Metabolomics Approach Based on Multivariate Techniques for Blood Transfusion Reactions

Abstract

Blood transfusions temporarily improve the physical state of the patient but exert widespread effects on immune and non-immune systems. Perioperative allogeneic blood transfusions (ABT) are associated with various risks, including coagulopathy, incompatibility, transmission of infectious agents, and allergic reactions. Nevertheless, little is known about the global metabolic alterations that reflect the possible reactions of blood transfusions. In this study, we investigated metabolite changes generated by ABT in a rat model using metabolomics technology. To further profile the “metabolome” after blood transfusions, we used both liquid chromatography-quadrupole time-of-flight high-definition mass spectrometry and gas chromatography-mass spectrometry. ABT promoted a stimulatory microenvironment associated with a relative increase in glucose transporter 1/4 (GLUT1/GLUT4) expression. Supporting this result, glucose metabolism-related enzyme IRS1 and interleukin-6 (IL-6) were abnormally expressed, and levels of lysophosphatidylcholine (LysoPC) and its related enzyme phospholipase A2 (PLA2) were significantly altered in allogeneic groups compared to those in autologous groups. Finally, amino acid metabolism was also altered following ABT. Taken together, our results show a difference between autologous and allogeneic blood transfusions and demonstrate correlations with cancer-associated metabolic changes. Our data provide endogenous information for a better understanding of blood transfusion reactions.

Systemic and Local Metabolic Alterations in Sleep-Deprivation-Induced Stress: A Multiplatform Mass-Spectrometry-Based Lipidomics and Metabolomics Approach

“…We followed the established protocol for untargeted metabolomics using GC-MS with slight modifications adjusting for the concentrations of analyzed samples . The GC-MS analysis was conducted on a Shimadzu GCMS-QP2010 system (Shimadzu, Kyoto, Japan).…”

Caracterização epidemiológica das reações transfusionais imediatas em um hospital público de ensino

“…Apesar da eficiência do processo de transfusão e melhorias graduais na condição clínica dos pacientes transfundidos, o procedimento pode acarretar riscos de cunho imunológico e não imunológico (Lee et al, 2019). As reações transfusionais (RT) são provenientes das relações direta de hemocomponente (HC), considerando o processo de armazenamento e liberação de mediadores pró-inflamatórios, oriundo de um doador, pode causar problemas por incidentes durante o ato transfusional, reações decorrentes da constituição biofisiológica e genéticas de um paciente (Kim & Na, 2015).…”