Metabolomics analysis of nitrogen-containing metabolites between two Dendrobium plants

Song, Cheng; Chen, Jiao; Jin, Qing; Chen, Cunwu; Cai, Yongping; Lin, Yi

doi:10.1007/s12298-020-00822-1

Cited by 18 publications

(17 citation statements)

References 27 publications

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“…For example, the comparative metabolite analysis of Dendrobium officinale Kimura et Migo and Dendrobium huoshanense Z. Z. Tang et S. J. Cheng showed that the accumulation of alkaloids was species-specific ( Song et al, 2020 ). Ten potential anti-inflammatory alkaloid components were detected from the extraction of Dendrobium aphyllum (Roxb.)…”

Section: Structural Identification Of Dendrobium Alkaloidsmentioning

confidence: 99%

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Mou

Zhao

et al. 2021

Front. Pharmacol.

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Dendrobium is a genus of flowering plants belonging to the Orchidaceae family with more than 1,400 species. Many Dendrobium species have been used as medicinal plants in several Asian countries for thousands of years. Alkaloids were reported as the major biological markers due to their complex chemical compositions and various types. In this review, we summarized the structural types of alkaloids, their pharmacological activities, as well as the mechanisms of biological activities. More than sixty alkaloids were isolated and identified from the Dendrobium genus. Moreover, the pharmacological effects of Dendrobium alkaloids as hepatic lipid and gluconeogenesis regulation, as neuroprotection, and as anti-tumor, anti-inflammatory, anti-diabetes, and anti-virus factors were described. Besides, the total chemical synthesis of dendrobine is provided, while the biosynthetic pathway of dendrobine has been proposed based on the functions of associated genes. For applications of these invaluable herbs, more researches on the extraction of biological markers from compounds are needed. Further confirmation of the proposed biosynthetic pathways is anticipated as well.

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Section: Structural Identification Of Dendrobium Alkaloidsmentioning

confidence: 99%

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Mou

Zhao

et al. 2021

Front. Pharmacol.

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“…To optimize the condition of mass spectrometry, the suitable CID energy was achieved by the direct infusion experiment. Generally, the strongest fragment ions and the smallest number of fragments were observed at 30-35% collision energy, whereas the abundances of the fragments decreased substantially at 45% or greater collision energy (Song et al 2020). In this experiment, sarpagine and reserpine achieved sufficient fragments both in quantity and intensity at 40% collision energy.…”

Section: Resultsmentioning

confidence: 99%

“…Thus far, the composition and biosynthetic pathway of the alkaloids in D. officinale has not been entirely understood. The determination of the alkaloids between D. officinale and D. nobile using an LC-MS method showed that 25 identical compositions in the two Dendrobium plants were tentatively identified; however, dendrobine was only detected in D. nobile (Song et al, 2020). Using a UPLC method, a few coumarins, alkaloids and bibenzyl components from fifteen Dendrobium species were quantitatively determined.…”

Section: Introductionmentioning

confidence: 99%

Identification of Alkaloids and Related Intermediates of Dendrobium officinale by Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography Tandem Mass Spectrometry

Song

Zhang

Manzoor

et al. 2022

Preprint

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JA signaling plays a pivotal role in plant stress responses and secondary metabolism. Many studies have demonstrated that jasmonates effectively induce the expressions of alkaloid biosynthesis genes in various plants, which rendered to the accumulation of alkaloid to counteract stresses. Despite the multiple roles of JAs in the regulation of plant growth and different stresses, less studied involved in the regulatory role of JA in Dendrobium officinale. Here, a strategy for the rapid identification of alkaloid and precursors of D. officinale was established based on a solid-phase extraction-high performance liquid chromatography-linear ion trap- Orbitrap tandem mass spectrometry (SPE-HPLC-LTQ-Orbitrap) method. The potential compounds were tentatively identified by aligning the accurate molecular weight with the METLIN and Dictionary of Natural Products databases. The chemical structures and main characteristic fragments of the potential compounds were further confirmed by retrieving the multistage mass spectra from the MassBank and METLIN databases.The Mass Frontier software was used to speculate the fragmentation pathway of the identified compounds. As a result, seven alkaloids were separated and identified from D. officinale, which were mainly classified into five types (tropane alkaloids, tetrahydroisoquinoline alkaloids, quinolizidine alkaloids, piperidine alkaloids and spermidine alkaloids). Besides the alkaloids, forty-nine chemical substances, including guanidines, nucleotides, dipeptides, sphingolipids and nitrogen-containing glucosides, were concurrently identified in the experiment. Taken together, this study revealed the composition of chemicals currently found in D. officinale, which could provide the scientific method for the identification of alkaloids in other Dendrobium plants.

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“…There were 11 secondary metabolites that were considered as biomarkers of D. officinale and Dendrobium huoshanense ( Jin et al, 2016 ). Moreover, in these two species, as many as 133 nitrogenous compounds were identified, and allyl alkaloid is an important medicinal component worth further investigation ( Song et al, 2020 ). For D. officinale plants, the third year is the most suitable harvest time based on the accumulation of metabolites ( Jin et al, 2016 ).…”

Section: Metabolomic Research In Dendrobium Officinalementioning

confidence: 99%

Research Advances in Multi-Omics on the Traditional Chinese Herb Dendrobium officinale

et al. 2022

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Dendrobium officinale Kimura et Migo is an important epiphytic plant, belonging to the Orchidaceae family. There are various bioactive components in D. officinale plants, mainly including polysaccharides, alkaloids, and phenolic compounds. These compounds have been demonstrated to possess multiple functions, such as anti-oxidation, immune regulation, and anti-cancer. Due to serious shortages of wild resources, deterioration of cultivated germplasm and the unstable quality of D. officinale, the study has been focused on the biosynthetic pathway and regulation mechanisms of bioactive compounds. In recent years, with rapid developments in detection technologies and analysis tools, omics research including genomics, transcriptomics, proteomics and metabolomics have all been widely applied in various medicinal plants, including D. officinale. Many important advances have been achieved in D. officinale research, such as chromosome-level reference genome assembly and the identification of key genes involved in the biosynthesis of active components. In this review, we summarize the latest research advances in D. officinale based on multiple omics studies. At the same time, we discuss limitations of the current research. Finally, we put forward prospective topics in need of further study on D. officinale.

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Metabolomics analysis of nitrogen-containing metabolites between two Dendrobium plants

Cited by 18 publications

References 27 publications

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Identification, Biological Activities and Biosynthetic Pathway of Dendrobium Alkaloids

Identification of Alkaloids and Related Intermediates of Dendrobium officinale by Solid-Phase Extraction Coupled with High-Performance Liquid Chromatography Tandem Mass Spectrometry

Research Advances in Multi-Omics on the Traditional Chinese Herb Dendrobium officinale

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