Metabolome Comparison of Bioactive and Inactive <i>Rhododendron</i> Extracts and Identification of an Antibacterial Cannabinoid(s) from <i>Rhododendron collettianum</i>

Said, Inamullah Hakeem; Rezk, Ahmed; Hussain, Ishtiaq; Grimbs, Anne; Shrestha, Abhinandan; Schepker, Hartwig; Brix, Klaudia; Ullrich, Matthias S.; Kuhnert, Nikolai

doi:10.1002/pca.2694

Cited by 22 publications

(7 citation statements)

References 40 publications

(44 reference statements)

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“…was compared to a series of inactive extracts. Three metabolites were found to distinguish R. collettianum from other species indicating the ability to suggest potential bioactive substances (Hakeem Said et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Recently, there has been growing interests to find antimicrobial compounds from medicinal plant extracts as an alternative approach to discover new antimicrobial compounds (Ríos and Recio, 2005;Radji et al, 2013). Rhododendron species have been proved to possess medicinal and health promotion properties, including the ability to inhibit the growth of some types of pathogenic bacteria (Innocenti et al, 2010;Silici et al, 2010;Popescu and Kopp, 2013;Rezk et al, 2015;Wang et al, 2015;Li et al, 2016;Hakeem Said et al, 2017;Shrestha et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…These species are known to be a good source of polyphenolic plant secondary plant metabolites . The plant extracts belonging to the genus Rhododendron and isolated compounds demonstrated diverse biological activities including anti-inflammatory, analgesic, anti-microbial, antidiabetic, insecticidal and cytotoxic activity (Popescu and Kopp, 2013), as well as antibacterial activity (Innocenti et al, 2010;Silici et al, 2010;Popescu and Kopp, 2013;Rezk et al, 2015;Wang et al, 2015;Li et al, 2016;Hakeem Said et al, 2017;Shrestha et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Activities of Ethanolic Extract Obtained from Rhododendron Myrtifolium Schott & Kotschy Leaves Against Clinically Isolated Bacterial Strains

Honcharenko¹,

Tkachenko²,

Prokopiv³

et al. 2019

Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019

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The plants belonging to the genus Rhododendron L. and isolated compounds demonstrate diverse biological activities including anti-inflammatory, analgesic, anti-microbial, anti-diabetic, insecticidal and cytotoxic activity, as well as antibacterial activity. Our study was designed the effects of Rhododendron myrtifolium Schott & Kotschy leaf extract against bacterial strains were monitored in vitro by the disk diffusion method. The aim of this study was to assess possible antibacterial effects of an ethanolic extract derived from Rhododendron myrtifolium leaves against Citrobacter freundii, Enterobacter cloacae, Klebsiella pneumoniae, and Escherichia coli strain locally isolated from human biological fluids. Thus, the study contributes to on-going investigations on the bioactivity potential of plant species such as the Rhododendron. Leaves of Rhododendron myrtifolium were harvested on the side of the road between the Menchul valley and Rogneska valley (Kvasy village, Rakhiv district, Zakarpattia region, Ukraine). The results revealed that extract exerts antibacterial activity against Citrobacter freundii. However, the Enterobacter cloacae, Klebsiella pneumoniae, and Escherichia coli were resistant to R. myrtifolium leaf extract. Maximum in vitro inhibition was scored against Citrobacter freundii, followed by Escherichia coli, Klebsiella pneumoniae, and Enterobacter cloacae, which presented inhibition zones of (12.1 ±0.9) mm, (9.1 ±0.5) mm, (7.5 ±0.6) mm, and (7.2 ±0.5) mm, respectively. In the case of the positive controls, 96% ethanol possesses a mild antibacterial effect, which presented inhibition zones of (6.5 ±0.7) mm. The results from the screening study performed by the disc diffusion method revealed that R. myrtifolium possesses a mild antibacterial activity against C. freundii. However, further investigation is needed to determine the bioavailability of the active compounds and to determine the dose and toxicity before it can be used as therapeutic agents.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibacterial Activities of Ethanolic Extract Obtained from Rhododendron Myrtifolium Schott & Kotschy Leaves Against Clinically Isolated Bacterial Strains

Honcharenko¹,

Tkachenko²,

Prokopiv³

et al. 2019

Agrobiodiversity for Improving of Nutrition, Health and Life Quality 2019

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“…Bioactive diterpenoids, triterpenoids and polyphenolics have been previously elucidated from this genus [ 41 , 48 ]. The presence of the specific mono-, di-, or sesquiterpenoids in crude extract makes rhododendron plants a good candidate for antibacterial agents [ 49 ]. It is used as an expectorant and for chronic bronchitis in traditional medicine together with its anti-inflammatory properties for treating rheumatoid arthritis [ 48 , 50 ].…”

