2013
DOI: 10.1167/iovs.12-11451
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Metabolomic Changes in the Rat Retina After Optic Nerve Crush

Abstract: Citation: Agudo-Barriuso M, Lahoz A, Nadal-Nicolás FM, et al. Metabolomic changes in the rat retina after optic nerve crush. Invest Ophthalmol Vis Sci. 2013;54:4249-4259. DOI:10.1167/iovs.12-11451 PURPOSE. To identify metabolic pathways and metabolites affected by optic nerve crush that can act as predictors of the disease or therapeutic targets. METHODS.The left optic nerve of adult rats was intraorbitally crushed and retinas were dissected 24 hours or 14 days after the lesion (n ¼ 10 per group). Metaboli… Show more

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Cited by 41 publications
(30 citation statements)
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“…The present studies rely on immunostaining several proteins to identify different retinal neurons, and thus caution should be taken when interpreting the results because retinal injury may modify the expression of many genes (Agudo et al, 2008Agudo-Barriuso et al, 2013b;Chidlow et al, 2005); this may result in lower expression of the proteins and their epitopes. Nevertheless, both melanopsin and Brn3a have been shown to be expressed long after ON injury (Galindo-Romero et al, 2013b;Sánchez-Migallón et al, 2011).…”
Section: Discussionmentioning
confidence: 99%
“…The present studies rely on immunostaining several proteins to identify different retinal neurons, and thus caution should be taken when interpreting the results because retinal injury may modify the expression of many genes (Agudo et al, 2008Agudo-Barriuso et al, 2013b;Chidlow et al, 2005); this may result in lower expression of the proteins and their epitopes. Nevertheless, both melanopsin and Brn3a have been shown to be expressed long after ON injury (Galindo-Romero et al, 2013b;Sánchez-Migallón et al, 2011).…”
Section: Discussionmentioning
confidence: 99%
“…27,28,42 Optic nerve crush is a model that has been widely used to test neuroprotective therapies 36,37,41,[43][44][45][46][47] and to investigate the molecular events underlying central nervous system neuronal death upon axonal trauma. 25,48,49 Optic nerve crush is as well often used as a simplified model of glaucomatous injury. To the best of our knowledge there are no previous studies investigating the effects of topical administration of a NSAID on the retinal inflammation after complete optic nerve axotomy.…”
mentioning
confidence: 99%
“…The advance of genetic therapy focused mainly on earlier detection and treatment of complex pathologies from congenital or acquired primary human source has been motivating an increasing number of ophthalmologists to develop critical analyses about the functional role of basic science applied to the improvement of patients' health at the bedside. Analyses of genomic, transcript omic, proteomic, and metabolomic technologies have demonstrated an integrative function in the investigation and treatment of pathophysiological processes with a higher degree of complexity, related to eye diseases at levels of DNA, RNA, protein and cell/tissue, respectively [32][33][34][35][36][37][38]. Furthermore, the use of protein and antibody arrays [39] and the next-generation DNA sequencing technology [40,41] supports the earlier detection by screening of proteomic, transcript omic and genomic profiles, respectively, in eye diseases and ocular syndromes which are well-defined in clinical practice, but not fully-investigated for gene therapy approaches and treatment outcome.…”
Section: Reverse Translational Researchmentioning
confidence: 99%