Metabolites characterization of timosaponin AIII in vivo and in vitro by using liquid chromatography-mass spectrometry

Sun, Yu; Liu, Li‐Yin; Peng, Ying; Liu, Bingjie; Lin, Dongju; Li, Lingzhi; Song, Shao‐Jiang

doi:10.1016/j.jchromb.2015.06.015

Cited by 16 publications

(8 citation statements)

References 22 publications

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“…The ZSF‐related direct metabolites of prototype components are produced through multiple phase I and II metabolic reactions, including demethylation, hydroxylation, oxidation, sulfation, glucuronidation, methylation and de‐caffeoyl reaction. In addition, our results indicated that timosaponin AIII underwent F‐ring cleavage and isotype reactions in rats (Sun et al, ). The proposed metabolic profile of major alkaloids, steroidal saponins and PhGs‐related metabolites in rats are shown in Fig.…”

Section: Discussionsupporting

confidence: 54%

“…The metabolites M30 and M31 at retention times of 15.58 and 16.64 min exhibited molecular ions at m / z 433.3359 and 433.3326. The same fragment ion of M30 and M31 at m / z 271 (C 19 H 27 O) suggested that the oxidation sites were not at the E‐ring and F‐ring (Sun et al, ). The above observations provided evidence that M30 and M31 should be monooxidation metabolites of sarsasapogenin.…”

Section: Investigations and Resultsmentioning

confidence: 95%

“…In the MS/MS spectra, the [M] − ion showed fragment ions at [M − Glu] − m / z 417. As for the retention time, M31 was tentatively identified as (20 R ,25 S )‐timosaponin AI (Sun et al, ). M32 showed the same formula as timosaponin A, and exhibited the same molecular formula of C 33 H 55 O 8 ([M] − m / z 579.3929) and the same fragment ion at m / z 417.…”

Section: Investigations and Resultsmentioning

confidence: 99%

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Identification of the constituents and metabolites in rats after oral administration of Zi Shen Formula by UPLC‐Q‐TOF/MS combined pattern recognition analysis

Zheng

Zhang

Geng

et al. 2017

Biomedical Chromatography

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An ultra-high-performance liquid chromatography mass spectrometry method was established to detect and identify the chemical constituents of Zi Shen Formula (ZSF) and its metabolites in serum, urine and feces, after oral administration to rats. A total of 68 compounds were characterized in ZSF extracts. In vivo, 38 prototype components and 32 metabolites of ZSF were tentatively identified in rat serum, urine and feces. Seven metabolic pathways including demethylation, hydroxylation, oxidation, sulfation, glucuronidation, methylation and de-caffeoyl were proposed to be involved in the generation of these metabolites. It was found that glucuronidation, methylation and demethylation were the major metabolic processes of alkaloids, while demethylation, methylation, sulfation and de-caffeoyl were the major metabolic pathways of phenylethanoid glycosides. The main metabolic pathways of steroidal saponins were oxidation and isotype reactions. These findings are significant for our understanding of the metabolism of ZSF. The proposed metabolic pathways of bioactive components might be crucial for further studies of the mechanisms of action and pharmacokinetic evaluations of ZSF.

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Section: Discussionsupporting

confidence: 54%

Section: Investigations and Resultsmentioning

confidence: 95%

Section: Investigations and Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Identification of the constituents and metabolites in rats after oral administration of Zi Shen Formula by UPLC‐Q‐TOF/MS combined pattern recognition analysis

Zheng

Zhang

Geng

et al. 2017

Biomedical Chromatography

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“…Frozen fecal samples were extracted, in duplicate, using a modified protocol adapted published protocols [ 39 ]. Bat fecal samples were thawed on ice.…”

Section: Methodsmentioning

confidence: 99%

Comparative metabolomics of aging in a long-lived bat: Insights into the physiology of extreme longevity

et al. 2018

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Vespertilionid bats (Mammalia: Order Chiroptera) live 3–10 times longer than other mammals of an equivalent body size. At present, nothing is known of how bat fecal metabolic profiles shift with age in any taxa. This study established the feasibility of using a non-invasive, fecal metabolomics approach to examine age-related differences in the fecal metabolome of young and elderly adult big brown bats (Eptesicus fuscus) as an initial investigation into using metabolomics for age determination. Samples were collected from captive, known-aged big brown bats (Eptesicus fuscus) from 1 to over 14 years of age: these two ages represent age groups separated by approximately 75% of the known natural lifespan of this taxon. Results showed 41 metabolites differentiated young (n = 22) and elderly (n = 6) Eptesicus. Significant differences in metabolites between young and elderly bats were associated with tryptophan metabolism and incomplete protein digestion. Results support further exploration of the physiological mechanisms bats employ to achieve exceptional longevity.

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“…Sarsasapogenin (SG) and its proto structures are the major aglycones of steroidal saponins extracted from A. asphodeloides. Meanwhile, SG is considered to be the end metabolite of these saponins such as timosaponin B II and timosaponin A III [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of sarsasapogenin in rat plasma using liquid chromatography-tandem mass spectrometry

Yang

Liu

Jing

et al. 2016

Journal of Chromatography B

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Metabolites characterization of timosaponin AIII in vivo and in vitro by using liquid chromatography-mass spectrometry

Cited by 16 publications

References 22 publications

Identification of the constituents and metabolites in rats after oral administration of Zi Shen Formula by UPLC‐Q‐TOF/MS combined pattern recognition analysis

Identification of the constituents and metabolites in rats after oral administration of Zi Shen Formula by UPLC‐Q‐TOF/MS combined pattern recognition analysis

Comparative metabolomics of aging in a long-lived bat: Insights into the physiology of extreme longevity

Quantitative determination of sarsasapogenin in rat plasma using liquid chromatography-tandem mass spectrometry

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