Metabolomics analysis of sea cucumber (Apostichopus japonicus) in different geographical origins using UPLC–Q-TOF/MS

Zhao, Guanhua; Zhao, Wenda; Han, Lingshu; Ding, Jun; Chang, Yaqing

doi:10.1016/j.foodchem.2020.127453

Cited by 54 publications

(34 citation statements)

References 31 publications

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“…Moreover, variable importance in projection (VIP) is an important index. The differential constituents were selected based on VIP values > 1 and p -values from Student’s t -test < 0.05 [ 21 ]. Heatmap was applied to visualize the number of differential metabolites and to analyse their hierarchical cluster.…”

Section: Methodsmentioning

confidence: 99%

UHPLC–MS/MS Analysis on Flavonoids Composition in Astragalus membranaceus and Their Antioxidant Activity

et al. 2021

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Astragalus membranaceus is a valuable medicinal plant species widely distributed in Asia. Its root is the main medicinal tissue rich in methoxylated flavonoids. Origin can highly influence the chemical composition and bioactivity. To characterize the principal chemicals influenced by origin and provide more information about their antioxidant profile, the extracts of A. membranaceus roots from four origins were analysed by UHPLC-MS/MS. Thirty-four flavonoids, including thirteen methoxylated flavonoids, fifteen flavonoid glycosides and six flavonols, were identified. By principal component analysis, eighteen identified compounds were considered to be principal compounds. They could be used to differentiate A. membranaceus from Shanxi, Inner Mongolia, Heilongjiang and Gansu. The antioxidant activity was analysed by ORAC assay, DPPH radical scavenging activity assay and cell antioxidant activity assay. ‘Inner Mongolia’ extract showed the highest antioxidant activity. These results were helpful to understand how origin influenced the quality of A. membranaceus.

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Section: Methodsmentioning

confidence: 99%

UHPLC–MS/MS Analysis on Flavonoids Composition in Astragalus membranaceus and Their Antioxidant Activity

et al. 2021

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“…In a prior study of the geographical origin of tobacco, PCA conducted on metabolic analysis using gas chromatography/mass spectrometry (GC/MS) and capillary electrophoresis-mass spectrometry (CE/MS) showed evident discrimination [14]. The geographical origins of sea cucumbers from four different regions have also been identified by ultra-high performance liquid chromatography-quadrupole-time of flight/mass spectroscopy (UPLC-Q-TOF/MS) metabolomics [13]. Although there were differences between these studies and the present study, such as the species and analysis devices, they all appear to show that metabolomics can determine the geographical origins of living organisms.…”

Section: Differences In Organic Acid Metabolites In the Tricarboxylic...mentioning

confidence: 99%

“…For example, metabolomic analysis has been used to identify extra-virgin olive oils and sesame seeds from different geographical origins [11,12]. It has also been used to identify the origins of sea cucumbers using liquid chromatography/mass spectroscopy (LC/MS) [13]. Though many kinds of metabolomic analyses have been conducted to determine the geographical origins of seafood, to date, no such studies have focused on fish.…”

Section: Introductionmentioning

confidence: 99%

Analyzing the Metabolomic Profile of Yellowtail (Seriola quinquerdiata) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin

et al. 2021

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Country-of-origin violations have occurred in which some merchants have fraudulently sold cheap Japanese yellowtail (Seriola quinqueradiata) by presenting them as domestic Korean products. There are many methods for determining the origins of marine organisms, such as molecular genetic methods and isotope analysis. However, this study aimed to develop a method for determining the origins of aquatic products using metabolite analysis technology. Ten yellowtail each from Korea and Japan were analyzed by capillary electrophoresis–time of flight/mass spectrometry (CETOF/MS). Hierarchical cluster analysis (HCA) and principal component analysis (PCA) results showed highly differing aspects between the Korean and Japanese samples. In the tricarboxylic acid (TCA) cycle, citric, malic, oxaloglutaric, and fumaric acids exhibited significant differences between Korean and Japanese yellowtail. Sixteen of the twenty essential amino acids analyzed as metabolites also differed significantly. All amino acids were involved in protein digestion, absorption, and metabolism. All 16 amino acid contents were higher in Japanese yellowtail than in Korean yellowtail, except for glutamine. The fasting period was found to be the biggest factor contributing to the difference in amino acid contents, in addition to environmental factors (including feeding habits). These significant differences indicated that metabolomics could be used to determine geographical origin.

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“…Metabolomics allows for the collection of all metabolites in cells, tissues, or organisms under specific physiological periods or conditions [25]. Metabolomic signatures (fingerprints) can be obtained using different approaches, such as nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS) [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Metabolomics Approaches for the Comprehensive Evaluation of Fermented Foods: A Review

Gao

Hou

Gao

et al. 2021

Foods

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Fermentation is an important process that can provide new flavors and nutritional and functional foods, to deal with changing consumer preferences. Fermented foods have complex chemical components that can modulate unique qualitative properties. Consequently, monitoring the small molecular metabolites in fermented food is critical to clarify its qualitative properties and help deliver personalized nutrition. In recent years, the application of metabolomics to nutrition research of fermented foods has expanded. In this review, we examine the application of metabolomics technologies in food, with a primary focus on the different analytical approaches suitable for food metabolomics and discuss the advantages and disadvantages of these approaches. In addition, we summarize emerging studies applying metabolomics in the comprehensive analysis of the flavor, nutrition, function, and safety of fermented foods, as well as emphasize the applicability of metabolomics in characterizing the qualitative properties of fermented foods.

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Metabolomics analysis of sea cucumber (Apostichopus japonicus) in different geographical origins using UPLC–Q-TOF/MS

Cited by 54 publications

References 31 publications

UHPLC–MS/MS Analysis on Flavonoids Composition in Astragalus membranaceus and Their Antioxidant Activity

UHPLC–MS/MS Analysis on Flavonoids Composition in Astragalus membranaceus and Their Antioxidant Activity

Analyzing the Metabolomic Profile of Yellowtail (Seriola quinquerdiata) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin

Metabolomics Approaches for the Comprehensive Evaluation of Fermented Foods: A Review

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