Metabolomics Analysis of Laparoscopic Surgery Combined with Wuda Granule to Promote Rapid Recovery of Patients with Colorectal Cancer Using UPLC/Q‐TOF‐MS/MS

Abstract

Surgery is the primary curative treatment for patients with nonmetastasized colorectal cancer (CRC). Rate of complications, morbidity, mortality, and overall survival of patients with CRC are factors associated with speed of recovery following surgery. Wuda granule (WD) is a traditional Chinese medicine (TCM) prescription used to promote rapid recovery after surgery. However, the specific mechanism of action of WD has not been characterized. Our study included 60 patients with clear histopathological evidence of colon or rectal cancer who underwent CRC laparoscopic surgery and 30 healthy individuals. Serum biochemistry and clinical evaluation of gastrointestinal function showed that WD could improve the nutritional status and gastrointestinal function and reduce the level of inflammation of patients with CRC following laparoscopic surgery. In addition, we used UPLC/Q-TOF-MS/MS-based metabolomics analysis to determine the mechanism of WD-related rapid recovery following laparoscopic surgery in patients with CRC. Twenty metabolites associated with arachidonic acid, alanine, aspartate and glutamate, α-linolenic acid, pyruvate, histidine, and glycerophospholipids were identified. The results suggested that the therapeutic mechanism of laparoscopic surgery combined with WD may be related to regulation of nutritional status, inflammation, immune function, energy, and gastrointestinal function in patients with CRC. This study also highlighted the ability of TCM compounds to interact with multiple targets to induce synergistic effects. This study may result in further studies of WD as a therapeutic agent to promote recovery following surgical resection of CRC tumors.

Efficacy of Zhuyu Pill Intervention in a Cholestasis Rat Model: Mutual Effects on Fecal Metabolism and Microbial Diversity

“…Metabonomics has been used to study the organism as a whole, which is consistent with the holistic view of TCM and the concept of syndrome differentiation and treatment ( Wang et al, 2017 ). Because the modernization of TCM is becoming necessary and urgent ( Li et al, 2008 ), metabolomics represents a modern technology with a great potential toward understanding the efficacy and mechanism of TCM ( Sun et al, 2012 ; Wang et al, 2020 ).…”

Pathway‐based protein–protein association network to explore mechanism ofα‐glucosidase inhibitors fromScutellaria baicalensisGeorgi against type 2 diabetes

“…[ 58 ]. In the authors’ previous works [ 14 ], they conducted a network analysis of the targets of AGIs from SBG and that of commercial drugs for T2D. The interaction data were also calculated by the STRING database.…”

“…In the authors’ previous studies [ 14 ], 13 α‐glucosidase inhibitors (AGIs) were identified from SBG by ultrafiltration LC‐MS method, including tenaxin I, skullcapflavone II, viscidulin III, etc. Detailed information about these compounds is listed in Table S1 .…”

“…Extract of this plant has been reported to show an α‐glucosidase inhibition activity [ 5 ]. In the authors’ previous works [ 14 ], a total of 32 flavonoids from the root of SBG were extracted and identified. Moreover, 13 of them were exhibited as α‐glucosidase inhibitors, including wogonin, chrysin, and oroxylin A, etc.…”

Early Warning of Ischemic Stroke Based on Atherosclerosis Index Combined With Serum Markers

“…Metabolisms (or metabonomics) is a discipline that developed after proteomics and genomics ( 15 ). It observes the dynamic changes of metabolites before and after the biological system is stimulated or disturbed, and uses various analytical tools to obtain the metabolic network of the biological system ( 16 ).…”