Metabolomic profiling of lung and prostate tumor tissues by capillary electrophoresis time-of-flight mass spectrometry

Kami, Kenjiro; Fujimori, Tamaki; Sato, Hajime; Sato, Munetake; Yamamoto, Hiroyuki; Ohashi, Yoshiaki; Sugiyama, Naoyuki; Ishihama, Yasushi; Onozuka, Hiroko; Ochiai, Atsushi; Esumi, Hiroyasu; Soga, Tomoyoshi; Tomita, Masaru

doi:10.1007/s11306-012-0452-2

Cited by 130 publications

(135 citation statements)

References 25 publications

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“…The metabolomic profiles of the tibialis anterior muscles were investigated using capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) performed as previously reported (11,12,16). Briefly, 50 mg of tibialis anterior muscle was transferred to 2,100 mL of 50% (v/v) acetonitrile containing 20 mm of internal standards for cation and 5 mm of internal standards for anion.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we investigated the effect of taurine administration after treadmill running on carbohydrate metabolism in skeletal muscle during the post-exercise phase. First, we identified the levels of metabolites involved in glycogenolysis/glycolysis and the tricarboxylic acid (TCA) cycle and high energy phosphates in skeletal muscle by metabolome analysis using capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) as described in detail previously (11,12). As changes in the maximal enzyme activities and levels of key proteins do not necessarily reflect energy metabolism during the recovery after exercise, we considered it appropriate to use metabolome analysis.…”

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confidence: 99%

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Effects of Taurine Administration on Carbohydrate Metabolism in Skeletal Muscle during the Post-Exercise Phase

Takahashi

Tamura

Matsunaga

et al. 2016

J Nutr Sci Vitaminol

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SummaryWe previously reported that taurine (2-aminoethanesulfonic acid; dose: 0.5 mg/g body weight) administration after treadmill running at 25 m/min for 90 min increased the glycogen concentration in the skeletal muscle of ICR mice at 120 min after the exercise ). In the current study, we further investigated the effects of taurine administration on glycogen repletion and carbohydrate metabolism in the tibialis anterior muscle after endurance exercise. The metabolomic profiles of the tibialis anterior muscle at 120 min after the exercise were analyzed by a capillary electrophoresis-time-offlight mass spectrometry (n56). Fructose-1,6-bisphosphate (F1,6P), a glycogenolytic/glycolytic intermediate produced by phosphofructokinase, was significantly lower in the taurine-treated group than that in the control group (p,0.01). Dihydroxyacetonephosphate (DHAP), a downstream product of F1,6P was lower (p50.05) and glycerol 3-phosphate, a downstream product of F1,6P and DHAP, tended to be lower (p50.09) in the taurinetreated group than in the controls. At that time, phosphorylated Ser 293 on the E1a subunit of pyruvate dehydrogenase (PDH) tended to be higher in the taurine-treated mice than in the controls (p50.09, n55). There was a positive correlation between phosphorylation of the PDH E1a subunit at Ser 293 and glycogen concentration (r50.73, p,0.05). Our results showed that the enhanced glycogen repletion in skeletal muscle by taurine treatment during the post-exercise phase was accompanied by the lower levels of glycogenolytic/glycolytic intermediates. Key Words skeletal muscle, post-exercise recovery, taurine, glycogenolysis/glycolysis, pyruvate dehydrogenase Skeletal muscle glycogen level is considered a major determinant of performance during prolonged moderate-to high-intensity exercise (1-4). When athletes participate in multiple training sessions or competitions, recovery of skeletal muscle glycogen content after exercise is one of the determinants of subsequent exercise performance. As it was reported that oral administration of taurine (2-aminoethanesulfonic acid), which is a sulfur-containing b-amino acid that does not act as a substrate for energy metabolism, increased glycogen concentration in the liver after glucose loading (5), we previously examined whether taurine administration stimulates post-exercise glycogen repletion in skeletal muscle (6). Indeed, we observed enhanced glycogen repletion in skeletal muscle of the taurine-treated mice compared with that of the control mice after endurance exercise. Furthermore, we also found that taurine administration significantly increased voluntary wheel running activity after strenuous exercise in mice; voluntary wheel running activity is thought to indicate recovery from exercise-induced fatigue (7). These results suggest that post-exercise taurine treatment enhanced glycogen repletion in skeletal muscle and then improved performance during subsequent exercise.Taurine is thought to be involved in various physiological processes including antioxidant processe...

