Metabolites of Ammopiptanthus mongolicus induced by Orgyia ericae attack and mechanical wounding

Sun, Jingru; Yang, Haijun; Ting, Zhang; Cao, Chuanjian; Zong, Shixiang; Luo, Youqing; Shen, Yingbai

doi:10.1016/j.plaphy.2013.04.026

Cited by 6 publications

(11 citation statements)

References 35 publications

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“…And there was no mark of TIAs in stems under RT group. It indicates that the responses of RT group to wounding take place at the site of injure, that is local responses, where the metabolites were directly used for mechanical defense (Sun et al 2013). By contrast, the major site of action of these significantly different metabolites was stems in LT group.…”

Section: Discussionmentioning

confidence: 92%

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The specific responses to mechanical wound in leaves and roots of Catharanthus roseus seedlings by metabolomics

Chen

Guo

et al. 2018

Journal of Plant Interactions

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The mechanical wound is one of the unavoidable threats to survival of plants. More researchers focus on the effect of mechanical wound to the over-ground tissues. And the effects of wound to roots were frequently ignored, although it is an important organ for plant growth. In our studies, the metabolomics study was performed to reveal the mechanical wound effects in Catharanthus roseus on roots and leaves by combining gas chromatography-mass spectrometer (GC-MS), liquid chromatograph-mass spectrometer (LC-MS) and statistical analyses. The metabolic response of TIAs and PCs in plants to wound was most active at 0.5 h after treatment via Q value analysis. At this time point, then significantly responsive primary metabolites and specific secondary compounds (TIAs and PCs) were screened by PLS-DA score plot. In this case, the treatments of CK, LT (wound to leaves) and RT (wound to roots) were clearly distinguished. The targeted compounds include 8 sugars, 4 TIAs and 12 PCs and they displayed specific responses to CK, LT and RT treatments. Under RT group, plants invest more resources on the local responses using TIAs and the color reactions to regulate wound close using PCs. Whereas, LT group might lay emphasis on systemic responses via TIAs induced by SA (salicylic acid) and gallic acid. Our studies provide some basic data for further investigations of the defensive mechanism on roots treated by mechanical wound.

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Section: Discussionmentioning

confidence: 92%

“…By contrast, the major site of action of these significantly different metabolites was stems in LT group. Systemic response adopted by LT group and need time to regulations (Sun et al 2013). Therefore, different responses style was adopted between RT group and LT group in terms of TIAs accumulation.…”

Section: Discussionmentioning

confidence: 99%

The specific responses to mechanical wound in leaves and roots of Catharanthus roseus seedlings by metabolomics

Chen

Guo

et al. 2018

Journal of Plant Interactions

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“…Metabolites in roots were identified according to our previous results [19] and literature data [18], [24], [25], 2D NMR spectra or pure compounds and the 1 H chemical shifts and coupling constants of identified signals were listed in table 1. Metabolites identified from leaves and roots included fatty acids, organic acids, amino acids, sugars, alcohols, aromatic compounds, alkaloids and isoflavones.…”

Section: Resultsmentioning

confidence: 99%

“…Leaves and roots were extracted according to the method used by Kim et al [18] with some modifications as in our previous study [19]. Plant materials were grinded with pestle and mortar under liquid nitrogen into fine powder, and then were freeze-dried for 24 h. After weighting dried samples, solvent containing 0.75 ml CD 3 OD and 0.75 ml KH 2 PO 4 buffer in D 2 O (pH 6.0) with 0.05% (wt/wt) TSP were added into each centrifuge tube containing dried powder of fifty milligram leaves or forty milligram roots.…”

Section: Methodsmentioning

confidence: 99%

Similar Metabolic Changes Induced by HIPVs Exposure as Herbivore in Ammopiptanthus mongolicus

