Quantitative determination of sarsasapogenin in rat plasma using liquid chromatography-tandem mass spectrometry

Yang, Bo; Liu, Zhirui; Jing, Hu; Lai, Xiaodan; Xia, Peiyuan

doi:10.1016/j.jchromb.2016.04.020

Cited by 12 publications

(13 citation statements)

References 17 publications

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“…As it is present in several medicinal plants [5, 7], these constitute the matrix most usually used in extraction processes for further analysis. However, other matrices can be involved too, including cosmeceutical/pharmaceutical herbal formulations, plant cell cultures, and also rat plasma samples [86–89]. However, it is important to highlight the difficulty in detecting this type of sapogenins in biological fluids due to their low concentration and lack of a chromophore in the molecule [86].…”

Section: Methodsmentioning

confidence: 99%

“…However, other matrices can be involved too, including cosmeceutical/pharmaceutical herbal formulations, plant cell cultures, and also rat plasma samples [86–89]. However, it is important to highlight the difficulty in detecting this type of sapogenins in biological fluids due to their low concentration and lack of a chromophore in the molecule [86]. At the moment, to the best of our knowledge, there are still no validated analytical methods for the detection of diosgenin in human biological samples.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, a focused microwave-assisted extraction (MAE) followed by acid hydrolysis was developed by Kaufmann et al [92] to extract diosgenin from fenugreek (Trigonella foenum graecum) leaves and roots. The preparation of plasma samples for further analysis can be achieved through a single-step procedure of protein precipitation (PPT) [86]. …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Jesus

Martins

Gallardo

et al. 2016

Journal of Analytical Methods in Chemistry

195

125

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Diosgenin, a steroidal sapogenin, occurs abundantly in plants such as Dioscorea alata, Smilax China, and Trigonella foenum graecum. This bioactive phytochemical not only is used as an important starting material for the preparation of several steroidal drugs in the pharmaceutical industry, but has revealed also high potential and interest in the treatment of various types of disorders such as cancer, hypercholesterolemia, inflammation, and several types of infections. Due to its pharmacological and industrial importance, several extraction and analytical procedures have been developed and applied over the years to isolate, detect, and quantify diosgenin, not only in its natural sources and pharmaceutical compositions, but also in animal matrices for pharmacodynamic, pharmacokinetic, and toxicological studies. Within these, HPLC technique coupled to different detectors is the most commonly analytical procedure described for this compound. However, other alternative methods were also published. Thus, the present review aims to provide collective information on the most recent pharmacological data on diosgenin and on the most relevant analytical techniques used to isolate, detect, and quantify this compound as well.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Jesus

Martins

Gallardo

et al. 2016

Journal of Analytical Methods in Chemistry

195

125

View full text Add to dashboard Cite

show abstract

“…Anemarrhenae rhizome, the rhizome of Anemarrhena asphodeloides Bunge ( Chinese Pharmacopoeia edition), has been extensively used for the clinical treatment of febrile diseases, diabetes, senile dementia and cancer (Fu, Li, Xue, Jiang, & Huang, ; Takeda et al, ; Yang, Liu, Hu, Lai, & Xia, ). Pharmacological studies have confirmed a panel of bioactivities such as anti‐inflammation, anti‐platelet aggregation, anti‐diabetic, anti‐depression, anti‐tumour and inhibitory effects on β‐amyloid (Khang, Phuong, & Ma, ; Zhang et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…A. asphodeloides mainly contains steroidal saponins, including timosaponin B‐II and timosaponin A‐III (Zhang et al, ). Sarsasapogenin is supposed to be the terminal metabolite of these saponins (Yang et al, ) and examined to have anti‐oxidative, anti‐depressant and other neuroprotective properties (Feng, Zhao, Song, Liang, & Liu, ; Ma, Zhang, Sugahara, Sagara, & Kodama, ). A variety of studies have emphasised the structural modification of sarsasapogenin and synthesised a range of derivatives exhibiting biological effects such as anti‐tumour activity, antidepressant activity, anxiolytic‐like effect and Aβ lowering effect (Gu, Fang, Liu, & Gu, ; Guo et al, ; Ma et al, ; Sy et al, ; Wang et al, ; Yin et al, ).…”