Section: Non-cannabis Sourcesmentioning

confidence: 99%

Mass Spectrometry-Based Metabolomics of Phytocannabinoids from Non-Cannabis Plant Origins

et al. 2022

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Phytocannabinoids are isoprenylated resorcinyl polyketides produced mostly in glandular trichomes of Cannabis sativa L. These discoveries led to the identification of cannabinoid receptors, which modulate psychotropic and pharmacological reactions and are found primarily in the human central nervous system. As a result of the biogenetic process, aliphatic ketide phytocannabinoids are exclusively found in the cannabis species and have a limited natural distribution, whereas phenethyl-type phytocannabinoids are present in higher plants, liverworts, and fungi. The development of cannabinomics has uncovered evidence of new sources containing various phytocannabinoid derivatives. Phytocannabinoids have been isolated as artifacts from their carboxylated forms (pre-cannabinoids or acidic cannabinoids) from plant sources. In this review, the overview of the phytocannabinoid biosynthesis is presented. Different non-cannabis plant sources are described either from those belonging to the angiosperm species and bryophytes, together with their metabolomic structures. Lastly, we discuss the legal framework for the ingestion of these biological materials which currently receive the attention as a legal high.

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“…Meroterpenoids from Rhododendron plants have attracted attention from the natural product community due to their diverse structures and intriguing bioactivities. Our previous investigations on this genus resulted in the discovery of an array of meroterpenoids with anti‐inflammatory effects, antiviral activity, and protein tyrosine phosphatase 1B inhibition 14–18 . In this study, rhodomeroterpene (RMT), a new meroterpenoid isolated from the Rhododendron genus, was investigated for its anti‐inflammatory activity and protective effect against AKI.…”

Section: Introductionmentioning

confidence: 99%

Rhodomeroterpene alleviates macrophage infiltration and the inflammatory response in renal tissue to improve acute kidney injury

Shi

Zhu

et al. 2021

The FASEB Journal

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Inflammation is broadly recognized as an important factor in the pathogenesis of acute kidney injury (AKI), but pharmacological approaches to alleviate inflammation in AKI have not been proved successful in clinical trials. Macrophage infiltration into renal tissue promotes inflammatory responses that contribute to the pathogenesis of AKI. Suppression of renal tissue inflammatory responses is postulated to improve renal injury of patients and animals. Rhodomeroterpene (RMT) is a novel meroterpenoid isolated from the Rhododendron genus that was shown to exert anti-inflammatory action in vivo or in vitro in this study. We investigated the treatment effects of RMT on LPS-induced sepsis and two different AKI models. The results showed that pretreatment with RMT (30 mg kg −1 d −1 , ip, for 3 days) significantly inhibited acute inflammatory responses in LPSinduced septic mice. In both renal ischemia-reperfusion injury (I/R) and sepsisinduced AKI models, RMT (30 mg kg −1 d −1 , ip, for 3 days) ameliorated renal function and injury and alleviated inflammation by reducing the infiltration of immune cells, including macrophages and neutrophils. Furthermore, our study demonstrated that RMT inhibits inflammatory responses in macrophages. The anti-inflammatory effects of RMT may be due to the inactivation of the IKK/ NF-κB and PI3K/PDK1/Akt inflammatory signaling pathways in macrophages.Collectively, our findings indicate that RMT ameliorates renal injury and alleviates the renal inflammatory state in different AKI models, suggesting that RMT may be a potential agent for the treatment of AKI.

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Metabolome Comparison of Bioactive and Inactive Rhododendron Extracts and Identification of an Antibacterial Cannabinoid(s) from Rhododendron collettianum

Abstract: It was shown that bioinformatics tools facilitate early stage identification of a biologically active compound(s) using LC-MS data, which reduce complexity and number of separation steps in bioactive screening. Copyright © 2017 John Wiley & Sons, Ltd.

Cited by 22 publications

References 40 publications

Antibacterial Activities of Ethanolic Extract Obtained from Rhododendron Myrtifolium Schott & Kotschy Leaves Against Clinically Isolated Bacterial Strains

Antibacterial Activities of Ethanolic Extract Obtained from Rhododendron Myrtifolium Schott & Kotschy Leaves Against Clinically Isolated Bacterial Strains

Mass Spectrometry-Based Metabolomics of Phytocannabinoids from Non-Cannabis Plant Origins

Rhodomeroterpene alleviates macrophage infiltration and the inflammatory response in renal tissue to improve acute kidney injury

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