Metabolomics Analysis of the Lipid‐Regulating Effect of Allium hookeri in a Hamster Model of High‐Fat Diet‐Induced Hyperlipidemia by UPLC/ESI‐Q‐TOF Mass Spectrometry

Abstract

Hyperlipidemia is a risk factor for atherosclerotic cardiovascular disease and is a major public health concern. Allium hookeri (AH) is an Allium species containing high levels of bioactive organosulfur compounds such as methiin and cycloalliin. AH exerts hypolipidemic effects in animals fed a high-fat diet. However, there exists little information on the mechanisms underlying these effects. To address this issue, we used a metabolomic approach based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry to identify factors mediating the lipid-lowering effects of AH. Principal component and partial least-squares discriminant analyses of serum metabolome profiles revealed 25 metabolites as potential biomarkers for the effects of AH on lipid levels. These compounds were predominantly phospholipids, including phosphatidylcholines (PCs), lysoPCs, and lysophosphatidylethanolamines. Glycerophospholipid metabolism was identified as a significantly enriched pathway. These results provide mechanistic insight into the antihyperlipidemic effects of AH and evidence for its efficacy as a therapeutic agent.

The Effect of Simvastatin on Gut Microbiota and Lipid Metabolism in Hyperlipidemic Rats Induced by a High-Fat Diet

“…Bile acids are amphipathic molecules that are using cholesterol as a raw material and end products of cholesterol metabolism in the liver (Jang et al, 2018). Taurine is one of the important sulfonic acid amino acids in all mammalian tissues and has very important physiological functions such as bile acid conjugation, antioxidant and anti-inflammatory activity and ATP production (Zhou et al, 2015).…”

Effect of the polyphenol‐rich extract fromAllium cepaon hyperlipidemic sprague‐dawley rats

“…A clinical diagnosis of hyperlipidemia is made according to the observation of increased levels of TG, TC, and LDL‐C in combination with a reduction in HDL‐C level. Hyperlipidemia is mainly treated with hyperlipidemic agents including statins, in spite of the side effects and poor tolerance in some patients (Jang et al., 2018). Nevertheless, the antioxidant activity of some natural products such as polyphenols may effectively improve dyslipidemia, and thus offer an alternate treatment for hyperlipidemia (Li et al., 2019; NavaneethaKrishnan et al., 2019; Wang et al., 2018).…”

Allium hookeri Root Extract Inhibits Adipogenesis by Promoting Lipolysis in High Fat Diet-Induced Obese Mice

“…Our previous study has demonstrated that major components of AHR are alliin, S -allylcysteine, and phenolic compounds that are known to have anti-inflammatory [23,24,25] and antidiabetic effects [18,19,20]. A metabolomic study has shown that 25 metabolites altered by HFD-induced hyperlipidemia can be restored by AHR administration [33]. However, little is known about the impact of AHR on underlying cellular mechanism of adipogenesis, lipogenesis and lipolysis in HFD-induced obesity up to date.…”