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Effects of Taurine Administration on Carbohydrate Metabolism in Skeletal Muscle during the Post-Exercise Phase

Takahashi

Tamura

Matsunaga

et al. 2016

J Nutr Sci Vitaminol

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“…Cancer cells adapt their metabolic pathways to meet the high-energy demands required for their accelerated growth and proliferation and the associated metabolic stresses. Metabolomic studies have revealed that the steady-state abundance of many amino acids in stomach, colon, lung, and prostate cancers is higher than in the corresponding normal tissue, suggesting that the tumors have increased biosynthetic needs for amino acids (6, 7). For example, some tumor cells are addicted to glutamine because it supports anabolic processes and fuels proliferation (8, 9).…”

Section: Introductionmentioning

confidence: 99%

Arginine Starvation Impairs Mitochondrial Respiratory Function in ASS1-Deficient Breast Cancer Cells

et al. 2014

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Autophagy is the principal catabolic response to nutrient starvation and is necessary to clear dysfunctional or damaged organelles, but excessive autophagy can be cytotoxic or cytostatic and contributes to cell death. Depending on the abundance of enzymes involved in molecule biosynthesis, cells can be dependent on uptake of exogenous nutrients to provide these molecules. Argininosuccinate synthetase 1 (ASS1) is a key enzyme in arginine biosynthesis, and its abundance is reduced in many solid tumors, making them sensitive to external arginine depletion. We demonstrated that prolonged arginine starvation by exposure to ADI-PEG20 (pegylated arginine deiminase) induced autophagy-dependent death of ASS1-deficient breast cancer cells, because these cells are arginine auxotrophs (dependent on uptake of extracellular arginine). Indeed, these breast cancer cells died in culture when exposed to ADI-PEG20 or cultured in the absence of arginine. Arginine starvation induced mitochondrial oxidative stress, which impaired mitochondrial bioenergetics and integrity. Furthermore, arginine starvation killed breast cancer cells in vivo and in vitro only if they were autophagy-competent. Thus, a key mechanism underlying the lethality induced by prolonged arginine starvation was the cytotoxic autophagy that occurred in response to mitochondrial damage. Last, ASS1 was either low in abundance or absent in more than 60% of 149 random breast cancer bio-samples, suggesting that patients with such tumors could be candidates for arginine starvation therapy.

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“…However, as mentioned before, the complexity of serum/plasma and urine matrices is a factor to be considered in the search for biomarkers since other pathological conditions could interfere, particularly when a panel of markers formed by metabolites involved in primary metabolism is found. This is the case with amino acids or compounds involved in energy metabolism (glycolysis or Krebs' cycle) [27] as they have shown to be modified in tumor cells. Specifically, potential markers found in urine included modified nucleosides, sarcosine, creatinine, or acetylglutamine [26,28].…”

Section: Introductionmentioning

confidence: 99%

Human sweat metabolomics for lung cancer screening

et al. 2015

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Sweat is one of the less employed biofluids for discovery of markers in spite of its increased application in medicine for detection of drugs or for diagnostic of cystic fibrosis. In this research, human sweat was used as clinical sample to develop a screening tool for lung cancer, which is the carcinogenic disease with the highest mortality rate owing to the advanced stage at which it is usually detected. In this context, a method based on the metabolite analysis of sweat to discriminate between patients with lung cancer versus smokers as control individuals is proposed. The capability of the metabolites identified in sweat to discriminate between both groups of individuals was studied and, among them, a trisaccharide phosphate presented the best independent performance in terms of the specificity/sensitivity pair (80 and 72.7%, respectively). Additionally, two panels of metabolites were configured using the PanelomiX tool as an attempt to reduce false negatives (at least 80% specificity) and false positives (at least 80% sensitivity). The first panel (80% specificity and 69% sensitivity) was composed by suberic acid, a tetrahexose, and a trihexose, while the second panel (69% specificity and 80% sensitivity) included nonanedioic acid, a trihexose, and the monoglyceride MG(22:2). Thus, the combination of the five metabolites led to a single panel providing 80% specificity and 79% sensitivity, reducing the false positive and negative rates to almost 20%. The method was validated by estimation of within-day and between-days variability of the quantitative analysis of the five metabolites

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Metabolomic profiling of lung and prostate tumor tissues by capillary electrophoresis time-of-flight mass spectrometry

Cited by 130 publications

References 25 publications

Effects of Taurine Administration on Carbohydrate Metabolism in Skeletal Muscle during the Post-Exercise Phase

Effects of Taurine Administration on Carbohydrate Metabolism in Skeletal Muscle during the Post-Exercise Phase

Arginine Starvation Impairs Mitochondrial Respiratory Function in ASS1-Deficient Breast Cancer Cells

Human sweat metabolomics for lung cancer screening

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