Metabolomic analysis of the consequences of cadmium exposure in Silene cucubalus cell cultures via 1H NMR spectroscopy and chemometrics

Bailey, Nigel J.; Oven, Matjaž; Holmes, Elaine; Nicholson, Jeremy K.; Zenk, Meinhart H.

doi:10.1016/s0031-9422(02)00719-7

Cited by 116 publications

(68 citation statements)

References 25 publications

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“…The components (metabolites) of the metabolome, as the end products of metabolisms represent the functional responses of a cell. The quantitative measurements of large numbers of endogenous metabolites thus provide a broad view of the biochemical status of an organism that can be used to monitor and assess the metabolic responses induced by exogenous factors, such as environmental stressors (Fiehn 2002;Pedras and Zheng 2010;Sun et al 2010;Bailey et al 2003;Park et al 2009;Manetti et al 2004). This technique has been widely applied in environmental sciences with the term environmental metabolomics (Bundy et al 2004(Bundy et al , 2009.…”

Section: Introductionmentioning

confidence: 99%

“…Environmental metabolomics on the plant usually performs studies on the plant-environment interactions and for assessing plant function and health at the molecular level, and focuses on the metabolic responses to the environmental changes (drought, salinity, etc. ), abiotic stresses (contaminates), and transgenic events (Aliferis et al 2009;Bundy et al 2009;Eisenreich and Bacher 2007;Sun et al 2010;Bailey et al 2003;Manetti et al 2004;Park et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics

et al. 2011

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Cadmium is a non-essential element to living organisms and has become the severe contaminant in both seawater and sediment in the intertidal zones of the Bohai Sea. The halophyte, Suaeda salsa is the pioneer plant in the intertidal zones of Bohai Sea and has been widely applied in environmental sciences. In this study, the dose-and time-dependent effects induced by environmentally relevant concentrations (2, 10 and 50 lg l -1 ) of cadmium were characterized in S. salsa using NMR-based metabolomics. The levels of amino acids (valine, leucine, glutamate, tyrosine, etc.), carbohydrates (glucose, sucrose and fructose), intermediates of tricarboxylic acid cycle (succinate, citrate, etc.) and osmolyte (betaine) were altered in the S. salsa samples after cadmium exposures. These metabolic biomarkers indicated the elevated protein degradation and disturbances in the osmotic regulation and energy metabolism caused by cadmium in S. salsa. Overall, our results demonstrated the applicability of NMR-based metabolomics for the detection of metabolic biomarkers that could be used for the interpretation of toxicological effects induced by contaminants in the pioneer plant S. salsa in the intertidal zones. In addition, the metabolic biomarkers could be potentially useful for the bio-monitoring of contaminants in the intertidal zones.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics

et al. 2011

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“…Therefore, environmental metabolomics has been found to be applicable in the environmental sciences on the basis of metabolic responses to environmental factors. The quantitative measurements of large numbers of endogenous metabolites thus can provide a broad view of the biochemical status of an organism that can be used to assess the metabolic responses induced by exogenous factors, such as environmental stressors (Fiehn 2002;Pedras and Zheng 2010;Sun et al 2010;Bailey et al 2003). Practically, metabolomics can measure the metabolic changes with modern analytical techniques such as nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (Lindon et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Liu

You

et al. 2011

Plant Mol Biol Rep

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Suaeda salsa is a native halophyte in saline soils. Salinity is the most important environmental constraint for plant productivity in the Yellow River Delta. In this work, we investigated the salt-induced effects in root of S. salsa exposed to two environmentally relevant salinities for 1 week and 1 month using nuclear magnetic resonance-based metabolomics. Our results indicated that salt stress inhibited the growth of S. salsa and induced significant metabolic responses including decreased amino acids, lactate, 4-aminobutyrate, malate, choline, phosphocholine, and increased betaine, sucrose, and allantoin in root tissues of S. salsa. In addition, salinity exposures upregulated the activities of superoxide dismutase, glutathione S-transferases, peroxidase, catalase, and glutathione peroxidase in the aboveground part of seedlings of S. salsa after exposures. Overall, these results demonstrated the osmotic and oxidative stresses, disturbances in protein biosynthesis/degradation, and energy metabolism in S. salsa exposed to salinities.

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“…Whereas genomics and proteomics can provide insights into the potential of a biological system to interact with external perturbations, it is the resulting changes in the metabolic profile of the system that are potentially more useful for the understanding of the biochemical reaction to stress (Bailey et al, 2003).…”

mentioning

confidence: 99%

Metabolic Discrimination ofCatharanthus roseusLeaves Infected by Phytoplasma Using 1H-NMR Spectroscopy and Multivariate Data Analysis

et al. 2004

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A comprehensive metabolomic profiling of Catharanthus roseus L. G. Don infected by 10 types of phytoplasmas was carried out using one-dimensional and two-dimensional NMR spectroscopy followed by principal component analysis (PCA), an unsupervised clustering method requiring no knowledge of the data set and used to reduce the dimensionality of multivariate data while preserving most of the variance within it. With a combination of these techniques, we were able to identify those metabolites that were present in different levels in phytoplasma-infected C. roseus leaves than in healthy ones. The infection by phytoplasma in C. roseus leaves causes an increase of metabolites related to the biosynthetic pathways of phenylpropanoids or terpenoid indole alkaloids: chlorogenic acid, loganic acid, secologanin, and vindoline. Furthermore, higher abundance of Glc, Glu, polyphenols, succinic acid, and Suc were detected in the phytoplasma-infected leaves. The PCA of the 1 H-NMR signals of healthy and phytoplasma-infected C. roseus leaves shows that these metabolites are major discriminating factors to characterize the phytoplasma-infected C. roseus leaves from healthy ones. Based on the NMR and PCA analysis, it might be suggested that the biosynthetic pathway of terpenoid indole alkaloids, together with that of phenylpropanoids, is stimulated by the infection of phytoplasma.

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Metabolomic analysis of the consequences of cadmium exposure in Silene cucubalus cell cultures via 1H NMR spectroscopy and chemometrics

Cited by 116 publications

References 25 publications

Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics

Metabolic profiling of cadmium-induced effects in one pioneer intertidal halophyte Suaeda salsa by NMR-based metabolomics

Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Metabolic Discrimination ofCatharanthus roseusLeaves Infected by Phytoplasma Using 1H-NMR Spectroscopy and Multivariate Data Analysis

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