Metabolomic disorders unveil hepatotoxicity of environmental microplastics in wild fish Serranus scriba (Linnaeus 1758)

Zitouni, Nesrine; Cappello, Tiziana; Missawi, Omayma; Boughattas, Iteb; Belbekhouche, Sabrina; Mokni, Moncef; Alphonse, Vanessa; Guerbej, Hamadi; Bousserrhıne, Noureddine; Bannı, Mohamed

doi:10.1016/j.scitotenv.2022.155872

Cited by 33 publications

(11 citation statements)

References 84 publications

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“…To date, the use of metabolomics has proven to be an extremely effective tool for investigations of the perturbed metabolic pathways in aquatic biota triggered by a variety of pollutants [ 26 , 31 , 32 , 49 , 50 , 51 ], including Hg [ 20 , 40 , 41 ]. In this work, the metabolomic approach was applied on gills and liver of white seabream, D. sargus , with the aim of elucidating the cytotoxic effects of dietary MeHg and the potential recovery ability of the selected target organs during a successive post-exposure period, revealing differential impairments in various metabolic pathways.…”

Section: Discussionmentioning

confidence: 99%

“…Although Hg toxicity has been the topic of several studies [ 17 , 18 , 19 ], clarifying the mechanisms of dietary MeHg toxicity in fish remains challenging. Fish have been widely used in ecotoxicological studies as worthy sentinel organisms [ 6 , 20 , 21 , 22 , 23 , 24 , 25 , 26 ] due to their ability to accumulate and metabolize different contaminants in their tissues and to produce valid and measurable responses (biomarkers) when exposed to stressful conditions, such as the presence of Hg.…”

Section: Introductionmentioning

confidence: 99%

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Differential Cell Metabolic Pathways in Gills and Liver of Fish (White Seabream Diplodus sargus) Coping with Dietary Methylmercury Exposure

Billè

Brandão

Galati

et al. 2023

Toxics

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Mercury (Hg) is a dangerous and persistent trace element. Its organic and highly toxic form, methylmercury (MeHg), easily crosses biological membranes and accumulates in biota. Nevertheless, understanding the mechanisms of dietary MeHg toxicity in fish remains a challenge. A time-course experiment was conducted with juvenile white seabreams, Diplodus sargus (Linnaeus, 1758), exposed to realistic levels of MeHg in feed (8.7 μg g−1, dry weight), comprising exposure (E; 7 and 14 days) and post-exposure (PE; 28 days) periods. Total Hg levels increased with time in gills and liver during E and decreased significantly in PE (though levels of control fish were reached only for gills), with liver exhibiting higher levels (2.7 times) than gills. Nuclear magnetic resonance (NMR)-based metabolomics revealed multiple and often differential metabolic changes between fish organs. Gills exhibited protein catabolism, disturbances in cholinergic neurotransmission, and changes in osmoregulation and lipid and energy metabolism. However, dietary MeHg exposure provoked altered protein metabolism in the liver with decreased amino acids, likely for activation of defensive strategies. PE allowed for the partial recovery of both organs, even if with occurrence of oxidative stress and changes of energy metabolism. Overall, these findings support organ-specific responses according to their sensitivity to Hg exposure, pointing out that indications obtained in biomonitoring studies may depend also on the selected organ.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential Cell Metabolic Pathways in Gills and Liver of Fish (White Seabream Diplodus sargus) Coping with Dietary Methylmercury Exposure

Billè

Brandão

Galati

et al. 2023

Toxics

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“…Furthermore, based on the histological observations, we also found a recovery tendency from CuO-NP-induced tissue damage in the gills, livers, and kidneys of common carp in a dose-dependent manner after treatment with the fenugreek extract. The liver is very important organ that performs vital functions including detoxification, synthesis of numerous components of blood plasma, storage of glycogen, and release of glucose to the blood [ 83 , 84 , 85 , 86 ]; gills are crucial for respiratory gas exchange activity, osmoregulation, active ion transport, acid–base balance and excretion of nitrogenous wastes [ 84 , 85 ]; whereas kidneys are the primary excretory and osmoregulatory organ in fish [ 87 ]. Therefore, the maintenance of the histological stability and integrity of these organs is critical for proper nutrition, defense, and fish growth.…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Hematological and Histomorphological Effects of Dietary Trigonella foenum-graecum Seeds in Common Carp (Cyprinus carpio) Exposed to Copper Oxide Nanoparticles

