Metabolic faecal fingerprinting of trans-resveratrol and quercetin following a high-fat sucrose dietary model using liquid chromatography coupled to high-resolution mass spectrometry

Etxeberría, Usune; Arias, N.; Boqué, Noemí; Romo-Hualde, Ana; Macarulla, M. T.; Portillo, Marı́a P.; Milagro, Fermı́n I.; Martínéz, J. Alfredo

doi:10.1039/c5fo00473j

Cited by 25 publications

(8 citation statements)

References 44 publications

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“…S4), indicating that after polyphenol intervention, the content of aminophospholipids in the liver of rats fed with high-fat diet was significantly increased, which was closer to that in the livers of normal rats. Rapeseed polyphenols and grape seed polyphenols have a role in improvement of metabolic diseases, such as nonalcoholic fatty liver disease and cardiovascular disease (19), which are consistent with the results of this study. Furthermore, by comparison of the polyphenol intervention groups S, R, and F, it was found that the increase of PE and PS content in the R group was the most obvious (supplemental Fig.…”

Section: Quantification Of Aminophospholipid Species Present In Rat Lsupporting

confidence: 92%

Profiling and quantification of aminophospholipids based on chemical derivatization coupled with HPLC-MS

Wei

et al. 2019

Journal of Lipid Research

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and metabolic roles (1). PLs consist of a glycerol backbone esterified with two fatty acids at the sn-1 and sn-2 positions. The backbone's sn-3 position is occupied by a phosphate group attached to a polar head of different nature. Aminophospholipids [phosphatidylethanolamine (PE) and phosphatidylserine (PS)] belong to the category of PLs. PE and PS account for approximately 20% and 3-15% of total PLs in eukaryotic cells, respectively. They play a critical role in regulating various biological processes, including intraand inter-cellular signaling, phagocytic recognition, apoptotic cell clearance, cell division, angiogenesis, and vascular remodeling under normal physiological conditions in eukaryotic cells (2). Furthermore, aminophospholipids are associated with various human diseases, including cancer, diabetes, obesity, and neurodegeneration (3, 4). Hence, profiling of the individual aminophospholipid molecular species in cellular components is critical to link physiological or pathophysiological changes to the underlying biochemistry and thus target therapeutic interventions. However, precise profiling of aminophospholipids is not easy because of the sheer number and the high complexities of their structures. Direct infusion MS equipped with an ESI source holds much promise for the characterization of PLs (5). Through effective intra-source separation of predetermined groups of lipid classes according to their intrinsic electrical propensities, lipid extracts can be analyzed directly without preseparation. However, the disadvantages of the direct infusion MS method are ion suppression for lipid species with low proton affinities, negative impact of unavoidable matrix effects on the analysis of minor content of lipid species in biological samples, and serious contamination to Abstract In this study, a novel strategy based on acetone stable-isotope derivatization coupled with HPLC-MS for profiling and accurate quantification of aminophospholipids (phosphatidylethanolamine and phosphatidylserine) in biological samples was developed. Acetone derivatization leads to alkylation of the primary amino groups of aminophospholipids with an isopropyl moiety; the use of deuterium-labeled acetone (d6-acetone) introduced a 6 Da mass shift that was ideally suited for profiling and quantification analysis with high selectivity and accuracy. After derivatization, significantly increased column efficiency for chromatographic separation and detection sensitivity for MS analysis of aminophospholipids was observed. Furthermore, an accuracy quantification method was developed. Aminophospholipids in biological samples were derivatized with d0-acetone; while more than two aminophospholipid standards were selected for each class of aminophospholipid and derivatized with d6-acetone, which were then used as the internal standards to typically construct a calibration curve for each class to normalize the nonuniformity response caused by the differential fragmentation kinetics resulting from the distinct chemical constitution of individual...

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Section: Quantification Of Aminophospholipid Species Present In Rat Lsupporting

confidence: 92%

Profiling and quantification of aminophospholipids based on chemical derivatization coupled with HPLC-MS

Wei

et al. 2019

Journal of Lipid Research

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“…The microbial metabolites of resveratrol include dihydroresveratrol, piceid and lunularin in human, rat and in vitro studies (Jung et al , 2009; Bode et al , 2013; Etxeberria et al , 2015). However, we were only able to detect dihydroresveratrol in the in vitro fermentation system.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol affects in vitro rumen fermentation, methane production and prokaryotic community composition in a time‐ and diet‐specific manner

