Metabolomic profiling from leaves and roots of tomato ( Solanum lycopersicum L.) plants grown under nitrogen, phosphorus or potassium-deficient condition

Sung, Jwakyung; Lee, Su-Yeon; Lee, Yejin; Ha, Sang‐Keun; Song, Beom-Heon; Kim, Tae‐Wan; Waters, Brian M.; Krishnan, Hari B.

doi:10.1016/j.plantsci.2015.09.027

Cited by 124 publications

(109 citation statements)

References 78 publications

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“…The results of heat maps of untargeted compounds obtained from 171 in leaf and 227 in root and PCA (principal component analysis) was described in the previous study in detail (Sung et al, 2015), and we here concentrate on explaining and interpreting the results of lipids, nucleotides, peptides and secondary metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…Substantial information on plant metabolic changes in a variety of mineral stress has been provided with various plant species such as Arabidopsis (Hirai et al, 2004;Tschoep et al, 2009), Barlely (Huang et al, 2008), Bean (Hernandez et al, 2007), Gentiana (Takahashi et al, 2012), Spinach (Okazaki et al, 2008), and Tomato (UrbanczykWochniak and Fernie, 2005;Sung et al, 2015). Such efforts enable us to understand how inorganic nutrients are absorbed better and allocated by plants and, finally, metabolized with organic compounds.…”

Section: Introductionmentioning

confidence: 99%

“…However, as a result of being focused on C/N metabolism (glycolysis and TCA cycle), it is necessary to elucidate how metabolites such as nucleotides, peptides, lipids, phenylpropanoids-derivates are regulated by severely low mineral conditions. In the previous study (Sung et al, 2015), we obtained several new findings on C/N (glycolysis and TCA cycle) metabolism in leaf and root of tomato plants grown under NPK-starved conditions. Our hypothesis is that the mineral starvation contributes differently to the metabolic responses with the type of mineral and tissue.…”

Section: Introductionmentioning

confidence: 99%

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Mineral- and Tissue-Specific Metabolic Changes in Tomato (Lycopersicon esculentum L.) Plants Grown under NPK-Starved Conditions

Sung¹,

Lee²,

Lee³

et al. 2016

Korean J. Soil. Sci. Fert.

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Specific metabolic network responses to mineral starvation are not well-defined. We examined a detailed broad-scale identification of metabolic responses of tomato leaf and root to N, P or K starvation. Tomato plants were grown hydroponically under optimal (5 mM N, 0.5 mM P, or 5 mM K) and starved (0.5 mM N, 0.05 mM P, or 0.5 mM K) conditions and metabolites were measured by LC-MS and GC-MS. Overall, the levels of metabolites (lipids, nucleotides, peptides and secondary metabolites) presented in this paper largely showed mineral-and tissue-specific responses. Most strikingly, G3P (glycerol-3-P), GPC (glycerol-P-choline) and choline phosphate responded differently to a type of mineral; an increase in N or K starvation and a decrease in P starvation. A dramatic increase in the levels of secondary metabolites, in particular, rutin and chlorogenate in both tomato tissues during N starvation were observed. Based on these data, it is necessary to clearly elucidate an unknown event taking place in a variety of abiotic impacts, and we are now studying to expand our knowledge on metabolic-and proteomic-responses using GS-MS and LC-MS.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mineral- and Tissue-Specific Metabolic Changes in Tomato (Lycopersicon esculentum L.) Plants Grown under NPK-Starved Conditions

Sung¹,

Lee²,

Lee³

et al. 2016

Korean J. Soil. Sci. Fert.

