Metabolomics analysis of exhaled breath condensate for discrimination between lung cancer patients and risk factor individuals

Peralbo‐Molina, Ángela; Calderón-Santiago, M.; Priego‐Capote, Feliciano; Jurado-Gámez, Bernabé; Castro, M. D. Luque de

doi:10.1088/1752-7155/10/1/016011

Cited by 27 publications

(18 citation statements)

References 44 publications

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“…Squalene is also present in exhaled breath condensate and, at this level, can be used to discriminate between a cohort of patients with lung cancer, risk factor subjects (active smokers and ex‐smokers), and healthy individuals …”

Section: Squalene In Other Locationsmentioning

confidence: 99%

Current Insights into the Biological Action of Squalene

Lou-Bonafonte

Martínez‐Beamonte

Sanclemente

et al. 2018

Molecular Nutrition Food Res

115

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Squalene is a triterpenic compound found in a large number of plants and other sources with a long tradition of research since it was first reported in 1926. Herein a systematic review of studies concerning squalene published in the last 8 years is presented. These studies have provided further support for its antioxidant, anti-inflammatory, and anti-atherosclerotic properties in vivo and in vitro. Moreover, an antineoplastic effect in nutrigenetic-type treatments, which depends on the failing metabolic pathway of tumors, has also been reported. The bioavailability of squalene in cell cultures, animal models, and in humans has been well established, and further progress has been made in regard to the intracellular transport of this lipophilic molecule. Squalene accumulates in the liver and decreases hepatic cholesterol and triglycerides, with these actions being exerted via a complex network of changes in gene expression at both transcriptional and post-transcriptional levels. Its presence in different biological fluids has also been studied. The combination of squalene with other bioactive compounds has been shown to enhance its pleiotropic properties and might lead to the formulation of functional foods and nutraceuticals to control oxidative stress and, therefore, numerous age-related diseases in human and veterinary medicine.

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Section: Squalene In Other Locationsmentioning

confidence: 99%

Current Insights into the Biological Action of Squalene

Lou-Bonafonte

Martínez‐Beamonte

Sanclemente

et al. 2018

Molecular Nutrition Food Res

115

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“…Just in the case of cancer has metabolomics been applied to study lung, kidney, prostate, gastric, colorectal, ovarian [5], liver, brain [6], and breast cancer tissues [7]. Among other types of mammalian samples metabolomics studies on cerebrospinal [8] or amniotic [9] fluids, exhaled breath condensate [10], stool, or saliva [11] samples were performed.…”

Section: Introductionmentioning

confidence: 99%

LC-MS-Based Metabolic Fingerprinting of Aqueous Humor

Pietrowska

Dmuchowska

Samczuk

et al. 2017

Journal of Analytical Methods in Chemistry

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Aqueous humor (AH) is a transparent fluid which fills the anterior and posterior chambers of the eye. It supplies nutrients and removes metabolic waste from avascular tissues in the eye. Proper homeostasis of AH is required to maintain adequate intraocular pressure as well as optical and refractive properties of the eye. Application of metabolomics to study human AH may improve knowledge about the molecular mechanisms of eye diseases. Until now, global analysis of metabolites in AH has been mainly performed using NMR. Among the analytical platforms used in metabolomics, LC-MS allows for the highest metabolome coverage. The aim of this study was to develop a method for extraction and analysis of AH metabolites by LC-QTOF-MS. Different protocols for AH preparation were tested. The best results were obtained when one volume of AH was mixed with one volume of methanol : ethanol (1 : 1). In the final method, 2 µL of extracted sample was analyzed by LC-QTOF-MS. The method allowed for reproducible measurement of over 1000 metabolic features. Almost 250 metabolites were identified in AH and assigned to 47 metabolic pathways. This method is suitable to study the potential role of amino acids, lipids, oxidative stress, or microbial metabolites in development of ocular diseases.

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“…However, in almost all cases, the prediction in terms of analysis sensitivity and specificity of these tests have been limited [ 125 ]. Recent research conducted by Peralbo-Molina et al [ 126 ] involved the analysis of breath samples from smoking, non-smoking and past smoking patients in order to detect the metabolic signature within exhaled breath condensate for risk and cancer affected individuals. In addition to the previously detected alkanes [ 125 ], a high resolution GC-TOF-MS approach was able to identify monoglycerols and squalene as the prominent signature metabolites.…”

Section: Breathomics and Salivaomics Applicationsmentioning

confidence: 99%

A Review of Analytical Techniques and Their Application in Disease Diagnosis in Breathomics and Salivaomics Research

Beale

Jones

Karpe

et al. 2016

IJMS

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The application of metabolomics to biological samples has been a key focus in systems biology research, which is aimed at the development of rapid diagnostic methods and the creation of personalized medicine. More recently, there has been a strong focus towards this approach applied to non-invasively acquired samples, such as saliva and exhaled breath. The analysis of these biological samples, in conjunction with other sample types and traditional diagnostic tests, has resulted in faster and more reliable characterization of a range of health disorders and diseases. As the sampling process involved in collecting exhaled breath and saliva is non-intrusive as well as comparatively low-cost and uses a series of widely accepted methods, it provides researchers with easy access to the metabolites secreted by the human body. Owing to its accuracy and rapid nature, metabolomic analysis of saliva and breath (known as salivaomics and breathomics, respectively) is a rapidly growing field and has shown potential to be effective in detecting and diagnosing the early stages of numerous diseases and infections in preclinical studies. This review discusses the various collection and analyses methods currently applied in two of the least used non-invasive sample types in metabolomics, specifically their application in salivaomics and breathomics research. Some of the salient research completed in this field to date is also assessed and discussed in order to provide a basis to advocate their use and possible future scientific directions.

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Metabolomics analysis of exhaled breath condensate for discrimination between lung cancer patients and risk factor individuals

Cited by 27 publications

References 44 publications

Current Insights into the Biological Action of Squalene

Current Insights into the Biological Action of Squalene

LC-MS-Based Metabolic Fingerprinting of Aqueous Humor

A Review of Analytical Techniques and Their Application in Disease Diagnosis in Breathomics and Salivaomics Research

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