Metabolite profiling of the oilseed crop Ricinus communis during early seed imbibition reveals a specific metabolic signature in response to temperature

Ribeiro, Paulo R.; Willems, Leo A.J.; Mudde, Eva; Fernandez, Luzimar Gonzaga; Castro, Renato Delmondez de; Ligterink, Wilco; Hilhorst, H.W.M.

doi:10.1016/j.indcrop.2015.01.067

Cited by 35 publications

(23 citation statements)

References 24 publications

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“…Water can also stimulate the respiratory activity, mobilization of reserves for growth resumption of the embryonic axis and reduction in the concentration of inhibitors present in the seed (Ribeiro et al, 2015;Weitbrecht;Muller;Leubner-Metzger, 2011). Therefore, increasing the germination percentage and GSI this study indicate that treatment of pre-soaking in distilled water, allows the weakening micropliar endosperm, giving less resistance to expansion and elongation of the radicle.…”

Section: Souza R R De Et Almentioning

confidence: 67%

Optimization of jenipapo in vitro seed germination process

et al. 2016

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The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA 3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA 3 ; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of ½ MS + 15 g L -1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.Index terms: Genipa americana L.; in vitro culture; ligth quality; Rubiaceae; stratification in the dark. RESUMOA germinação in vitro de sementes é uma alternativa eficaz para obtenção de explantes com rapidez e qualidade sanitária. No entanto, sementes de jenipapeiro apresentam germinação lenta e desuniforme. Para tanto, fatores internos e externos às sementes que interferem diretamente no processo, devem ser identificados, visando à adaptação de melhores técnicas para obtenção de mudas. Neste sentido, objetivou-se otimizar a germinação in vitro de sementes de Genipa americana L. por meio da avaliação de diferentes fatores (qualidade de luz, tratamento com GA 3 , pré-embebição em água destilada, diferentes composições no meio de cultivo e estratificação no escuro). Verificou-se que a germinação das sementes foi indiferente a luz, no entanto, os melhores resultadados foram obtidos sob condições de escuro contínuo; Não houve efeito da aplicação de GA 3 exógeno; A pré-embebição em água destilada durante 48h contribui para obtenção de melhores taxas de germinação; E a redução de sais do meio MS, bem como o pré-tratamento de estratificação no escuro maximizam o potencial germinativo das sementes. Portanto, as condições ótimas para germinação in vitro de sementes de G. americana L. requer a pré-embebição em água destilada por 48 horas e a inoculação em meio de cultivo constituído de ½ MS + 15 g L-1 de sacarose, com estratificação no escuro por 16 dias, seguida pela transferência a salas de crescimento com iluminação fornecida por lâmpadas fluorescentes branca.Termos para indexação: Genipa americana L.; cultivo in vitro; qualidade de luz; Rubiaceae; estratif...

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Section: Souza R R De Et Almentioning

confidence: 67%

Optimization of jenipapo in vitro seed germination process

et al. 2016

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“…Data processing and compound identification were performed as described by Ribeiro et al (2014) and Ribeiro et al (2015). Raw data were processed by ChromaTOF software 2.0 (Leco Instruments) followed by alignment between chromatograms using the MetAlign software (Lommen, 2009).…”

Section: Gc-ms Data Processing and Compound Identificationmentioning

confidence: 99%

Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compounds

Bittencourt

Ribeiro

Franco

et al. 2015

Food Research International

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110

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The production of propolis by honeybees results from a selective collection of exudates from various plant species and present many potentialities in the pharmaceutical industry. The objective of this study was to investigate the chemical profile of Brazilian propolis, as well as their in vitro antioxidant and antibacterial activities. Gas chromatography-mass spectrometry was applied for chemical profiling of propolis extracts. Total phenolic compounds were quantified by Folin-Ciocalteu and antioxidant properties were assessed by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Antibacterial activity was assessed against Staphylococcus aureus, Bacillus subtilis, and Micrococcus luteus. Correlation and multivariate statistical analysis were used to identify potential bioactive compounds in the extracts. Twenty-nine metabolites were identified along with 34 other metabolites that were classified into the following classes: triterpenoids (12), acetyltriterpenoids (3), sesquiterpenes (6), steroids (4), and hydrocarbons (9). The antioxidant capacity (IC) ranged from 21.50 to 78.77μg/mL, whereas the content of total phenolic compounds ranged from 31.88 to 204.30mg GAE/g of dry weight. Total phenolic compounds and methyl retinoate showed a positive correlation with the antioxidant capacity, whereas tetradecanal, γ-palmitolactone and ethyl hydrocinnamate showed a negative correlation. Different sets of metabolites are shown to correlate with the antibacterial activity of the extracts, which is largely dependent on the type of microorganism. This innovative approach allowed us to identify likely bioactive compounds in the extracts, although the mechanism(s) underlying antibacterial activity encompass a complex trait, which might involve synergistic effects.

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“…Metabolites, which are responsible for antioxidant activity, could dramatically differ throughout the plant growing stages. The variations in growth might influence the germination of the plant and its quality (Mediani et al, 2012a;Ribeiro et al, 2015). The differences in the levels of secondary metabolites of some plants, harvested at different growth stages, have been recognized.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1 H NMR-based metabolomics

Mediani

Abas

Khatib

et al. 2015

Industrial Crops and Products

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a b s t r a c tSeveral studies have suggested that plants are potential sources of bioactive compounds. Phyllanthus is a plant genus that has been used in traditional medicine due to its phytomedicinal metabolites content. The variation between two Phyllanthus species (P. niruri and P. urinaria) was studied using proton nuclear magnetic resonance ( 1 H NMR) combined with multivariate data analysis (MVDA). The total phenolic content (TPC), DPPH radical scavenging activity and ␣-glucosidase inhibitory activity of the Phyllanthus species were also evaluated and correlated with their phytochemical constituents at different growth stages (8, 10 and 12 weeks) using partial least square regression (PLS). Principal component analysis (PCA) and PLS indicated separation between the two species based on the identified metabolites and the screened bioactivities. A comparison of the two species indicated that P. urinaria was separated from P. niruri due to its larger quantity of fatty and amino acids, choline, phyllanthin and sucrose. However, P. niruri contained higher quantities of hypophyllanthin and phenolic compounds. The loading column plot, which was used to compare the P. niruri at different growth stages, indicated that the eight-week-old plant contained a higher amount of fatty acids, amino acids (leucine and alanine), phyllanthin and choline. The dominant substances in the P. niruri at 10 weeks of growth by PC1 were identified as hypophyllanthin, malic acid, sucrose, and identified phenolics. The 12 week sample was differentiated by its higher sugars contents as well as malic acid and leucine. The harvested samples of both Phyllanthus species at ten weeks of age exhibited significant bioactivities with the highest content and number of metabolites.

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Metabolite profiling of the oilseed crop Ricinus communis during early seed imbibition reveals a specific metabolic signature in response to temperature

Cited by 35 publications

References 24 publications

Optimization of jenipapo in vitro seed germination process

Optimization of jenipapo in vitro seed germination process

Metabolite profiling, antioxidant and antibacterial activities of Brazilian propolis: Use of correlation and multivariate analyses to identify potential bioactive compounds

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1 H NMR-based metabolomics

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