Noureen

Rehman

Jabeen

et al. 2022

IJERPH

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Different types of metal oxide nanoparticles (NPs) are being used for wastewater treatment worldwide but concerns have been raised regarding their potential toxicities, especially toward non-targeted aquatic organisms including fishes. Therefore, the present study aimed to evaluate the toxicity of copper oxide (CuO) NPs (1.5 mg/L; positive control group) in a total of 130 common carp (Cyprinus carpio), as well as the potential ameliorative effects of fenugreek (Trigonella foenum-graecum) seed extracts (100 mg/L as G-1 group, 125 mg/L as G-2 group, and 150 mg/L as G-3 group) administered to fish for 28 days. Significant changes were observed in the morphometric parameters: the body weight and length of the CuO-NP-treated fish respectively decreased from 45.28 ± 0.34 g and 14.40 ± 0.56 cm at day one to 43.75 ± 0.41 g and 13.57 ± 0.67 cm at day 28. Conversely, fish treated with T. foenum-graecum seed extract showed significant improvements in body weight and length. After exposure to CuO NPs, a significant accumulation of Cu was recorded in the gills, livers, and kidneys (1.18 ± 0.006 µg/kg ww, 1.38 ± 0.006 µg/kg ww, and 0.05 ± 0.006 µg/kg ww, respectively) of the exposed common carp, and significant alterations in fish hematological parameters and oxidative stress biomarkers (lipid peroxidation (LPO), glutathione (GSH), and catalase (CAT)) were also observed. However, supplementing diets with fenugreek extracts modulated the blood parameters and the oxidative stress enzymes. Similarly, histological observations revealed that sub-lethal exposure to CuO NPs caused severe histomorphological changes in fish gills (i.e., degenerative epithelium, fused lamellae, necrotic lamellae, necrosis of primary lamellae, complete degeneration, and complete lamellar fusion), liver (i.e., degenerative hepatocytes, vacuolization, damaged central vein, dilated sinusoid, vacuolated degeneration, and complete degeneration), and kidney (i.e., necrosis and tubular degeneration, abnormal glomerulus, swollen tubules, and complete degeneration), while the treatment with the fenugreek extract significantly decreased tissue damage in a dose-dependent manner by lowering the accumulation of Cu in the selected fish tissues. Overall, this work demonstrated the ameliorative effects of dietary supplementation with T. foenum-graecum seed extract against the toxicity of NPs in aquatic organisms. The findings of this study therefore provided evidence of the promising nutraceutical value of fenugreek and enhanced its applicative potential in the sector of fish aquaculture, as it was shown to improve the growth performance and wellness of organisms.

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“…The study recorded 22–100% and 22–89% MPs from the gills and guts of the tested fishes, respectively; however, there were no MPs found in their liver and muscle except for a few contaminants [ 48 ]. Zitouni et al [ 50 ] reported MPs (2.90 ± 1.47 MP/g) contamination in the tissues of the edible commercial fish, Serranus scriba , caught from the coastal waters of Tunisia. Furthermore, commercial fishes such as Sarpa salpa and Liza aurata from the lagoons of Bizerte and Ghar El Melh in Tunisia were detected for MPs in their stomach in Ref.…”

Section: Comprehensive Overview Of Mp Impacts On Edible Marine and Fr...mentioning

confidence: 99%

Overviewing the Ground Reality of Microplastic Effects on Seafoods, Including Fish, Shrimps and Crabs: Future Research Directions

Gopal

Sivanesan

Muthu

et al. 2022

Foods

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While plastics are already notorious for their accumulation in the environment, which poses environmental challenges, invisible microplastics (MPS) are an even greater challenge. This review focuses on consolidating the reports available on MP accumulation in edible marine and freshwater fishes, shrimps, and crabs. The reality as to whether MPs in these edible aquatic organisms are really a cause of high concern is questioned and discussed. While the entrails of aquatic organisms are reported to contain high levels of MPs, because these products are consumed after the removal of the entrails and gut area in the majority of cases, the MP threat is questionable. The existence of MPs in these aquatic sources is validated but their potency in harming humans, aquatic organisms, and other interlinked species is unassessed. To overcome the difficulty in tracing the movement of MPs in a bigger ecosystem, this review proposes laboratory-based pilot studies mimicking real-world conditions, which will help us to understand the kinetics of MPs in the food chain. The effects of MPs on human welfare and health are yet to be assessed, and this is another gap that needs attention.

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Metabolomic disorders unveil hepatotoxicity of environmental microplastics in wild fish Serranus scriba (Linnaeus 1758)

Cited by 33 publications

References 84 publications

Differential Cell Metabolic Pathways in Gills and Liver of Fish (White Seabream Diplodus sargus) Coping with Dietary Methylmercury Exposure

Differential Cell Metabolic Pathways in Gills and Liver of Fish (White Seabream Diplodus sargus) Coping with Dietary Methylmercury Exposure

Ameliorative Hematological and Histomorphological Effects of Dietary Trigonella foenum-graecum Seeds in Common Carp (Cyprinus carpio) Exposed to Copper Oxide Nanoparticles

Overviewing the Ground Reality of Microplastic Effects on Seafoods, Including Fish, Shrimps and Crabs: Future Research Directions

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