Metabolomic profiles illuminate the efficacy of Chinese herbal Da-Cheng-Qi decoction on acute pancreatitis in rats

Li, Juan; Zhu, Shifeng; Zhao, Xiaojing; Liu, Yi-xia; Wan, Mei-Hua; Guo, Hui; Liu, Yiling; Gong, Hanlin; Chen, Guangyuan; Tang, Wen-Fu

doi:10.1016/j.pan.2015.04.011

Cited by 15 publications

(7 citation statements)

References 23 publications

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“…So, regulating the metabolism of amino acids may serve as a potential therapy for SAP-AKI. The previous study has elucidated the capability of Chinese medicine to treat acute pancreatitis by altering the metabolic profile [ 16 ]. As expected, the unique metabolic phenotypes of amino acids in SAP-AKI and after ZYD treatment are presented in the scatter plot of PCA and PLS-DA.…”

Section: Discussionmentioning

confidence: 99%

Zengye Decoction Attenuated Severe Acute Pancreatitis Complicated with Acute Kidney Injury by Modulating the Gut Microbiome and Serum Amino Acid Metabolome

Dai

Yao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Objective. To explore the effect and underlying mechanism of Zengye decoction (ZYD), a traditional formula from China, on the severe acute pancreatitis (SAP) rat model with acute kidney injury (AKI). Methods. The SAP-AKI model was induced by 3.5% sodium taurocholate. Rats were treated with normal saline or ZYD twice and sacrificed at 36 h after modeling. Amylase, lipase, creatinine, blood urea nitrogen, kidney injury molecule 1(KIM-1), and multiple organs’ pathological examinations were used to assess the protective effect of ZYD. Gut microbiome detected by 16S rRNA sequencing analysis and serum amino acid metabolome analyzed by liquid chromatography-mass spectrometry explained the underlying mechanism. The Spearman correlation analysis presented the relationship between microflora and metabolites. Results. ZYD significantly decreased KIM-1( P < 0.05 ) and the pathological score of the pancreas ( P < 0.05 ), colon ( P < 0.05 ), and kidney ( P < 0.05 ). Meanwhile, ZYD shifted the overall gut microbial structure (β-diversity, ANOSIM R = 0.14, P = 0.025 ) and altered the microbial compositions. Notably, ZYD reduced the potentially pathogenic bacteria—Bacteroidetes, Clostridiales vadin BB60 group, and uncultured_Clostridiales_bacterium, but promoted the short-chain fatty acid (SCFA) producers—Erysipelotrichaceae, Bifidobacterium, Lactobacillus, and Moryella (all P < 0.05 ). Moreover, principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA) presented a remarkable change in amino acid metabolome after SAP-AKI induction and an apparent regulation by ZYD treatment (R2Y 0.878, P = 0.01 ; Q2 0.531, P = 0.01 ). Spearman’s correlation analysis suggested that gut bacteria likely influenced serum metabolites levels (absolute r > 0.4 and FDR P < 0.02 ). Conclusions. ZYD attenuated SAP-AKI by modulating the gut microbiome and serum amino acid metabolome, which may be a promising adjuvant treatment.

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Section: Discussionmentioning

confidence: 99%

Zengye Decoction Attenuated Severe Acute Pancreatitis Complicated with Acute Kidney Injury by Modulating the Gut Microbiome and Serum Amino Acid Metabolome

Dai

Yao

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…The severity of AP can be predicted based upon clinical, laboratory, and radiologic risk factors, several score systems, and biomarkers. However, the complex mechanism of AP and its association with a progressive SIRS with high morbidity and mortality is not fully understood, and robust prognostic biomarkers are necessary [ 47 , 48 ]. Unfortunately, none has yet proven to be an excellent accurate predictor of the clinical course of AP.…”

