Metabolic fingerprint after acute and under sustained consumption of a functional beverage based on grape skin extract in healthy human subjects

Abstract

Grape-derived polyphenols are considered to be one of the most promising ingredients for functional foods due to their health-promoting activities. We applied a HPLC-MS-based untargeted metabolomic approach in order to evaluate the impact of a functional food based on grape skin polyphenols on the urinary metabolome of healthy subjects. Thirty-one volunteers participated in two dietary crossover randomized intervention studies: with a single-dose intake (187 mL) and with a 15-day sustained consumption (twice per day, 187 mL per day in total) of a functional beverage (FB). Postprandial (4-hour) and 24-hour urine samples collected after acute consumption and on the last day of sustained FB consumption, respectively, were analysed using an untargeted HPLC-qTOF-MS approach. Multivariate modelling with subsequent application of an S-plot revealed differential mass features related to acute and prolonged consumption of FB. More than half of the mass features were shared between the two types of samples, among which several phase II metabolites of grape-derived polyphenols were identified at confidence level II. Prolonged consumption of FB was specifically reflected in urine metabolome by the presence of first-stage microbial metabolites of flavanols: hydroxyvaleric acid and hydroxyvalerolactone derivatives.Overall, several epicatechin and phenolic acid metabolites both of tissular and microbiota origin were the most representative markers of FB consumption. To our knowledge, this is one of the first studies where an untargeted LC-MS metabolomic approach has been applied in nutrition research on a grapederived FB.

The acute impact of polyphenols from Hibiscus sabdariffa in metabolic homeostasis: an approach combining metabolomics and gene-expression analyses

“…7,15,[22][23][24][54][55][56][57] Tissue-specific transcriptomic information requires invasive procedures, and whether changes in PBMCs are indicative of the metabolism in other tissues, although suggestive, needs confirmation. Inferring the routes of interacting biological macromolecules may be considered as a promising and complementary tool for capturing the metabolic complexity of phytochemical exposure.…”

“…16 The present study was performed under the rationale that the chemical composition and conformational changes of dietary polyphenols are responsible for binding to different metabolically active enzymes and/or receptors, and consequently may have the inherent potential to exert multiple effects. [22][23][24] 2. 20 To reduce the complexity found in the composition of plant foods in common diets, which include hundreds of polyphenols, 21 we studied the acute effects of a polyphenol-rich, fully characterized aqueous extract from the calyces of Hibiscus sabdariffa Linnaeus (Malvaceae) (HS).…”

Metabolic faecal fingerprinting of trans-resveratrol and quercetin following a high-fat sucrose dietary model using liquid chromatography coupled to high-resolution mass spectrometry

“…7 In this sense, metabolomics has been suggested as a high-throughput approach that performs a comprehensive analysis of the metabolome, defined as the collection of low molecular weight molecules produced by cells 8 and has become a promising diagnostic tool for metabolic arrangement of individuals. 9,10 Metabolomic characterization provides the potential to distinguish biomarkers and contribute to the knowledge of the ethio-pathological processes, 11 allowing discovery new targets and tools to be applied in personalized therapies. 12 Importantly, the role of gut microbiota in the conversion of phytochemicals should not be disregarded.…”

Food intake biomarkers for berries and grapes

“…From the 880 unique citations screened, 32 original papers fulfilled the inclusion criteria of this review (Fig. 1 ) [ 6 , 15 , 19 , 20 , 23 , 24 , 29 , 33 , 55 , 66 , 69 , 71 , 79 , 84 , 85 , 96 , 97 , 99 , 107 , 117 , 118 , 121 , 122 , 131 , 138 , 142 , 143 , 149 , 150 , 157 , 158 , 168 , 171 , 177 ]. The grape extract was included in this review, considering that elimination of water fraction during desiccation does not drastically change the overall polyphenol content.…”