UHPLC–MS/MS Analysis on Flavonoids Composition in Astragalus membranaceus and Their Antioxidant Activity

Abstract

Astragalus membranaceus is a valuable medicinal plant species widely distributed in Asia. Its root is the main medicinal tissue rich in methoxylated flavonoids. Origin can highly influence the chemical composition and bioactivity. To characterize the principal chemicals influenced by origin and provide more information about their antioxidant profile, the extracts of A. membranaceus roots from four origins were analysed by UHPLC-MS/MS. Thirty-four flavonoids, including thirteen methoxylated flavonoids, fifteen flavonoid glycosides and six flavonols, were identified. By principal component analysis, eighteen identified compounds were considered to be principal compounds. They could be used to differentiate A. membranaceus from Shanxi, Inner Mongolia, Heilongjiang and Gansu. The antioxidant activity was analysed by ORAC assay, DPPH radical scavenging activity assay and cell antioxidant activity assay. ‘Inner Mongolia’ extract showed the highest antioxidant activity. These results were helpful to understand how origin influenced the quality of A. membranaceus.

Astragalus membranaceus Additive Improves Serum Biochemical Parameters and Reproductive Performance in Postpartum Dairy Cows

“…The AM is a plant rich in various active substances, such as flavonoids, polysaccharides, and terpenoids ( 13 ). These active substances have various anti-inflammatory properties and antioxidant functions ( 33 ). After calving, the immunity of the dairy cows is at its lowest point, and concurrently, there are huge wounds within the reproductive tract ( 34 ).…”

Astragalus Mongholicus: A review of its anti-fibrosis properties

“…Besides, it shows an ability to improve intestinal fucntion ( Tian et al, 2021 ), peritoneal function ( Li et al, 2014b ), and anti-aging ability ( Gong et al, 2021 ). It has also been used for the prevention and treatment of osteoporosis ( Kaczmarczyk-Sedlak et al, 2013 ), antioxidant stress protection ( Sheng et al, 2021 ) and anti-radiation ( Wen et al, 2018 ). This review summarizes the relevant experimental studies on the anti-fibrosis effect of AM ( Figure 1 ).…”

Simultaneous determination and pharmacokinetic study of six components in beagle dog plasma by UPLC–MS/MS after oral administration of Astragalus Membranaceus aqueous extract

“…Astragalus Membranaceus contains isoflavones, flavonoid glycosides, saponins and so on, in which formononetin, calycosin‐7‐ O ‐glucoside (CG), ononin, astragaloside IV and astragaloside II are the main components (Chen et al, 2015; Liu et al, 2017; Sheng et al, 2021; Zhang et al, 2020). Recently, the low‐concern methylnissolin‐3‐ O ‐glucoside (MG) has been found to have antioxidant activity (Wu et al, 2021).…”