformed wing virus). Both high-field (600 MHz) and low-field (80 MHz) methods are applicable, and 1H NMR can observe a useful subset of the metabolome of single bees using accessible NMR instrumentation (600 MHz, inverse room temperature probe) in order to avoid pooling several bees. Metabolite levels and changes can be measured by NMR in the bee brain, where dysregulation of metabolic processes has been implicated in colony collapse. For a targeted study, the ability to recover 10-hydroxy-2-decenoic acid in mandibular glands is shown, as well as markers of interest in the bee brain such as GABA (4-aminobutyrate), proline, and arginine. The findings here support the growing use of 1H NMR more broadly in bees, native pollinators, and insects.

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Metabolomics unveils the influence of dietary phytochemicals on residual pesticide concentrations in honey bees

Cited by 38 publications

References 52 publications

Pollen nutrition fosters honeybee tolerance to pesticides

Pollen nutrition fosters honeybee tolerance to pesticides

Pharmacokinetics, Bioavailability and Tissue Distribution of Chitobiose and Chitotriose in Rats

Methodology for Single Bee and Bee Brain 1H-NMR Metabolomics

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