Metabolomics analysis of salt tolerance of <i>Zygosaccharomyces rouxii</i> and guided exogenous fatty acid addition for improved salt tolerance

Wang, Dingkang; Mi, Ting; Huang, Jun; Zhou, Rongqing; Yao, Jin; Wu, Chongde

doi:10.1002/jsfa.11975

Cited by 8 publications

(6 citation statements)

References 43 publications

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“…Metabolomics is closely related to phenotyping (Lv et al, 2023). There have been several studies on the effects of abiotic stress on plants, including drought (Zou et al, 2023), salt (Dingkang et al, 2022), extreme temperatures (Pei et al, 2021) and so on. Plants can accumulate specific secondary metabolites through certain mechanisms to resist the damage caused by stress, thereby improving their adaptability to the environment (Zamljen et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Zhang,

et al. 2024

Physiologia Plantarum

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Plant growth is restricted by salt stress, which is a significant abiotic factor, particularly during the seedling stage. The aim of this study was to investigate the mechanisms underlying peanut adaptation to salt stress by transcriptomic and metabolomic analysis during the seedling stage. In this study, phenotypic variations of FH23 and NH5, two peanut varieties with contrasting tolerance to salt, changed obviously, with the strongest differences observed at 24 h. FH23 leaves wilted and the membrane system was seriously damaged. A total of 1470 metabolites were identified, with flavonoids being the most common (21.22%). Multi‐omics analyses demonstrated that flavonoid biosynthesis (ko00941), isoflavones biosynthesis (ko00943), and plant hormone signal transduction (ko04075) were key metabolic pathways. The comparison of metabolites in isoflavone biosynthesis pathways of peanut varieties with different salt tolerant levels demonstrated that the accumulation of naringenin and formononetin may be the key metabolite leading to their different tolerance. Using our transcriptomic data, we identified three possible reasons for the difference in salt tolerance between the two varieties: (1) differential expression of LOC112715558 (HIDH) and LOC112709716 (HCT), (2) differential expression of LOC112719763 (PYR/PYL) and LOC112764051 (ABF) in the abscisic acid (ABA) signal transduction pathway, then (3) differential expression of genes encoding JAZ proteins (LOC112696383 and LOC112790545). Key metabolites and candidate genes related to improving the salt tolerance in peanuts were screened to promote the study of the responses of peanuts to NaCl stress and guide their genetic improvement.

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Section: Discussionmentioning

confidence: 99%

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Zhang,

et al. 2024

Physiologia Plantarum

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“…In particular, Z. rouxii can grow and survive under pressure caused by high concentrations of non–ionic (sugars and polyols) and ionic (mainly Na + cations) solutes [ 7 , 8 ]. When Z. rouxii cells are stressed by high glucose and high salt, they activate stress signaling pathways, such as the HOG pathway, the calcineurin/Crz1 pathway, and the Ras–cAMP pathway [ 5 , 7 , 9 ]; produce and accumulate metabolically compatible osmotic and protective agents, such as trehalose, xylitol, and glycerol [ 10 , 11 ]; and regulate the structural characteristics of the cell wall and plasma membrane, as well as adjusting the transport system [ 12 , 13 ]. All of these improve the tolerance of Z. rouxii cells and determine the success of fermentation on high–osmotic food substrates [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

High Glucose Is a Stimulation Signal of the Salt–Tolerant Yeast Zygosaccharomyces rouxii on Thermoadaptive Growth

