Metabolomics as a complement to phylogenetics for assessing intraspecific boundaries in the desiccation-tolerant medicinal shrub Myrothamnus flabellifolia (Myrothamnaceae)

Bentley, Joanne; Moore, John P.; Farrant, Jill M.

doi:10.1016/j.phytochem.2018.12.016

Cited by 19 publications

(19 citation statements)

References 48 publications

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“…These results agree with our estimates for catechin content pattern. Finally, a rainfall gradient was associated to significant differences in polyphenols composition in an African medicinal shrub ( Myrothamnus flabellifolia ) for which metabolomic differences were congruent with the genetic structure of populations [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

Phenolic Compounds Content and Genetic Diversity at Population Level across the Natural Distribution Range of Bearberry (Arctostaphylos uva-ursi, Ericaceae) in the Iberian Peninsula

Asensio

Vitales

Pérez

et al. 2020

Plants

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Bearberry (Arctostaphylos uva-ursi) is a medicinal plant traditionally employed for the treatment of urinary tract infections due to high contents of arbutin (hydroquinone β-D-glucoside), which is now mainly used as a natural skin-whitening agent in cosmetics. Bearberry has also been proposed as a natural antioxidant additive due to the high contents of phenolic compounds in leaves. We studied the variation on phenolic compounds in 42 wild populations of bearberry, aiming to elucidate if intrinsic biological, climatic, and/or geographic factors affect phenolic contents across its natural distribution in the Iberian Peninsula. Bearberry leaves were collected during autumn over a three-year period (2014–2016) in populations across a latitude and altitude gradient. Methanolic extracts showed a wide range of variation in total phenols content, and different phenolic profiles regarding arbutin (levels of this major constituent varied from 87 to 232 mg/g dr wt), but also catechin and myricetin contents, which were affected by geographic and climatic factors. Moderate levels of variation on genome size—assessed by flow cytometry—and on two plastid DNA regions were also detected among populations. Genetic and cytogenetic differentiation of populations was weakly but significantly associated to phytochemical diversity. Elite bearberry genotypes with higher antioxidant capacity were subsequently identified.

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Section: Resultsmentioning

confidence: 99%

Phenolic Compounds Content and Genetic Diversity at Population Level across the Natural Distribution Range of Bearberry (Arctostaphylos uva-ursi, Ericaceae) in the Iberian Peninsula

Asensio

Vitales

Pérez

et al. 2020

Plants

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“…Compound 24 was attributed to quercetin-glucoside ( m / z 463) with a fragment at 301 (aglycone fragment). Quercetin-3- O -glucuronide, or miquelianin, was tentatively identified as 25 , with an m / z of 477 and characteristic fragment ions at 301 (aglycone fragment), 273, 257, 193, 179, and 151 and was previously found to have potential as a barcode for distinguishing M. flabellifolia material collected from Malawi and South Africa from material collected in Namibia [29]. Compound 26 ( m / z 469) could not be identified but was supposed to possibly represent a quercetin derivative due to the presence of a fragment ion at 301 representing the radical anion of the aglycone quercetin.…”

Section: Resultsmentioning

confidence: 99%

“…Galloyl quercetin-3- O -rhamnoside (compound 27 ) was putatively identified based on the characteristic fragment ions at 447, 301 (aglycone quercetin), and 171. Quercitrin [(quercetin 3- O -rhamnoside) compound 30 ] was identified based on the ion at m / z 447 and fragments at m / z 301 (aglycone fragment), 300 (radical aglycone anion), 271, 255, and 179 and constitutes a potential barcode for distinguishing Namibian M. flabellifolia material from the material from other regions [29]. Another compound with an m / z of 447 co-eluted with quercitrin and was deduced to represent methylellagic acid pentose based on the presence of the m / z 315 ion in the spectrum.…”

Section: Resultsmentioning

confidence: 99%

Metabolomic Profiling of the Desiccation-Tolerant Medicinal Shrub Myrothamnus flabellifolia Indicates Phenolic Variability Across Its Natural Habitat: Implications for Tea and Cosmetics Production

