Metabolites from the Dark Septate Endophyte <i>Drechslera</i> sp. Evaluation by <scp>LC</scp>/<scp>MS</scp> and Principal Component Analysis of Culture Extracts with Histone Deacetylase Inhibitors

Siless, Gastón Ezequiel; Gallardo, Gabriela L.; Rodríguez, María Alejandra; Rincón, Yuliet A; Godeas, Alicia Margarita; Cabrera, Gabriela M.

doi:10.1002/cbdv.201800133

Cited by 22 publications

(10 citation statements)

References 55 publications

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“…Some of the core and most abundant taxa, such as Darksidea sp. , Periconia macrospinosa, Slopeiomyces cylindrosporus and Drechslera sp., were previously reported as DSE in other grasses (Hornby et al, 1977; Knapp et al, 2012, 2015; Siless et al, 2018). In addition, Helotiales sp.…”

Section: Discussionmentioning

confidence: 55%

A Survey of Culturable Fungal Endophytes From Festuca rubra subsp. pruinosa, a Grass From Marine Cliffs, Reveals a Core Microbiome

et al. 2019

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Festuca rubra subsp. pruinosa is a perennial grass that inhabits sea cliffs of the Atlantic coasts of Europe. In this unhospitable environment plants grow in rock crevices and are exposed to abiotic stress factors such as low nutrient availability, wind, and salinity. Festuca rubra subsp. pruinosa is a host of the fungal endophyte Epichloë festucae, which colonizes aerial organs, but its root mycobiota is unknown. The culturable endophytic mycobiota of FRP roots was surveyed in a set of 105 plants sampled at five populations in marine cliffs from the northern coast of Spain. In total, 135 different fungal taxa were identified, 17 of them occurred in more than 10% of plants and in two or more populations. Seven taxa belonging to Fusarium, Diaporthe, Helotiales, Drechslera, Slopeiomyces, and Penicillium appeared to be constituents of the core microbiome of Festuca rubra subsp. pruinosa roots because they occurred in more than 20% of the plants analyzed, and at three or more populations. Most fungal strains analyzed (71.8%) were halotolerant. The presence of Epichloë festucae in aboveground tissue was detected in 65.7% of the plants, but its presence did not seem to significantly affect the structure of the core or other root microbiota, when compared to that of plants free of this endophyte. When plants of the grass Lolium perenne were inoculated with fungal strains obtained from Festuca rubra subsp. pruinosa roots, a Diaporthe strain significantly promoted leaf biomass production under normal and saline (200 mM NaCl) watering regimes. These results suggest that the core mycobiome of Festuca rubra subsp. pruinosa could have a role in host plant adaptation, and might be useful for the improvement of agricultural grasses.

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Section: Discussionmentioning

confidence: 55%

A Survey of Culturable Fungal Endophytes From Festuca rubra subsp. pruinosa, a Grass From Marine Cliffs, Reveals a Core Microbiome

et al. 2019

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“…(Du et al, 2014), Drechslera sp. (Siless et al, 2018), Microascus sp. (Vervoort et al, 2011), and M. yucatanensis (Qadri et al, 2017).…”

Section: Chemical Inhibition Of Epigenetic Machinerymentioning

confidence: 99%

“…OHA is an analog of SAHA which has induced production in Drechslera sp. (Siless et al, 2018). SBHA has also been widely used in B. bassiana (Yakasai et al, 2011), Cordyceps annullata (Asai et al, 2012b), C. cladosporoides (Williams et al, 2008), C. indigotica (Asai et al, 2012e), I. tenuipes (Asai et al, 2012a), and Torrubiella luterorostrata (Asai et al, 2011).…”

Section: Chemical Inhibition Of Epigenetic Machinerymentioning

confidence: 99%

On top of biosynthetic gene clusters: How epigenetic machinery influences secondary metabolism in fungi

Pfannenstiel

Keller

2019

Biotechnology Advances

119

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Fungi produce an abundance of bioactive secondary metabolites which can be utilized as antibiotics and pharmaceutical drugs. The genes encoding secondary metabolites are contiguously arranged in biosynthetic gene clusters (BGCs), which supports co-regulation of all genes required for any one metabolite. However, an ongoing challenge to harvest this fungal wealth is the finding that many of the BGCs are ‘silent’ in laboratory settings and lie in heterochromatic regions of the genome. Successful approaches allowing access to these regions - in essence converting the heterochromatin covering BGCs to euchromatin - include use of epigenetic stimulants and genetic manipulation of histone modifying proteins. This review provides a comprehensive look at the chromatin remodeling proteins which have been shown to regulate secondary metabolism, the use of chemical inhibitors used to induce BGCs, and provides future perspectives on expansion of epigenetic tools and concepts to mine the fungal metabolome.

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“…SAHA (500 μM) was added in the medium of the dark septate endophytic fungus Drechslera sp., inducing the release of hexosylphytosphyngosine ( 233 ) to the culture medium [ 40 ].…”

Section: Effects Of Histone Deacetylase Modifiersmentioning

confidence: 99%

Recent Advances in Search of Bioactive Secondary Metabolites from Fungi Triggered by Chemical Epigenetic Modifiers

Xue

Hou

et al. 2023

JoF

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Genomic analysis has demonstrated that many fungi possess essential gene clusters for the production of previously unobserved secondary metabolites; however, these genes are normally reduced or silenced under most conditions. These cryptic biosynthetic gene clusters have become treasures of new bioactive secondary metabolites. The induction of these biosynthetic gene clusters under stress or special conditions can improve the titers of known compounds or the production of novel compounds. Among the inducing strategies, chemical-epigenetic regulation is considered a powerful approach, and it uses small-molecule epigenetic modifiers, which mainly act as the inhibitors of DNA methyltransferase, histone deacetylase, and histone acetyltransferase, to promote changes in the structure of DNA, histones, and proteasomes and to further activate cryptic biosynthetic gene clusters for the production of a wide variety of bioactive secondary metabolites. These epigenetic modifiers mainly include 5-azacytidine, suberoylanilide hydroxamic acid, suberoyl bishydroxamic acid, sodium butyrate, and nicotinamide. This review gives an overview on the method of chemical epigenetic modifiers to trigger silent or low-expressed biosynthetic pathways to yield bioactive natural products through external cues of fungi, mainly based on the research progress in the period from 2007 to 2022. The production of about 540 fungal secondary metabolites was found to be induced or enhanced by chemical epigenetic modifiers. Some of them exhibited significant biological activities such as cytotoxic, antimicrobial, anti-inflammatory, and antioxidant activity.

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Metabolites from the Dark Septate Endophyte Drechslera sp. Evaluation by LC/MS and Principal Component Analysis of Culture Extracts with Histone Deacetylase Inhibitors

Cited by 22 publications

References 55 publications

A Survey of Culturable Fungal Endophytes From Festuca rubra subsp. pruinosa, a Grass From Marine Cliffs, Reveals a Core Microbiome

A Survey of Culturable Fungal Endophytes From Festuca rubra subsp. pruinosa, a Grass From Marine Cliffs, Reveals a Core Microbiome

On top of biosynthetic gene clusters: How epigenetic machinery influences secondary metabolism in fungi

Recent Advances in Search of Bioactive Secondary Metabolites from Fungi Triggered by Chemical Epigenetic Modifiers

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