Section: Discussionmentioning

confidence: 99%

Plasmatic Oxidative and Metabonomic Profile of Patients with Different Degrees of Biliary Acute Pancreatitis Severity

et al. 2021

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(1) Background: Acute pancreatitis (AP) is an inflammatory process of the pancreas with variable involvement of the pancreatic and peripancreatic tissues and remote organ systems. The main goal of this study was to evaluate the inflammatory biomarkers, oxidative stress (OS), and plasma metabolome of patients with different degrees of biliary AP severity to improve its prognosis. (2) Methods: Twenty-nine patients with biliary AP and 11 healthy controls were enrolled in this study. We analyzed several inflammatory biomarkers, multifactorial scores, reactive oxygen species (ROS), antioxidants defenses, and the plasma metabolome of biliary AP and healthy controls. (3) Results: Hepcidin (1.00), CRP (0.94), and SIRI (0.87) were the most accurate serological biomarkers of AP severity. OS played a pivotal role in the initial phase of AP, with significant changes in ROS and antioxidant defenses relating to AP severity. Phenylalanine (p < 0.05), threonine (p < 0.05), and lipids (p < 0.01) showed significant changes in AP severity. (4) Conclusions: The role of hepcidin and SIRI were confirmed as new prognostic biomarkers of biliary AP. OS appears to have a role in the onset and progression of the AP process. Overall, this study identified several metabolites that may predict the onset and progression of biliary AP severity, constituting the first metabonomic study in the field of biliary AP.

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“…Another group also reported similar results in 5% sodium taurocholate infusion‐induced SAP, and superior results were reported in combination with emodin (Zhang et al , ). Li et al also reported a role of antiinflammatory activity behind the protection (Li et al , ,b). Moreover, it could effectively check the renal injury induced by SAP.…”

Section: Herbal Remedies Used For Treating Pancreatitismentioning

confidence: 97%

“…Li et al , utilized a novel approach based on metabolomic profile for assessing the efficacy of DTD. They came up with some interesting results clearly showing the efficacy of DTD based on higher levels of plasma glycerol, glutamic acid, low density lipoprotein, saturated fatty acid and lower levels of alanine and glutamine in DTD‐treated rats (Li et al , ,b). This interesting attempt to understand the mechanism of phytoceuticals in SAP can be of great value in the future as it opens up a whole new avenue to shed light on the metabolomics upon treatment with molecule of interest.…”

Section: Herbal Remedies Used For Treating Pancreatitismentioning

confidence: 99%

The Role of Plant-derived Products in Pancreatitis: Experimental and Clinical Evidence

et al. 2017

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Pancreatitis is a gastrointestinal disease with a worldwide sharp surge during the past decade. Pancreatitis includes acute and chronic subtypes, which are graded based on the amount of pancreatic inflammation. Phytoconstituents represent a promising class of therapeutic agents with wide acceptability not only based on folk practices but sound presence of pharmacological and molecular evidences. Growing research evidence indicates that different molecular mechanisms are involved in their protective effect. Many phytoremedies have been tried for the treatment of pancreatic injuries and have shown success in preclinical animal models of pancreatitis. The literature was largely collected through PubMed and Google scholar database. A large proportion of phytochemicals targets the inflammatory cascade and modulates the overtly acting redox balance among which nuclear factor kappa-light-chain-enhancer of activated B cells is the key molecule. Inhibition of apoptosis (artemisinin, embelin), inflammasome (withaferin A), neutrophil rolling (fucoidan), Ca release (caffeine), mitogen activated protein kinase (guggulsterone) and many other novel mechanisms apart from antioxidant effect have been postulated behind the protective effect of phytoconstituents. The present review deals to fill the gap of hitherto unavailable comprehensive review on various plant products screened for the treatment of pancreatitis. The possible mechanistic profile of these phytochemicals is summarized. Copyright © 2017 John Wiley & Sons, Ltd.

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Metabolomic profiles illuminate the efficacy of Chinese herbal Da-Cheng-Qi decoction on acute pancreatitis in rats

Cited by 15 publications

References 23 publications

Zengye Decoction Attenuated Severe Acute Pancreatitis Complicated with Acute Kidney Injury by Modulating the Gut Microbiome and Serum Amino Acid Metabolome

Zengye Decoction Attenuated Severe Acute Pancreatitis Complicated with Acute Kidney Injury by Modulating the Gut Microbiome and Serum Amino Acid Metabolome

Plasmatic Oxidative and Metabonomic Profile of Patients with Different Degrees of Biliary Acute Pancreatitis Severity

The Role of Plant-derived Products in Pancreatitis: Experimental and Clinical Evidence

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