Metabolomic fingerprint in patients at high risk of cardiovascular disease by cocoa intervention

Llorach, Rafaël; Urpí-Sardà, Mireia; Tulipani, Sara; Garcia-Aloy, Mar; Monagas, Marı́a; Andrés‐Lacueva, Cristina

doi:10.1002/mnfr.201200736

Cited by 46 publications

(53 citation statements)

References 64 publications

(86 reference statements)

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“…Based on their exact masses, retention times and in-source fragmentation patterns, these compounds were annotated as belonging to first-stage flavan-3-ol microbial metabolism 57 derivatives of hydroxy-(dihydroxyphenyl)-valeric acid (two glucuronides and sulphate), and derivatives of dihydroxyphenyl-(two glucuronides), hydroxy-methoxyphenyl-(two glucuronides) and hydroxyphenyl-valerolactones (one glucuronide), respectively. Earlier, various phase II hydroxyvaleric acid and hydroxyvalerolactone derivatives, including those identified in the present study, were also reported to be markers of consumption of flavanolrich food such as almond skin, 55 cocoa 26,27 and tea. 52,58 Although grapes, especially red ones, are known to be very rich in flavanols, so far no studies have reported on their microbial metabolites in relation to grape polyphenol consumption.…”

Section: Discussionsupporting

confidence: 72%

“…The increase of epicatechin in urinary host metabolites during both acute and sustained consumption of FB, was obviously related to the intake of flavan-3-ols, which are the most abundant class of flavonoids present in grape and related products. 46,53 In general, epicatechin phase II metabolites are recognized markers of consumption of flavanol-rich food, 26,27,55,56 especially for postprandial stages. Their relation to the consumption of grape and derived food products was recently reported by our group in a targeted metabolomics study on the consumption of dealcoholized wine.…”

Section: Discussionmentioning

confidence: 99%

“…[25][26][27] HPLC-MS analysis was performed using an Agilent 1200 Series Rapid Resolution HPLC system coupled to a hybrid quadrupole TOF QSTAR Elite (Applied Biosystems/MDS Sciex) according to the protocol for untargeted metabolomic analysis established in our group. [25][26][27] Thus, chromatographic separation was performed using a RP 18 Luna 5 µm, 50 × 2.0 mm column (Phenomenex, Torrance, CA) in mobile phases consisted of (A) 0.1% HCOOH and (B) ACN 0.1% HCOOH, at a flow rate of 600 µL min −1 with the following linear gradient elution (t, %B): (0, 1), (4,20), (6,95), (7.5, 95), (8,1), (12,1). q-TOF spray parameters were set as previously described 27 and full data acquisition was performed in the scan range 70-700 m/z in positive ionization mode.…”

Section: Lc-ms Metabolomics Analysismentioning

confidence: 99%

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Metabolic fingerprint after acute and under sustained consumption of a functional beverage based on grape skin extract in healthy human subjects

et al. 2015

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Grape-derived polyphenols are considered to be one of the most promising ingredients for functional foods due to their health-promoting activities. We applied a HPLC-MS-based untargeted metabolomic approach in order to evaluate the impact of a functional food based on grape skin polyphenols on the urinary metabolome of healthy subjects. Thirty-one volunteers participated in two dietary crossover randomized intervention studies: with a single-dose intake (187 mL) and with a 15-day sustained consumption (twice per day, 187 mL per day in total) of a functional beverage (FB). Postprandial (4-hour) and 24-hour urine samples collected after acute consumption and on the last day of sustained FB consumption, respectively, were analysed using an untargeted HPLC-qTOF-MS approach. Multivariate modelling with subsequent application of an S-plot revealed differential mass features related to acute and prolonged consumption of FB. More than half of the mass features were shared between the two types of samples, among which several phase II metabolites of grape-derived polyphenols were identified at confidence level II. Prolonged consumption of FB was specifically reflected in urine metabolome by the presence of first-stage microbial metabolites of flavanols: hydroxyvaleric acid and hydroxyvalerolactone derivatives.Overall, several epicatechin and phenolic acid metabolites both of tissular and microbiota origin were the most representative markers of FB consumption. To our knowledge, this is one of the first studies where an untargeted LC-MS metabolomic approach has been applied in nutrition research on a grapederived FB.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Lc-ms Metabolomics Analysismentioning

confidence: 99%

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Metabolic fingerprint after acute and under sustained consumption of a functional beverage based on grape skin extract in healthy human subjects

et al. 2015

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“…Hence, metabolomics can answer questions such as how a high-saturated fat diet can affect lipid profile, or how the intake of fiber affects glycemia. Recent dietary intervention studies using metabolic profiles have evaluated the consequences of consuming cocoa [96], coffee [97] and fiber [98], and of different dietary patterns [99]. …”