Section: Introductionmentioning

confidence: 99%

A validated UPLC–MS/MS method for quantitative determination of a potent neuroprotective agent Sarsasapogenin‐AA13 in rat plasma: Application to pharmacokinetic studies

Pei

Zhao

et al. 2020

Biomedical Chromatography

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Sarsasapogenin‐AA13(AA13), a sarsasapogenin derivative, exhibited good neuroprotective and anti‐inflammatory activities in vitro and therapeutic effects on learning and memory dysfunction in amyloid‐β‐injected mice. A sensitive UPLC–MS/MS method was developed and validated to quantitatively determine AA13 in rat plasma and was further applied to evaluate the pharmacokinetic behaviour of AA13 in rats that were administered AA13 intravenously and orally. This method was validated to exhibit excellent linearity in the concentration range of 1–1000 ng/mL. The lower limit of quantification was 1 ng/mL for AA13 in rat plasma. Intra‐day accuracy for AA13 was in the range of 90–114%, and inter‐day accuracy was in the range of 97–103 %. The relative standard deviation of intra‐day and inter‐day assay was less than 15%. After a single oral administration of AA13 at the dose of 25 mg/kg, Cmax of AA13 was 1266.4 ± 316.1 ng/mL. AUC0–48 h was 6928.5 ± 1990.1 h·ng/mL, and t1/2 was 10.2 ± 0.8 h. Under intravenous administration of AA13 at a dosage of 250 μg/kg, AUC0–48 h was 785.7 ± 103.3 h⋅ng/mL, and t1/2 was 20.8 ± 7.2 h. Based on the results, oral bioavailability (F %) of AA13 in rats at 25 mg/kg was 8.82 %.

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Synthesis and Structure Activity Relationships of Novel Dehydroepiandrosterone Derivatives as Potent Neuroprotective Agents and Nitric Oxide Production Inhibitors

Gan

Jiang

et al. 2019

ChemistrySelect

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In this study, a series of dehydroepiandrosterone (DHEA) derivatives were synthesized by linking DHEA with active fragments such as carbamate, phthalimide and indoleacetic acid. In addition, the in vitro neuroprotective effects, via the oxidative stress mechanism, and nitric oxide (NO) production inhibitory activities of each compound were tested. The preliminary structure‐activity relationships of them indicated that DHEA with carbamate structure had a certain neuroprotective and anti‐inflammatory effect such as 3β‐(Cyclohexylamine‐1‐carboxylate)‐5‐androsten‐17‐one (A2) and 3β‐(3‐Chlorobenzylamine‐1‐carboxylate)‐5‐androsten‐17‐one (A6). And the ester derivatives with indoleacetic acid like 17β–(Heteroauxin‐1‐carboxylate)‐androst‐4‐en‐3‐one (A12) also exhibited both significant neuroprotective activity and NO inhibitory activity. According to these results, compound A6 is considered as a potential agent for the treatment of Alzheimer′s disease (AD) and deserve for further investigations.

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Quantitative determination of sarsasapogenin in rat plasma using liquid chromatography-tandem mass spectrometry

Cited by 12 publications

References 17 publications

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

Diosgenin: Recent Highlights on Pharmacology and Analytical Methodology

A validated UPLC–MS/MS method for quantitative determination of a potent neuroprotective agent Sarsasapogenin‐AA13 in rat plasma: Application to pharmacokinetic studies

Synthesis and Structure Activity Relationships of Novel Dehydroepiandrosterone Derivatives as Potent Neuroprotective Agents and Nitric Oxide Production Inhibitors

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