Systemic and Local Metabolic Alterations in Sleep-Deprivation-Induced Stress: A Multiplatform Mass-Spectrometry-Based Lipidomics and Metabolomics Approach

Abstract

Sleep deprivation (SD) is known to be associated with metabolic disorders and chronic diseases. Complex metabolic alterations induced by SD at omics scale and the associated biomarker candidates have been proposed. However, in vivo systemic and local metabolic shift patterns of the metabolome and lipidome in acute and chronic partial SD models remain to be elucidated. In the present study, the serum, hypothalamus, and hippocampus CA1 of sleep-deprived rats (SD rats) from acute and chronic sleep restriction models were analyzed using three different omics platforms for the discovery and mechanistic assessment of systemic and local SD-induced dysregulated metabolites. We found a similar pattern of systemic metabolome alterations between two models, for which the area under the curve (AUC) of receiver operating characteristic curves was AUC = 0.847 and 0.930 with the pseudotargeted and untargeted metabolomics approach, respectively. However, SD-induced systemic lipidome alterations were significantly different and appeared to be model-dependent (AUC = 0.374). Comprehensive pathway analysis of the altered lipidome and metabolome in the hypothalamus indicated the abnormal behavior of eight metabolic and lipid metabolic pathways. The metabolic alterations of the hippocampus CA1 was subtle in two SD models. Collectively, these results extend our understanding of the quality of sleep and suggest metabolic targets in developing diagnostic biomarkers for better SD control.

Comprehensive Multi-Omics Analysis Reveals Aberrant Metabolism of Epstein–Barr-Virus-Associated Gastric Carcinoma

“…The information was acquired in positive ion mode. The MS condition was established following a previous study using auto MS/MS data-dependent acquisition mode and scan mode with proper modifications [28].…”

“Operator syndrome”: A unique constellation of medical and behavioral health-care needs of military special operation forces

“…Chronic sleep deprivation contributes to the increased risk of impaired immune functioning, Type 2 diabetes, heart disease, obesity, and chronic pain. 28 Conversely, research shows that good-quality sleep in people with TBI is protective. 29…”

Human blood serum proteome changes after 6 hours of sleep deprivation at night

“…Several groundbreaking transcriptomic studies as well as recent metabolomic studies have been published on animals (Weljie et al, 2015;Yoon et al, 2019;Ma et al, 2018;Poirrier et al, 2008;Seo et al, 2008) or humans (Archer et al, 2014;Davies et al, 2014;Moller-Levet et al, 2013;Pellegrino et al, 2012;Aho et al, 2016;Honma et al, 2020;Laing et al, 2017;Laing et al, 2019;Benedict et al, 2014). Moreover, the human circadian metabolome has been explored (Dallmann et al, 2012).…”