Proteome Comparison Between Natural Desiccation-Tolerant Plants and Drought-Protected Caspicum annuum Plants by Microbacterium sp. 3J1

Abstract

Desiccation-tolerant plants are able to survive for extended periods of time in the absence of water. The molecular understanding of the mechanisms used by these plants to resist droughts can be of great value for improving drought tolerance in crops. This understanding is especially relevant in an environment that tends to increase the number and intensity of droughts. The combination of certain microorganisms with drought-sensitive plants can improve their tolerance to water scarcity. One of these bacteria is Microbacterium sp. 3J1, an actinobacteria able to protect pepper plants from drought. In this study, we supplemented drought-tolerant and drought-sensitive plant rhizospheres with Microbacterium sp. 3J1 and analyzed their proteomes under drought to investigate the plant-microbe interaction. We also compare this root proteome with the proteome found in desiccation-tolerant plants. In addition, we studied the proteome of Microbacterium sp. 3J1 subjected to drought to analyze its contribution to the plantmicrobe interaction. We describe those mechanisms shared by desiccation-tolerant plants and sensitive plants protected by microorganisms focusing on protection against oxidative stress, and production of compatible solutes, plant hormones, and other more specific proteins.

Microbial modulation of hormone signaling, proteomic dynamics, and metabolomics in plant drought adaptation

“…Aligned with this perspective, the investigation conducted by García‐Fontana et al. (2020) brought into focus the crucial contribution of Microbacterium sp. 3J1 in bolstering drought tolerance within pepper plants through the orchestration of hormone‐mediated proteomic transitions.…”

“…Finally, the investigation by García‐Fontana et al. (2020) pertinently explores the symbiotic interaction between plants and the drought‐protective bacterium Microbacterium sp. 3J1.…”

“…The plant responds by activating mechanisms that are also found in desiccation‐tolerant plants, such as protection against oxidative stress, osmotic adjustment, and cell wall remodeling. The plant‐microbe interaction results in improved plant growth and productivity under drought conditions (García‐Fontana et al., 2020). The red arrows: Up for positive regulation.…”

“…However, beneath the surface of the soil, within the intricate and often overlooked realm of plant roots, a remarkable drama unfolds. Here, the convergence of microbial activities plays a crucial role in orchestrating a delicate balance of hormonal signaling and proteomic dynamics in plant under drought condition (García‐Fontana et al., 2020). This microbiota orchestrates hormone‐mediated proteomic transitions, unveiling a series of proteome alterations that encompassed proteins related to oxidative stress defense, compatible solute synthesis, plant hormone modulation, and specific proteins involved in adapting to drought conditions.…”

Seed-Encapsulation of Desiccation-Tolerant Microorganisms for the Protection of Maize from Drought: Phenotyping Effects of a New Dry Bioformulation

“…Furthermore, xerotolerant strains are capable of producing and exuding exopolysaccharides, extracellular DNA (exDNA) and osmo-and xeroprotectant compounds that retain water in their structure and protect sensible structures. Thus, we have described strains of the Microbacterium, Arthrobacter, Pseudoarthrobacter, Leucobacter and Rhodococcus genera as capable of protecting pepper plants against drought using some of these mechanisms [15][16][17][18]. Moreover, some other strains of these genera were reported previously as drought stress tolerant [19][20][21].…”