2019

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The leaves and twigs of the desiccation-tolerant medicinal shrub Myrothamnus flabellifolia are harvested for use in traditional and commercial teas and cosmetics due to their phenolic properties. The antioxidant and pharmacological value of this plant has been widely confirmed; however, previous studies typically based their findings on material collected from a single region. The existence of phenolic variability between plants from different geographical regions experiencing different rainfall regimes has thus not been sufficiently evaluated. Furthermore, the anthocyanins present in this plant have not been assessed. The present study thus used an untargeted liquid chromatography-tandem-mass spectrometry approach to profile phenolics in M. flabellifolia material collected from three climatically distinct (high, moderate, and low rainfall) regions representing the western, southern, and eastern extent of the species range in southern Africa. Forty-one putative phenolic compounds, primarily flavonoids, were detected, nine of which are anthocyanins. Several of these compounds are previously unknown from M. flabellifolia. Using multivariate statistics, samples from different regions could be distinguished by their phenolic profiles, supporting the existence of regional phenolic variability. This study indicates that significant phenolic variability exists across the range of M. flabellifolia, which should inform both commercial and traditional cultivation and harvesting strategies.

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“…3J1, the plant produces higher quantities of ROS scavengers, showing a special need to fight ROS, something that does not seem to be required in the presence of the 3J1 strain. The production of ROS scavengers is a common feature among desiccation-tolerant plants subjected to drying conditions ( Kranner and Birtic, 2005 ; França et al, 2007 ; Oliver et al, 2011 ; Challabathula et al, 2018 ; Bentley et al, 2019 ). Jiang and coworkers identified increased production of glutathione S-transferase in response to dehydration in B. hygrometrica , in special during the early stages of dehydration using proteome assays ( Jiang et al, 2007 ).…”

Section: Resultsmentioning

confidence: 99%

Proteome Comparison Between Natural Desiccation-Tolerant Plants and Drought-Protected Caspicum annuum Plants by Microbacterium sp. 3J1

2020

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Desiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for improving drought tolerance in crops. This understanding is especially relevant in an environment that tends to increase the number and intensity of droughts. The combination of certain microorganisms with drought-sensitive plants can improve their tolerance to water scarcity. One of these bacteria is Microbacterium sp. 3J1, an actinobacteria able to protect pepper plants from drought. In this study, we supplemented drought-tolerant and drought-sensitive plant rhizospheres with Microbacterium sp. 3J1 and analyzed their proteomes under drought to investigate the plant-microbe interaction. We also compare this root proteome with the proteome found in desiccation-tolerant plants. In addition, we studied the proteome of Microbacterium sp. 3J1 subjected to drought to analyze its contribution to the plantmicrobe interaction. We describe those mechanisms shared by desiccation-tolerant plants and sensitive plants protected by microorganisms focusing on protection against oxidative stress, and production of compatible solutes, plant hormones, and other more specific proteins.

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Metabolomics as a complement to phylogenetics for assessing intraspecific boundaries in the desiccation-tolerant medicinal shrub Myrothamnus flabellifolia (Myrothamnaceae)

Cited by 19 publications

References 48 publications

Phenolic Compounds Content and Genetic Diversity at Population Level across the Natural Distribution Range of Bearberry (Arctostaphylos uva-ursi, Ericaceae) in the Iberian Peninsula

Phenolic Compounds Content and Genetic Diversity at Population Level across the Natural Distribution Range of Bearberry (Arctostaphylos uva-ursi, Ericaceae) in the Iberian Peninsula

Metabolomic Profiling of the Desiccation-Tolerant Medicinal Shrub Myrothamnus flabellifolia Indicates Phenolic Variability Across Its Natural Habitat: Implications for Tea and Cosmetics Production

Proteome Comparison Between Natural Desiccation-Tolerant Plants and Drought-Protected Caspicum annuum Plants by Microbacterium sp. 3J1

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