Section: Omics Technologies In Personalized Nutritionmentioning

confidence: 99%

Guide and Position of the International Society of Nutrigenetics/Nutrigenomics on Personalised Nutrition: Part 1 - Fields of Precision Nutrition

et al. 2016

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Diversity in the genetic profile between individuals and specific ethnic groups affects nutrient requirements, metabolism and response to nutritional and dietary interventions. Indeed, individuals respond differently to lifestyle interventions (diet, physical activity, smoking, etc.). The sequencing of the human genome and subsequent increased knowledge regarding human genetic variation is contributing to the emergence of personalized nutrition. These advances in genetic science are raising numerous questions regarding the mode that precision nutrition can contribute solutions to emerging problems in public health, by reducing the risk and prevalence of nutrition-related diseases. Current views on personalized nutrition encompass omics technologies (nutrigenomics, transcriptomics, epigenomics, foodomics, metabolomics, metagenomics, etc.), functional food development and challenges related to legal and ethical aspects, application in clinical practice, and population scope, in terms of guidelines and epidemiological factors. In this context, precision nutrition can be considered as occurring at three levels: (1) conventional nutrition based on general guidelines for population groups by age, gender and social determinants; (2) individualized nutrition that adds phenotypic information about the person's current nutritional status (e.g. anthropometry, biochemical and metabolic analysis, physical activity, among others), and (3) genotype-directed nutrition based on rare or common gene variation. Research and appropriate translation into medical practice and dietary recommendations must be based on a solid foundation of knowledge derived from studies on nutrigenetics and nutrigenomics. A scientific society, such as the International Society of Nutrigenetics/Nutrigenomics (ISNN), internationally devoted to the study of nutrigenetics/nutrigenomics, can indeed serve the commendable roles of (1) promoting science and favoring scientific communication and (2) permanently working as a ‘clearing house' to prevent disqualifying logical jumps, correct or stop unwarranted claims, and prevent the creation of unwarranted expectations in patients and in the general public. In this statement, we are focusing on the scientific aspects of disciplines covering nutrigenetics and nutrigenomics issues. Genetic screening and the ethical, legal, social and economic aspects will be dealt with in subsequent statements of the Society.

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“…The compounds that were increased after the intervention included mainly cocoa-derived metabolites such as polyphenolic metabolites and diketopiperazines. Additionally, some endogenous compounds were increased in the intervention, e.g., metabolites linked with carnitine metabolism [41].…”

Section: Dietary Interventions With Selected Foodsmentioning

confidence: 99%

Application of Metabolomics to Assess Effects of Controlled Dietary Interventions

Hanhineva

2015

Curr Nutr Rep

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The utilization of metabolomics technologies as part of nutritional investigations has rapidly increased during the last few years. The application of both nuclear magnetic resonance (NMR) and mass spectrometry (MS) based technologies is providing wide information on both the dietderived compounds and their metabolites related to specific foods or diets, as well as aiding the in-depth exploration of the endogenous metabolic phenomena related to different diets. The analytical accuracy nowadays enables examination of metabolite composition of both urine and plasma to very high molecular detail even at low concentrations, and will be focal in gaining wider metabolic understanding of the relationship between eating habits and maintenance of good health.

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Metabolomic fingerprint in patients at high risk of cardiovascular disease by cocoa intervention

Abstract: LC-MS metabolomics strategy allows the defining of a complex metabolic profile derived from cocoa phytochemicals. Likewise, the identification of endogenous markers could lead to new hypotheses to unravel the relationship between cocoa intake and cardiovascular diseases.

Cited by 46 publications

References 64 publications

Metabolic fingerprint after acute and under sustained consumption of a functional beverage based on grape skin extract in healthy human subjects

Metabolic fingerprint after acute and under sustained consumption of a functional beverage based on grape skin extract in healthy human subjects

Guide and Position of the International Society of Nutrigenetics/Nutrigenomics on Personalised Nutrition: Part 1 - Fields of Precision Nutrition

Application of Metabolomics to Assess Effects of Controlled Dietary Interventions

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