Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Wu, Huifeng; Liu, Xiaoli; You, Liu; Zhang, Linbao; Yu, Junbao; Zhou, Di; Zhao, Jianmin; Feng, Jianghua

doi:10.1007/s11105-011-0368-4

Cited by 14 publications

(5 citation statements)

References 51 publications

(53 reference statements)

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“…7). This result is consistent with the metabolic profiles of the S. salsa response to salinity (Wu et al 2011). The down-regulation in these metabolites indicated that salinity induced disturbances in the TCA cycle.…”

Section: Discussionsupporting

confidence: 89%

“…Polar metabolites were extracted from the leaves of S. corniculata using the solvent system of methanol/water (1/1), as described previously (Wu et al 2011). Six replicates were used for each treatment.…”

Section: Metabolite Extractionmentioning

confidence: 99%

“…Metabolites were assigned and quantified following the tabulated chemical shifts (Wu et al 2011Ji et al 2013) and using the software Chenomx (Evaluation Version, Chenomx Inc., Canada). We used principal components analysis (PCA) in this work for the separation of control and different salt exposed groups.…”

Section: Data Processing and Statistical Analysismentioning

confidence: 99%

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Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

et al. 2016

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Background and aims Soil alkalinity and salinity are two of the most common environmental stress factors that impact plant growth and productivity. S. corniculata is native to the saline-alkali soil of Northeast China which shows a higher alkali tolerance than other suaeda plants. It will be very important to identify the effect of salt and alkali stress on S. corniculata through proteomics and metabolomics analysis to improve understanding of the resistance of plants. Methods S. corniculata seedlings were exposed to salt and alkali stress for 72 h, respectively. Metabolic changes were quantified by conducting MS-based proteomics and NMR-based metabolomics analysis.Results The response of S. corniculata to salt stress was distinct from that of plants subjected to alkali stress at both physiological and molecular levels. The integrated-omic studies identified 22 and 19 differentially expressed proteins and metabolites mainly involved in carbohydrate metabolism and amino acid metabolism. Conclusions On account of the up-regulation of energy metabolism and higher accumulation of organic osmolytes, S. corniculata shows high pH resistance when it suffers alkali stress. These findings provide insights into the different regulatory mechanisms in halophyte S. corniculata response to salt and alkali stresses.

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Section: Discussionsupporting

confidence: 89%

Section: Metabolite Extractionmentioning

confidence: 99%

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Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

et al. 2016

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“…Increased levels of proline were previously documented in other crops too, i.e., rice [ 58 ] and maize [ 59 ], etc., which aligns with the findings of the present study. Wu et al [ 60 ] demonstrated that the contents of proline, allantoin, and sucrose were increased in Suaeda salsa , adjusting osmoregulation. Selaginella lepidophylla accumulated an abundant amount of allantoin in a state of dehydration, indicating a protective role in survival and recovery from stress [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Exogenous Allantoin Confers Rapeseed (Brassica campestris) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology

et al. 2023

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Allantoin is an emerging plant metabolite, but its role in conferring drought-induced oxidative stress is still elusive. Therefore, an experiment was devised to explore the role of allantoin (0.5 and 1.0 mM; foliar spray) in rapeseed (Brassica campestris cv. BARI Sarisha-17) under drought. Seedlings at fifteen days of age were subjected to drought, maintaining soil moisture levels at 50% and 25% field capacities, while well-irrigated plants served as the control group. Drought-stressed plants exhibited increased levels of lipid peroxidation and hydrogen peroxide, electrolyte leakage, and impaired glyoxalase systems. Thus, the growth, biomass, and yield attributes of rapeseed were significantly impaired under drought. However, the allantoin-supplemented plants showed a notable increase in their contents of ascorbate and glutathione and decreased dehydroascorbate and glutathione disulfide contents under drought. Moreover, the activity of antioxidant enzymes such as ascorbate peroxidase, dehydroascorbate reductase, glutathione reductase, glutathione peroxidase, and catalase were accelerated with the allantoin spray and the glyoxalase system was also enhanced under drought. Moreover, the improvement in water balance with reduction in proline and potassium ion contents was also observed when allantoin was applied to the plants. Overall, the beneficial effects of allantoin supplementation resulted in the improved plant growth, biomass, and yield of rapeseed under drought conditions. These findings suggest that allantoin acts as an efficient metabolite in mitigating the oxidative stress caused by reactive oxygen species by enhancing antioxidant defense mechanisms and the glyoxalase system.

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“…The principal component score plots visualize oyster samples, and each dot in the principal component analysis figure stands for an individual oyster gill sample. The highest amount of correlated variation along the principal component 1 axis, together with subsequent principle components containing smaller amounts of variance, are calculated, based on the algorithm of principal component analysis .…”

Section: Methodsmentioning

confidence: 99%

Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR‐based metabolomics

Cao

Wang

2017

Enviro Toxic and Chemistry

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Traditional metal toxicity tests on organisms have mainly focused on continuous exposure at a fixed concentration. However, organisms are more likely exposed to pollutants intermittently in estuarine environments that are significantly impacted by anthropogenic activity. The present study examined whether different copper (Cu) exposure regimes at an equivalent dose can induce different metabolomics effects on the oysters. An estuarine oyster Crassostrea hongkongensis was exposed to Cu continuously or intermittently at an equal dose (time × concentration) for 6 wk. Continuous exposure regimes included 2 doses of 3.3 μg/L for 24 h and 20 μg/L for 24 h, with corresponding equal doses of 2 intermittent exposure regimes of 20 μg/L for 4 h and 120 μg/L for 4 h, respectively. Time-course measurements suggested that Cu bioaccumulation was comparable at equal low doses between the continuous regime (3.3 μg/L for 24 h) and the intermittent regime (20 μg/L for 4 h), but there was considerable difference for the high dose under different regimes. Nuclear magnetic resonance (NMR)-based metabolomics suggested that continuous and intermittent Cu exposures led to similar metabolite variation pattern in gills at an equal high dose, including decreased amino acids (e.g., aspartate, glycine, isoleucine, leucine, lysine, phenylalanine, threonine, and valine), lower energy-related compounds (e.g., adenosine triphosphate/adenosine diphosphate, acetate, citrate, and glycogen), and altered osmolytes (e.g., homarine and taurine). These biomarkers indicated disturbance of osmotic regulation and energy metabolism induced by Cu exposure regardless of regime. In addition, the 4-h intermittent Cu exposure resulted in slightly fewer adverse effects compared with the corresponding equal-dose continuous exposure. Oysters appeared to recover during the intervals of Cu exposure. The results indicated that metabolomic effects induced by Cu were more dose dependent than the Cu exposure regime. Environ Toxicol Chem 2017;36:2428-2435. © 2017 SETAC.

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Salinity-Induced Effects in the Halophyte Suaeda salsa Using NMR-based Metabolomics

Cited by 14 publications

References 51 publications

Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

Integrated proteomics and metabolomics for dissecting the mechanism of global responses to salt and alkali stress in Suaeda corniculata

Exogenous Allantoin Confers Rapeseed (Brassica campestris) Tolerance to Simulated Drought by Improving Antioxidant Metabolism and Physiology

Chronic effects of copper in oysters Crassostrea hongkongensis under different exposure regimes as shown by NMR‐based metabolomics

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