Sun

Zhang

Cao³

et al. 2014

PLoS ONE

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Herbivore-induced plant volatiles (HIPVs) are important compounds to prim neighboring undamaged plants; however, the mechanism for this priming process remains unclear. To reveal metabolic changes in plants exposed to HIPVs, metabolism of leaves and roots of Ammopiptanthus mongolicus seedlings exposed to HIPVs released from conspecific plants infested with larvae of Orgyia ericae were analyzed together with control and infested seedlings using nuclear magnetic resonance (NMR)-based metabolic technology and multi variate data analysis. Results presented showed that HIPVs exposure led to similar but specific metabolic changes compared with those induced by infestation in both leaves and roots. Furthermore, both HIPVs exposure and herbivore attack resulted in metabolic changes involving a series of primary and secondary metabolites in both leaves and roots. Taken together, these results suggested that priming of yet-damaged plants may be achieved by reconfiguring metabolic pathways in leaves and roots to make similar concentrations for all metabolites as those in seedlings infested. Therefore, we propose that improved readiness of defense induction of primed plants toward subsequent herbivore attack may be based on the similar metabolic profiling induced by HIPVs exposure as those caused by herbivore.

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“…76 This method can also distinguish compounds with identical masses, including those with different isotopomer distributions, 77 and compounds that exhibit similar elution behavior in conventional chromatography. For instance, the isomers 3-Oand 5-O-caffeoylquinic acid, which are challenging to Saccharides and glycosylic portion stachyose, 34,35 rhamnose in rutin, 36 pinitol, 37 rhamnose in flavonoid, 38 raffinose, 36 gentisic acid glucoside, 36 neochlorogenic acid (3-O-caffeoylquinic acid), 33,35,38,39,43,63,64 4-O-caffeoylquinic acid, 38,63 chlorogenic acid (5-O-caffeoylquinic acid), 33,35,36,38,41,43,56,57,63,65 trans-caffeoyl derivatives, 50 trans-feruloyl derivatives, 50 trans-caftaric acid, 50 trans-fertaric acid, 50 caffeoyl esters of polyhydroxy compounds, 54 p-coumaric acid, 58 5-O-feruloyl quinic acid, 35,43 trans-cinnamic acid 50 Furano lactones limonin, 59,66 limonin glucoside 59,66,67 Glucosinolates neoglucobrassicin, 28 progoitrin, 28,30 gluconapin, 48 sinigrin 51 Alkaloids synephrine, 59,66,67 nicotine, 38 vindoline, 57 …”

Section: Potentials Of 1 H Nmr In Plant Researchmentioning

confidence: 99%

Twenty Years of ¹H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress

Mascellani Bergo,

Leiss,

Havlik

2024

J. Agric. Food Chem.

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Metabolomics has become an important tool in elucidating the complex relationship between a plant genotype and phenotype. For over 20 years, nuclear magnetic resonance (NMR) spectroscopy has been known for its robustness, quantitative capabilities, simplicity, and cost-efficiency. 1 H NMR is the method of choice for analyzing a broad range of relatively abundant metabolites, which can be used for both capturing the plant chemical profile at one point in time and understanding the pathways that underpin plant defense. This systematic Review explores how 1 H NMR-based plant metabolomics has contributed to understanding the role of various compounds in plant responses to biotic stress, focusing on both primary and secondary metabolites. It clarifies the challenges and advantages of using 1 H NMR in plant metabolomics, interprets common trends observed, and suggests guidelines for method development and establishing standard procedures.

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Metabolites of Ammopiptanthus mongolicus induced by Orgyia ericae attack and mechanical wounding

Cited by 6 publications

References 35 publications

The specific responses to mechanical wound in leaves and roots of Catharanthus roseus seedlings by metabolomics

The specific responses to mechanical wound in leaves and roots of Catharanthus roseus seedlings by metabolomics

Similar Metabolic Changes Induced by HIPVs Exposure as Herbivore in Ammopiptanthus mongolicus

Twenty Years of ¹H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress

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Metabolites of Ammopiptanthus mongolicus induced by Orgyia ericae attack and mechanical wounding

Cited by 6 publications

References 35 publications

The specific responses to mechanical wound in leaves and roots of Catharanthus roseus seedlings by metabolomics

The specific responses to mechanical wound in leaves and roots of Catharanthus roseus seedlings by metabolomics

Similar Metabolic Changes Induced by HIPVs Exposure as Herbivore in Ammopiptanthus mongolicus

Twenty Years of 1H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress

Contact Info

Product

Resources

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Twenty Years of ¹H NMR Plant Metabolomics: A Way Forward toward Assessment of Plant Metabolites for Constitutive and Inducible Defenses to Biotic Stress