Metabolomics investigation of molecular responses of whiteleg shrimp (Penaeus vannamei) to hypoxia

Abstract

Hypoxia is a common concern in shrimp aquaculture, affecting growth and survival. Although recent studies have revealed important insights into hypoxia in shrimp and crustaceans, knowledge gaps remain regarding this stressor at the molecular level. In the present study, a gas chromatography-mass spectrometry (GC-MS)-based metabolomics approach was employed to characterize the metabolic pathways underlying responses of shrimp (Penaeus vannamei) to hypoxia and to identify candidate biomarkers. We compared metabolite profiles of shrimp haemolymph before (0 h) and after exposure to hypoxia (1 & 2 h). Dissolved oxygen levels were maintained above 85% saturation in the control and before hypoxia, and 15% saturation in the hypoxic stress treatment. Results showed 44 metabolites in shrimp haemolymph that were significantly different between before and after hypoxia exposure. These metabolites were energy-related metabolites (e.g., TCA cycle intermediates, lactic acids, alanine), fatty acids and amino acids. Pathway analysis revealed 17 pathways that were significantly affected by hypoxia. The changes in metabolites and pathways indicate a shift from aerobic to anaerobic metabolism, disturbance in amino acid metabolism, osmoregulation, oxidative damage and Warburg effect-like response caused by hypoxic stress. Among the altered metabolites, lactic acid was most different between before and after hypoxia exposure. Biomarker analysis also indicated lactic acid as biomarker for hypoxia and model prediction. Future investigations may validate this molecule as a stress biomarker in aquaculture. This study contributes to better understanding of hypoxia in shrimp and crustaceans at the metabolic level and provides a base for future metabolomics investigations on hypoxia.

Effect of black soldier fly (Hermetia illucens) larvae meal on lipid and glucose metabolism of Pacific white shrimp Litopenaeus vannamei

“…Inhibition of the glycolytic pathway may affect the downstream TCA cycle and thus have an impact on energy production. The TCA cycle is one of the most important metabolic pathways in all aerobic organisms (68) . In the present study, a variety of intermediate metabolites associated with the TCA cycle were detected to show a decreasing trend, such as citrate, α-ketoglutarate, succinate and L-malic acid, indicating that dietary BSF disturbed the TCA cycle in shrimp.…”