Metabolomics analysis of the protective effect of rubusoside on palmitic acid-induced lipotoxicity in INS-1 cells using UPLC-Q/TOF MS

Zheng, Heng; Wu, Jinxia; Huang, Hong; Meng, Chunmei; Li, Weidong; Wei, Tianli; Su, Zhiheng

doi:10.1039/c9mo00029a

Cited by 11 publications

(5 citation statements)

References 39 publications

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“…Similar to previously described, KEGG analysis was mainly enriched in metabolic pathways that are related to IR or diabetes, including metabolism of xenobiotics by cytochrome P450 ( 47 ), steroid hormone biosynthesis ( 48 ), retinol metabolism ( 49 ), ascorbate and aldarate metabolism ( 50 ), pentose and glucuronate interconversions ( 51 ), starch and sucrose metabolism. Several canonical pathways outstood among the statistics, including PI3K-Akt pathway, MAPK pathways and Rap1 signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

The transcriptomic and epigenetic alterations in type 2 diabetes mellitus patients of Chinese Tibetan and Han populations

et al. 2023

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BackgroundDue to the distinctive living environment, lifestyle, and diet, the Tibetan community in China has the lowest prevalence of T2DM and prediabetes among numerous ethnic groups, while Han community shows the highest statistic. In this study, we aim to conclude the clinical manifestations of both Tibetan and Han T2DM patients and their association with transcriptomic and epigenetic alterations.MethodsA cross-sectional study including 120 T2DM patients from Han and Tibetan ethnic groups were conducted between 2019 to 2021 at the Hospital of Chengdu University of Traditional Chinese Medicine. The various clinical features and laboratory tests were recorded and analyzed between the two groups. The genome-wide methylation pattern and RNA expression were determined by Reduced Representation Bisulfite Sequencing (RBBS) and Poly (A) RNA sequencing (RNA-seq) from leucocytes of peripheral blood samples in 6 Han and 6 Tibetan patients. GO analysis and KEGG analysis were conducted in differentially expressed genes and those with differentially methylated regions.ResultsCompared to Han, Tibetan T2DM individuals intake more coarse grains, meat and yak butter, but less refined grains, vegetables and fruit. They also showed increased BMI, Hb, HbA1c, LDL, ALT, GGT and eGFR, and decreased level of BUN. Among the 12 patients in the exploratory cohort, we identified 5178 hypomethylated and 4787 hypermethylated regions involving 1613 genes in the Tibetan group. RNA-seq showed a total of 947 differentially expressed genes (DEGs) between the two groups, with 523 up-regulated and 424 down-regulated in Tibetan patients. By integrating DNA methylation and RNA expression data, we identified 112 DEGs with differentially methylated regions (overlapping genes) and 14 DEGs with promoter-related DMRs. The functional enrichment analysis demonstrated that the overlapping genes were primarily involved in metabolic pathways, PI3K-Akt signaling pathway, MAPK signaling pathway, pathways in cancer and Rap1 signaling pathway.ConclusionOur study demonstrates the clinical characteristics of T2DM differ subtly between various ethnic groups that may be related to epigenetic modifications, thus providing evidence and ideas for additional research on the genetic pattern of T2DM.

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Section: Discussionmentioning

confidence: 99%

The transcriptomic and epigenetic alterations in type 2 diabetes mellitus patients of Chinese Tibetan and Han populations

et al. 2023

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“…Metabolomics is an important aspect of systems biology. Currently, commonly used analytic methods include partial least squares discriminant analysis (PLS‐DA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS‐DA) (Fond et al, 2013; Zheng et al, 2019). Through the analysis of serum, urine, feces and other biological samples, we could discuss the changes of small‐molecule metabolites in various metabolic pathways and the effect of natural active ingredients of drugs on disease treatment.…”

Section: Introductionmentioning

confidence: 99%

UPLC/Q‐TOF–MS‐based metabolomics evaluate the efficacy of oroxylin A against postmenopausal osteoporosis

Xian

Nong

Gao

et al. 2023

Biomedical Chromatography

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Post-menopausal osteoporosis (PMOP) is a common metabolic bone malady characterized by bone mass loss and bone microarchitectural deterioration; however, there is currently no effective drug for its management. According to our previous study, oroxylin A (OA) could effectively protect ovariectomized (OVX)osteoporotic mice from bone loss; however, its therapeutic targets are still unclear. From a metabolomic perspective, we studied serum metabolic profiles to discover potential biomarkers and OVX-related metabolic networks, which could assist us to comprehend the impact of OA on OVX. Five metabolites were identified as biomarkers associated with 10 related metabolic pathways, including phenylalanine, tyrosine and tryptophan biosynthesis, and phenylalanine, tryptophan and glycerophospholipid metabolism. After OA treatment, the expression of multiple biomarkers changed, with lysophosphatidylcholine (18:2) being a major significantly regulated biomarker. Our study demonstrated that OA's effects on OVX are probably related to the regulation of phenylalanine, tyrosine and tryptophan biosynthesis. Our findings explain the role of OA against PMOP in terms of metabolism and pharmacology and provide a pharmacological foundation for OA treatment of PMOP.

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“…Due to the low calories, safety and high sweetness of RS, it is used to replace sucrose and synthetic sweeteners. Recent research has demonstrated that RSs exhibit significant bioactivities, such as antiallergic (Wang et al, 2020), anti-carcinogenic (Chu et al, 2016) and anti-lipotoxic activities (Zheng et al, 2019). RSs can also achieve the solubilization of drugs (Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Directional bioconversion and optimization of stevioside into rubusoside by Lelliottia sp. LST-1

Huang

Lin

et al. 2022

Journal of Applied Microbiology

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Aims:The present study aimed to specifically transform stevioside (ST) into rubusoside (RS) through bioconversion with high efficiency, seeking to endow steviol glycosides (SGs) with subtle flavours for commercial acceptability. Methods and Results:An endophytic bacterium named Lelliottia LST-1 was screened and confirmed to specifically convert ST into RS, reaching a conversion rate of 75.4% after response surface optimization. Phylogenetic analysis combined with complete genome sequencing demonstrated that LST-1 was also presumed to be a new species. To further explore the principle and process of biological transformation, the potential beta-glucosidases GH3-1, GH3-2, GH3-3 and GH3-4 were expressed, purified and reacted with SGs. High-performance liquid chromatography revealed that all enzymes hydrolysed ST and generated RS, but substrate specificity analysis indicated that GH3-2 had the highest substrate specificity towards STs and the highest enzyme activity. Conclusion:The potential βglucosidase GH3-2 in Lelliottia sp. LST-1 was found to specifically and efficiently convert ST to RS. Significance and Impact of Study:The efficient biotransformation of ST into RS will be beneficial to its large-scale production and extensive application in the food and pharmaceutical industries.

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Metabolomics analysis of the protective effect of rubusoside on palmitic acid-induced lipotoxicity in INS-1 cells using UPLC-Q/TOF MS

Abstract: Diabetes is one of the most severe chronic diseases worldwide.

Cited by 11 publications

References 39 publications

The transcriptomic and epigenetic alterations in type 2 diabetes mellitus patients of Chinese Tibetan and Han populations

The transcriptomic and epigenetic alterations in type 2 diabetes mellitus patients of Chinese Tibetan and Han populations

UPLC/Q‐TOF–MS‐based metabolomics evaluate the efficacy of oroxylin A against postmenopausal osteoporosis

Directional bioconversion and optimization of stevioside into rubusoside by Lelliottia sp. LST-1

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