Metabolomics approach to biomarkers of dry eye disease using <sup>1</sup>H-NMR in rats

Lee, Jung Dae; Kim, Hyang Yeon; Park, Jin Ju; Oh, Soo Bean; Goo, Hyeyoon; Cho, Kyong Jin; Kim, Suhkmann; Kim, Kyu‐Bong

doi:10.1080/15287394.2020.1867274

Cited by 11 publications

(4 citation statements)

References 77 publications

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“…Another study assessed rats with scopolamine-induced DED to characterize metabolic changes in eyes, urine, and serum for the exploration of biomarkers [ 51 ]. The authors found that 2-hydroxyisobutyrate, citrate, and succinate were significantly elevated in the plasma.…”

Section: Metabolomic Studies In Corneal Diseasesmentioning

confidence: 99%

Metabolomics in Corneal Diseases: A Narrative Review from Clinical Aspects

et al. 2023

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Corneal pathologies may have subtle manifestations in the initial stages, delaying diagnosis and timely treatment. This can lead to irreversible visual loss. Metabolomics is a rapidly developing field that allows the study of metabolites in a system, providing a complementary tool in the early diagnosis and management of corneal diseases. Early identification of biomarkers is key to prevent disease progression. The advancement of nuclear magnetic resonance and mass spectrometry allows the identification of new biomarkers in the analysis of tear, cornea, and aqueous humor. Novel perspectives on disease mechanisms are identified, which provide vital information for potential targeted therapies in the future. Current treatments are analyzed at a molecular level to offer further information regarding their efficacy. In this article, we provide a comprehensive review of the metabolomic studies undertaken in the cornea and various pathologies such as dry eye disease, Sjogren’s syndrome, keratoconus, post-refractive surgery, contact lens wearers, and diabetic corneas. Lastly, we discuss the exciting future that metabolomics plays in cornea research.

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Section: Metabolomic Studies In Corneal Diseasesmentioning

confidence: 99%

Metabolomics in Corneal Diseases: A Narrative Review from Clinical Aspects

et al. 2023

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“…Moreover, due to the multifactorial pathogenesis of dry eye, it is difficult to standardize the diagnosis of dry eye. The determination of the physiological characteristics and biomarkers of tears, including the pH (H 3 O + /OH − ) [ 13 ], electrolytes (Na + , Cl − ) [ 13 ], lysozyme [ 14 ], lactoferrin [ 14 , 15 ], interleukin proteins (IL-1α, IL-1β, and IL-17) [ 16 ], tumor necrotic factor (TNFα) [ 17 ], interferon gamma (IFNγ) [ 18 ], matrix metalloproteinase-9 (MMP-9) [ 19 ], and mucins [ 20 ], can provide a more critical determination of DED. These tests, however, are limited by the need for specialized equipment, poor environmental stability of protein assay kits, or the use of expensive molecular detection reagents (i.e., antibodies).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Hydrogels for the Diagnosis and Treatment of Dry Eye Disease

Cao

Wang

2022

Gels

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Dry eye disease (DED) is the most common clinical ocular surface disease. Given its multifactorial etiology, no consensus has been reached on the diagnosis criteria for dry eye disease. Topical drug administration remains the mainstay of treatment but is limited to the rapid clearance from the eye surface. To address these problems, hydrogel-based materials were designed to detect biomarkers or act as drug delivery systems by taking advantage of their good biocompatibility, excellent physical and mechanical properties, and long-term implant stability. Biosensors prepared using biocompatible hydrogels can be sensitive in diagnosing DED, and the designed hydrogels can also improve the drug bioavailability and retention time for more effective and long-term treatment. This review summarizes recent advances in the use of hydrogels for diagnosing and treating dry eye, aiming to provide a novel reference for the eventual clinical translation of hydrogels in the context of dry eye disease.

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“…In the present study, samples were analyzed using proton NMR ( 1 H-NMR), which can simultaneously measure several metabolites, and samples can be recovered and reused in other analytical instruments. Therefore, 1 H-NMR has been utilized in several research fields to identify biomarkers [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

A Metabolomics Approach to Sulforaphane Efficacy in Secondhand Smoking-Induced Pulmonary Damage in Mice

et al. 2022

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Sulforaphane is an isocyanate abundantly present in cruciferous vegetables. In the present study, we aimed to investigate the effects of sulforaphane on secondhand smoking (SHS)-induced pulmonary damage in mice. Additionally, a metabolomic study was performed to identify biomarkers associated with pulmonary disease using proton nuclear magnetic resonance (1H-NMR) analysis. Male C57BL6J mice were divided into a control group, an SHS exposure group (positive control group, PC), and a sulforaphane treatment group exposed to secondhand smoke (SS) (n = 5 per group). The PC and SS groups were exposed to secondhand smoke in a chamber twice daily for four weeks. Mice in the SS group were orally administered sulforaphane (50 mg/kg) for four weeks during secondhand smoke exposure. Histopathological examination of the lungs revealed pulmonary damage in PC mice, including loss of bronchial epithelial cells, bronchial wall thickening, and infiltration of macrophages. In contrast, mice in the SS group showed little or no epithelial thickening, thereby exhibiting reduced lung damage. Mouse serum and lung tissues were collected and analyzed to determine changes in endogenous metabolites using 1H-NMR. After target profiling, we identified metabolites showing the same tendency in the serum and lung as biomarkers for SHS-induced pulmonary damage, including taurine, glycerol, creatine, arginine, and leucine. As a result of histopathological examination, sulforaphane might inhibit SHS-induced lung damage, and metabolite analysis results suggest potential biomarkers for SHS-induced pulmonary damage in mice.

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Metabolomics approach to biomarkers of dry eye disease using ¹H-NMR in rats

Cited by 11 publications

References 77 publications

Metabolomics in Corneal Diseases: A Narrative Review from Clinical Aspects

Metabolomics in Corneal Diseases: A Narrative Review from Clinical Aspects

Recent Advances in Hydrogels for the Diagnosis and Treatment of Dry Eye Disease

A Metabolomics Approach to Sulforaphane Efficacy in Secondhand Smoking-Induced Pulmonary Damage in Mice

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Metabolomics approach to biomarkers of dry eye disease using 1H-NMR in rats

Cited by 11 publications

References 77 publications

Metabolomics in Corneal Diseases: A Narrative Review from Clinical Aspects

Metabolomics in Corneal Diseases: A Narrative Review from Clinical Aspects

Recent Advances in Hydrogels for the Diagnosis and Treatment of Dry Eye Disease

A Metabolomics Approach to Sulforaphane Efficacy in Secondhand Smoking-Induced Pulmonary Damage in Mice

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Product

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Metabolomics approach to biomarkers of dry eye disease using ¹H-NMR in rats