Metabolomic profile and in vitro evaluation of the cytotoxic activity of Asphodelus microcarpus against human malignant melanoma cells A375

Abosedera, Dalia; Emara, S.A.; Tamam, Omar A. S.; Badr, Osama M.; Khalifa, Shaden A. M.; El‐Seedi, Hesham R.; Refaey, Mohamed S.

doi:10.1016/j.arabjc.2022.104174

Cited by 10 publications

(6 citation statements)

References 42 publications

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“…It was determined that the obtained fractions had an effect IC 50 value between 83-65 µg/ml and were suitable for further in vivo studies [25]. In another study, the migration and invasive ability of breast cancer cells was examined and it was observed that it inhibited its metastatic feature [26]. A tenuifolius has been shown to inhibit cell proliferation in melanoma cancer cells [27].…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

Taştan¹,

Fafal²,

TÜZÜN³

et al. 2023

jrp

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The aim of the study is to examine the cytotoxic, apoptotic effects and gene expressions of Asphodelus aestivus water extract (ASP) and silver nanoparticles (AgNPS) on decided cancer lines. Breast cancer cell lines MCF-7 and MDA-231; melanoma cancer lines MEWO and CHL-1; fibroblast cancer lines WI-38 and HEL 299 were selected for biological activities. xCELLigence system was used for cytotoxicity. Annexin V-EG FP Apoptosis detection kit was used for apoptosis and gene expressions were assessed by real time online RT-PCR by using cancer cell lines Qiagen kits. AgNPS showed significant cytotoxicity in all cell lines. The most prominent apoptosis was determined in MCF-7 cell line for AgNPS. It has been observed that the most important progress in gene expression, the suppression capacity of MUC1-C, a breast cancer-associated oncoprotein, is greatly increased by nanoparticle formation. In addition, cells that were not affected at all in MDA-MB-231 breast cancer oncoprotein started to suppress with nanoparticles. In conclusion, it was determined that silver nanoparticles increased the effect especially in breast cancer cell lines and could be considered for use.

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Section: Discussionmentioning

confidence: 99%

“…Plant extract was prepared by 5% infusion method by using the aerial parts of the plant. Infusion was then filtered and solvent was evaporated using vacuum evaporator [26].…”

Section: Preparation Of Plant Extractmentioning

confidence: 99%

Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

Taştan¹,

Fafal²,

TÜZÜN³

et al. 2023

jrp

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“…Extracts of P and RJ were analyzed using Linear Ion Trap-ultra-performance liquid chromatography-tandem mass spectrometry (LTQ-UPLC-MS/MS) as described by Abosedera et al [ 34 ]. A Shimadzu LC-10 HPLC with a Grace Vydac Everest Narrowbore C18 column (100 mm × 2.1 mm i.d., 5 µm, 300 Å).…”

Section: Methodsmentioning

confidence: 99%

UPLC-MS/MS Analysis of Naturally Derived Apis mellifera Products and Their Promising Effects against Cadmium-Induced Adverse Effects in Female Rats

et al. 2022

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Honeybee products arouse interest in society due to their natural origin and range of important biological properties. Propolis (P) and royal jelly (RJ) attract scientists’ attention because they exhibit antioxidant, anti-inflammatory, anti-bacterial, anti-tumor, and immunomodulatory abilities. In this study, we tested whether P and RJ could mitigate the adverse effects of cadmium (Cd) exposure, with particular emphasis on the reproductive function in female rats. In this line, one week of pretreatment was established. Six experimental groups were created, including (i) the control group (without any supplementation), (ii) the Cd group (receiving CdCl2 in a dose of 4.5 mg/kg/day), (iii) the P group (50 mg of P/kg/day), (iv) RJ group (200 mg of RJ/kg/day), (v) P + Cd group (rats pretreated with P and then treated with P and Cd simultaneously), (vi) RJ + Cd group (animals pretreated with RJ before receiving CdCl2 simultaneously with RJ). Cd treatment of rats adversely affected a number of measured parameters, including body weight, ovarian structure and ultrastructure, oxidative stress parameters, increased ovarian Cd content and prolonged the estrous cycle. Pretreatment and then cotreatment with P or RJ and Cd alleviated the adverse effects of Cd, transferring the clusters in the PCA analysis chart toward the control group. However, clusters for cotreated groups were still distinctly separated from the control and P, or RJ alone treated groups. Most likely, investigated honeybee products can alter Cd absorption in the gut and/or increase its excretion through the kidneys and/or mitigate oxidative stress by various components. Undoubtedly, pretreatment with P or RJ can effectively prepare the organism to overcome harmful insults. Although the chemical composition of RJ and P is relatively well known, focusing on proportion, duration, and scheme of treatment, as well as the effects of particular components, may provide interesting data in the future. In the era of returning to natural products, both P and RJ seem valuable materials for further consideration as anti-infertility agents.

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“…Others have anticancer potential in vivo and in vitro [18][19][20]. In continuation of our project to identify new, pharmacologically active sources from Egyptian medicinal plants [21][22][23][24], we investigated, for the first time, the flavonoid-rich methanolic extract of A. hierochuntica. Additionally, we performed detailed molecular networking through the LC-ESI-MS platform and the presence of chemical classes was confirmed by 1 H-NMR.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Induction of Apoptosis in Isolated Acute Myeloid Leukemia Cells: The Role of Anastatica hierochuntica Methanolic Extract

et al. 2022

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Anastatica hierochuntica L. (Cruciferae) has been known in Egyptian folk medicine as a remedy for gastrointestinal disorders, diabetes and heart diseases. Despite the wide usage, A. hierochuntica research provides insufficient data to support its traditional practice. The cytotoxicity of A. hierochuntica methanolic extract was investigated on acute myeloid leukemia blasts (AML) and normal human peripheral leucocytes (NHPL). The phytochemical identification of bioactive compounds using 1H-NMR and LC-ESI-MS was also performed. A. hierochuntica extract caused non-significant cytotoxicity on NHPL, while the cytotoxicity on AML was significant (IC50: 0.38±0.02 μg/mL). The negative expression of p53, upregulation of Caspase-3 and increase in the BAX/BCL-2 ratio were reported at the protein and mRNA levels. The results suggest that A. hierochuntica extract induced AML cell death via the p53-independent mitochondrial intrinsic pathway and further attention should be paid to this plant as a promising natural anticancer agent.

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Metabolomic profile and in vitro evaluation of the cytotoxic activity of Asphodelus microcarpus against human malignant melanoma cells A375

Cited by 10 publications

References 42 publications

Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

Cytotoxic effects of silver nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

UPLC-MS/MS Analysis of Naturally Derived Apis mellifera Products and Their Promising Effects against Cadmium-Induced Adverse Effects in Female Rats

In Vitro Induction of Apoptosis in Isolated Acute Myeloid Leukemia Cells: The Role of Anastatica hierochuntica Methanolic Extract

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