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“…In particular, CY2 and H5 differentiate from control all roots treated with bioeffectors except B4 for the former and B1 for the latter, which strengthens the hypothesis that the inoculation of plant roots modifies their metabolism. This triggers a decreased response to stress that is ordinarily induced by either a phytopathogen attack or in the absence or scarcity of nutrients [17,18,[24][25][26][27][28]. Other variables in this axis may be seen as markers to differentiate B2 from control (U1 and U2) and from B1 (CY1, L11 and L13) (Fig.…”

Section: Pca Analysismentioning

confidence: 99%

“…While a current consensus holds that phenolic compounds are the main factors involved in adaptability of tomato plants to stress [16,17], it is not yet clear which metabolic pathways are influenced in roots by microorganisms when they act as biostimulants [18].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Nebbioso¹,

Martino

Eltlbany

et al. 2016

Chem. Biol. Technol. Agric.

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Background: In light of the growing interest for eco-compatible fertilization, tomato plant roots were treated with four different strains of microorganisms (B1-B4) capable of positively affecting plant growth. The methanolic extracts from treated roots were analysed by reverse phase ultra-high-performance liquid chromatography hyphenated with ion trap time-of-flight high-resolution mass spectrometry to compare their metabolites with that of control plants (B0). Results:We found, in both treated and control plants, several primary metabolites, such as fatty acids and coumaric acid, and other compounds associated with secondary metabolism pathways such as that of cyclopentaneoctanoic acid (CPOA) or hydroxyoctadecadienoic acid (HODE), and additional molecules which were not characterizable with the available data. A semiquantitative assessment of all metabolites became the basis for further processing the metabolic results by principal component analysis, which highlighted significant differences in the PC1 and PC2 components. The PC1 was particularly affected by the presence of arachidonic acid, myristic acid, and two unidentified metabolites. It effectively differentiated control plants from all bioeffectors treatments, and, in particular, the B4 treatment from the rest (B1-B3). The PC2 was mainly affected by palmitic acid, heptadecanoic acid, two CPOAs, one HODE and two unidentified metabolites. These metabolites successfully differentiated the B0 control from all the bioeffectors treatments, and, especially, showed a difference between B1 and B2. Conclusions:Our findings suggest that changes in secondary pathways of lipid metabolism may underlie the biostimulation exerted by the four microbial bioeffectors of this study, and that LC-MS coupled by multivariate analysis can easily fingerprint the metabolic alterations induced by bioeffectors in tomato roots.

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Advances of Metabolite Profiling of Plants in Challenging Environments

Sarabia

Hill

Boughton

et al. 2018

Annual Plant Reviews Online

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Plant metabolism is profoundly involved in physiological regulation and defence responses when the environment is adverse and plant growth and development is negatively affected. Metabolomics techniques allow the characterisation of physiological and biochemical responses to different types of environmental stresses in plants, including drought, salinity, and nutrient deficiencies. Metabolomics analyses of plant systems have been mostly carried out on bulked tissues (i.e. whole roots, leaves, or shoots) which can provide important biological information about plant tolerance and avoidance mechanisms to abiotic stresses. However, individual plant organs and tissues are composed of different kinds of cells which can produce specific metabolic responses to abiotic stress. Thus, recent development and improvement of metabolomics techniques have allowed the analysis of plant metabolic changes in a spatially resolved manner (i.e.in vivometabolomics, cell‐specific metabolomics, and mass spectrometry imaging (MSI)‐based metabolomics). This article provides a comprehensive overview of the recent findings on plant metabolite changes in response to abiotic stress, recent advancements in metabolomics techniques to study plant metabolism, and prospects of MSI‐based plant metabolomics for the study of plant metabolism.

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Metabolomic profiling from leaves and roots of tomato ( Solanum lycopersicum L.) plants grown under nitrogen, phosphorus or potassium-deficient condition

Cited by 124 publications

References 78 publications

Mineral- and Tissue-Specific Metabolic Changes in Tomato (Lycopersicon esculentum L.) Plants Grown under NPK-Starved Conditions

Mineral- and Tissue-Specific Metabolic Changes in Tomato (Lycopersicon esculentum L.) Plants Grown under NPK-Starved Conditions

Phytochemical profiling of tomato roots following treatments with different microbial inoculants as revealed by IT-TOF mass spectrometry

Advances of Metabolite Profiling of Plants in Challenging Environments

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