Yan

et al. 2020

Microbial Biotechnology

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This study aimed to investigate the effect of resveratrol on methane production, rumen fermentation and microbial composition under high-concentrate (HC) and high-forage (HF) diets using the in vitro fermentation system. A total of 25 mg of resveratrol was supplemented into 300 mg of either HC or HF diet. Methane production, total volatile fatty acid (VFA) concentration, molar proportion of VFA, metabolites of resveratrol and prokaryotic community composition were measured after 12 and 24 h of in vitro fermentation. Resveratrol reduced methane production (ml per mg of dry matter degraded) by 41% and 60% under both HC and HF diets (P < 0.001), respectively, and this result could be associated with the lower abundance of Methanobrevibacter (P < 0.001) in response to resveratrol. The molar proportion of propionate was significantly higher in the resveratrol group only under the HC diet (P = 0.045). The relative abundance of 10 bacterial genera was affected by the three-way interaction of treatment, diet and time (P < 0.05). Resveratrol was partly converted to dihydroresveratrol after 24 h of fermentation, and its degradation could be associated with microbes belonging to the order Coriobacteriales. Our results suggest that multiple factors (e.g. diet and time) should be considered in animal experiments to test the effect of polyphenol or other plant extracts on rumen fermentation, methane emission and microbial composition.

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“…Metabolomics have been applied for pattern recognition and characteristic metabolite identification [ 40 , 41 ], as well as for dietary adherence [ 27 ] metabolic fingerprinting [ 42 ], disease monitoring [ 43 ], and post-treatment outcomes [ 44 ]. Untargeted metabolomic analyses of urine samples collected at baseline and at the endpoint in a nutritional intervention might offer information on the presence of discriminant metabolites among experimental groups, since metabolomics has contributed to decipher body responses to different treatments in subjects with obesity [ 45 ], diabetes [ 46 ], fatty liver [ 47 ] and COPD [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Untargeted metabolomic on urine samples after α-lipoic acid and/or eicosapentaenoic acid supplementation in healthy overweight/obese women

et al. 2018

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BackgroundEicosapentaenoic acid (EPA) and α-lipoic acid (α-LA) have been investigated for their beneficial effects on obesity and cardiovascular risk factors. In the current research, the goal was to evaluate metabolomic changes following the dietary supplementation of these two lipids, alone or combined in healthy overweight/obese sedentary women following an energy-restricted diet. For this purpose, an untargeted metabolomics approach was conducted on urine samples using liquid chromatography coupled with time of flight mass spectrometry (HPLC-TOF-MS).MethodsThis is a short-term double blind placebo-controlled study with a parallel nutritional design that lasted 10 weeks. Participants were assigned to one of the 4 experimental groups [Control, EPA (1.3 g/d), α-LA (0.3 g/d) and EPA+α-LA (1.3 g/d + 0.3 g/d)]. All intervention groups followed an energy-restricted diet of 30% less than total energy expenditure. Clinically relevant biochemical measurements were analyzed. Urine samples (24 h) were collected at baseline and after 10 weeks. Untargeted metabolomic analysis on urine samples was carried out, and principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were performed for the pattern recognition and characteristic metabolites identification.ResultsUrine samples were scattered in the PCA scores plots in response to the supplementation with α-LA. Totally, 28 putative discriminant metabolites in positive ionization, and 6 in negative ionization were identified among groups clearly differentiated according to the α-LA administration. Remarkably is the presence of an ascorbate intermediate metabolite (one of the isomers of trihydroxy-dioxohexanoate, or dihydroxy–oxohexanedionate) in the groups supplemented with α-LA. This fact might be associated with antioxidant properties of both α-LA and ascorbic acid. Correlations between phenotypical parameters and putative metabolites of provided additional information on whether there is a direct or inverse relationship between them. Especially interesting are the negative correlation between ascorbate intermediate metabolite and asymmetric dimethylarginine (ADMA) and the positive one between superoxide dismutase (SOD) and α-LA supplementation.ConclusionsThis metabolomic approach supports that the beneficial effects of α-LA administration on body weight reduction may be partly explained by the antioxidant properties of this organosulfur carboxylic acid mediated by isomers of trihydroxy-dioxohexanoate, or dihydroxy–oxohexanedionate.Trial registrationClinicaltrials.gov NCT01138774.Electronic supplementary materialThe online version of this article (10.1186/s12944-018-0750-4) contains supplementary material, which is available to authorized users.

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Metabolic faecal fingerprinting of trans-resveratrol and quercetin following a high-fat sucrose dietary model using liquid chromatography coupled to high-resolution mass spectrometry

Cited by 25 publications

References 44 publications

Profiling and quantification of aminophospholipids based on chemical derivatization coupled with HPLC-MS

Profiling and quantification of aminophospholipids based on chemical derivatization coupled with HPLC-MS

Resveratrol affects in vitro rumen fermentation, methane production and prokaryotic community composition in a time‐ and diet‐specific manner

Untargeted metabolomic on urine samples after α-lipoic acid and/or eicosapentaenoic acid supplementation in healthy overweight/obese women

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