Yan,

Xiao,

Liu

et al. 2024

JoF

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The salt–tolerant yeast Zygosaccharomyces rouxii is a typical aroma–producing yeast used in food brewing, but its mechanism of high temperature tolerance is still unclear. In this study, the response mechanism of Z. rouxii to glucose under high temperature stress at 40 °C was explored, based on the total synthetic lowest–nutrient medium. The results of the growth curves and scanning electron microscopy showed that high glucose was necessary for Z. rouxii to restore growth under high temperature stress, with the biomass at 300 g/L of glucose (OD600, 120h = 2.44 ± 0.26) being 8.71 times higher than that at 20 g/L (OD600, 120h = 0.28 ± 0.08). The results of the transcriptome analysis, combined with RT–qPCR, showed that the KEGG analysis of differentially expressed genes was enriched in pathways related to glucose metabolism, and high glucose (300 g/L) could effectively stimulate the gene expression of glucose transporters, trehalose synthesis pathways, and xylitol synthesis pathways under a high temperature, especially the expression of the glucose receptor gene RGT2 (up–regulated 193.7 times at 12 h). The corresponding metabolic characteristics showed that the contents of intracellular metabolites, such as glucose (Cmax, 6h = 6.50 ± 0.12 mg/g DCW), trehalose (Cmax, 8h = 369.00 ± 17.82 μg/g DCW), xylitol (Cmax, 8h = 1.79 ± 0.27 mg/g DCW), and glycerol (Cmax, 8h = 268.10 ± 44.49 μg/g DCW), also increased with time. The accumulation of acetic acid, as the main product of overflow metabolism under high temperature stress (intracellular Cmax, 2h = 126.30 ± 10.96 μg/g DCW; extracellular Cmax, 12h = 499.63 ± 27.16 mg/L), indicated that the downstream glycolysis pathway was active. Compared with the normal physiological concentration of glucose, a high glucose concentration can effectively stimulate the gene expression and metabolism of salt–tolerant Z. rouxii under high–temperature conditions to restore growth. This study helps to deepen the current understanding of the thermoadaptive growth mechanism of salt–tolerant Z. rouxii.

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“…Efficiencies of cell proliferation and product accumulation may be impacted by the stressful environments encountered by cells during fermentation 5,6 . Previous research demonstrated that salt stress enhanced unsaturated fatty acid production in Zygosaccharomyces rouxii , and cells were more tolerant to salt when supplemented with unsaturated fatty acids 7,8 . Consequently, engineering the synthesis of unsaturated fatty acids is a potential strategy for enhancing the stress resistance of cells.…”

Section: Introductionmentioning

confidence: 99%

Engineering the synthesis of unsaturated fatty acids by introducing desaturase improved the stress tolerance of yeast

Wang,

Hao,

Jiao

et al. 2023

J Sci Food Agric

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BACKGROUNDYeast is often used to build cell factories to produce various chemicals or nutrient substances, which means the yeast has to encounter stressful environments. Previous research reported that unsaturated fatty acids were closely related to yeast stress resistance. Engineering unsaturated fatty acids may be a viable strategy for enhancing the stress resistance of cells.RESULTSIn this study, two desaturase genes, OLE1 and FAD2 from Z. rouxii, were overexpressed in S. cerevisiae to determine how unsaturated fatty acids affect cellular stress tolerance of cells. After cloning and plasmid recombination, the recombinant S. cerevisiae cells were constructed. Analysis of membrane fatty acid contents revealed that the recombinant S. cerevisiae with overexpression of OLE1 and FAD2 genes contained higher levels of fatty acids C16:1 (2.77 times), C18:1 (1.51 times) and C18:2 (4.15 times) than the wild‐type S. cerevisiae pY15TEF1. In addition, recombinant S. cerevisiae cells were more resistant to multiple stresses, and exhibited improved membrane functionality, including membrane fluidity and integrity.CONCLUSIONThese findings demonstrated that strengthening the expression of desaturases was beneficial to stress tolerance. Overall, this study may provide a suitable means to build a cell factory of industrial yeast cells with high tolerance during biological manufacturing. © 2023 Society of Chemical Industry.

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Metabolomics analysis of salt tolerance of Zygosaccharomyces rouxii and guided exogenous fatty acid addition for improved salt tolerance

Cited by 8 publications

References 43 publications

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

Integrative multi‐omics analysis reveals the crucial biological pathways involved in the adaptive response to NaCl stress in peanut seedlings

High Glucose Is a Stimulation Signal of the Salt–Tolerant Yeast Zygosaccharomyces rouxii on Thermoadaptive Growth

Engineering the synthesis of unsaturated fatty acids by introducing desaturase improved the stress tolerance of yeast

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