Metabolite Profiling of Triterpene Glycosides of the Far Eastern Sea Cucumber Eupentacta fraudatrix and Their Distribution in Various Body Components Using LC-ESI QTOF-MS

Popov, Roman S.; Ivanchina, Natalia V.; Silchenko, Alexandra S.; Avilov, Sergey A.; Kalinin, Vladimir I.; Dolmatov, Igor Yu.; Stonik, Valentin A.; Dmitrenok, Pavel S.

doi:10.3390/md15100302

Cited by 19 publications

(53 citation statements)

References 39 publications

(71 reference statements)

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“…For instances, holothurinosides A/A 1 ( m / z 1303) and E/E 1 ( m / z 1287) were produced by the vacuolar cells, while the other congeners generated by the neurosecretory-like cells. Recently, Popov and co-workers also investigated the distribution of saponin congeners in various organs of sea cucumber Eupentacta fraudatrix by LC-ESI QTOF-MS and stated the same metabolite profile for the whole body extract and the other individual analysed parts [ 71 ]. However, they reported the maximal content of the vast majority of detected compounds in the body wall as compared to other studied body components of sea cucumber.…”

Section: Resultsmentioning

confidence: 99%

Distribution of Saponins in the Sea Cucumber Holothuria lessoni; the Body Wall Versus the Viscera, and Their Biological Activities

2018

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Sea cucumbers are an important ingredient of traditional folk medicine in many Asian countries, which are well-known for their medicinal, nutraceutical, and food values due to producing an impressive range of distinctive natural bioactive compounds. Triterpene glycosides are the most abundant and prime secondary metabolites reported in this species. They possess numerous biological activities ranging from anti-tumour, wound healing, hypolipidemia, pain relieving, the improvement of nonalcoholic fatty livers, anti-hyperuricemia, the induction of bone marrow hematopoiesis, anti-hypertension, and cosmetics and anti-ageing properties. This study was designed to purify and elucidate the structure of saponin contents of the body wall of sea cucumber Holothuria lessoni and to compare the distribution of saponins of the body wall with that of the viscera. The body wall was extracted with 70% ethanol, and purified by a liquid-liquid partition chromatography, followed by isobutanol extraction. A high-performance centrifugal partition chromatography (HPCPC) was conducted on the saponin-enriched mixture to obtain saponins with a high purity. The resultant purified saponins were analyzed using MALDI-MS/MS and ESI-MS/MS. The integrated and hyphenated MS and HPCPC analyses revealed the presence of 89 saponin congeners, including 35 new and 54 known saponins, in the body wall in which the majority of glycosides are of the holostane type. As a result, and in conjunction with existing literature, the structure of four novel acetylated saponins, namely lessoniosides H, I, J, and K were characterized. The identified triterpene glycosides showed potent antifungal activities against tested fungi, but had no antibacterial effects on the bacterium Staphylococcus aureus. The presence of a wide range of saponins with potential applications is promising for cosmeceutical, medicinal, and pharmaceutical products to improve human health.

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Section: Resultsmentioning

confidence: 99%

Distribution of Saponins in the Sea Cucumber Holothuria lessoni; the Body Wall Versus the Viscera, and Their Biological Activities

2018

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“…716 The proles of triterpenoid glycosides in ve different body components (respiratory trees, body walls, gonad tubules, guts, and aquapharyngeal bulbs) of the sea cucumber Eupentacta fraudatrix were found to be qualitatively similar but with some variability in minor compounds. 717 Transcriptome sequence data for three brittle star species (Ophionotus victoriae, Amphiura liformis, Ophiopsila aranea) identied a number of neuropeptide precursor proteins. 718…”

Section: Echinodermsmentioning

confidence: 99%

Marine natural products

et al. 2019

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“…Thus, our data along with previously obtained results demonstrate the presence of asterosaponins in all starfish body components. The fact that toxic triterpene glycosides, which are considered to be part of chemical defense system in sea cucumbers, were also found in all body components of sea cucumbers [20,21,22] may support the notion of the protective function of asterosaponins.…”

Section: Resultsmentioning

confidence: 88%

The Distribution of Asterosaponins, Polyhydroxysteroids and Related Glycosides in Different Body Components of the Far Eastern Starfish Lethasterias fusca

et al. 2019

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Glycoconjugated and other polar steroids of starfish have unique chemical structures and show a broad spectrum of biological activities. However, their biological functions remain not well established. Possible biological roles of these metabolites might be indicated by the studies on their distribution in the organism–producer. In order to investigate the localization of polar steroids in body components of the Far Eastern starfish Lethasterias fusca, chemical constituents of body walls, gonads, stomach, pyloric caeca, and coelomic fluid were studied by nanoflow liquid chromatography/mass spectrometry with captive spray ionization (nLC/CSI–QTOF–MS). It has been shown that the levels of polar steroids in the studied body components are qualitatively and quantitatively different. Generally, the obtained data confirmed earlier made assumptions about the digestive function of polyhydroxysteroids and protective role of asterosaponins. The highest level of polar steroids was found in the stomach. Asterosaponins were found in all body components, the main portion of free polyhydroxysteroids and related glycosides were located in the pyloric caeca. In addition, a great inter-individual variability was found in the content of most polar steroids, which may be associated with the peculiarities in their individual physiologic status.

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Metabolite Profiling of Triterpene Glycosides of the Far Eastern Sea Cucumber Eupentacta fraudatrix and Their Distribution in Various Body Components Using LC-ESI QTOF-MS

Cited by 19 publications

References 39 publications

Distribution of Saponins in the Sea Cucumber Holothuria lessoni; the Body Wall Versus the Viscera, and Their Biological Activities

Distribution of Saponins in the Sea Cucumber Holothuria lessoni; the Body Wall Versus the Viscera, and Their Biological Activities

Marine natural products

The Distribution of Asterosaponins, Polyhydroxysteroids and Related Glycosides in Different Body Components of the Far Eastern Starfish Lethasterias fusca

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