Metabolite Profiling by UPLC-MSE, NMR, and Antioxidant Properties of Amazonian Fruits: Mamey Apple (Mammea Americana), Camapu (Physalis Angulata), and Uxi (Endopleura Uchi)

Abstract

The metabolite profiling associated with the antioxidant potential of Amazonian fruits represents an important step to the bioactive compound′s characterization due to the large biodiversity in this region. The comprehensive bioactive compounds profile and antioxidant capacities of mamey apple (Mammea americana), camapu (Physalis angulata), and uxi (Endopleura uchi) was determined for the first time. Bioactive compounds were characterized by ultra-performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-MSE) in aqueous and ethanolic extracts. Globally, a total of 293 metabolites were tentatively identified in mamey apple, campau, and uxi extracts. The main classes of compounds in the three species were terpenoids (61), phenolic acids (58), and flavonoids (53). Ethanolic extracts of fruits showed higher antioxidant activity and total ion abundance of bioactive compounds than aqueous. Uxi had the highest values of phenolic content (701.84 mg GAE/100 g), ABTS (1602.7 μmol Trolox g−1), and ORAC (15.04 μmol Trolox g−1). Mamey apple had the highest results for DPPH (1168.42 μmol TE g−1) and FRAP (1381.13 μmol FSE g−1). Nuclear magnetic resonance (NMR) spectroscopy results showed that sugars and lipids were the substances with the highest amounts in mamey apple and camapu. Data referring to chemical characteristics and antioxidant capacity of these fruits can contribute to their economic exploitation.

The Structure-Based Virtual Screening for Natural Compounds that Bind with the Activating Receptors of Natural Killer Cells

“…It exhibits anti-inflammatory and antioxidant activities. 83,84 Shikimic acid complex of platinum (II) is active against Leukemia (L1210 and P388) and B16 Melanoma cell lines. 85 The shikimic acid-based synthesis of zeylenone is widely used as a preparation for chemotherapy in cancer patients.…”

Benefits and Pitfalls of HPLC Coupled to Diode-Array, Charged Aerosol, and Coulometric Detections: Effect of Detection on Screening of Bioactive Compounds in Apples

“…Each compound that can be oxidized on the detector electrodes provides a response that represents additional information concerning substances that are potential antioxidants even if present only at low levels. For in vitro spectrophotometric evaluation of antioxidant activity, also use of ABTS, DPPH, FRAP, and ORAC tests are commonly accepted [ 43 , 44 , 45 ]. The major components with strong antioxidant activity can be easily identified by chromatographic analysis of plant extract before and after treatment with reactive components of the above-named tests [ 43 ].…”

“…For in vitro spectrophotometric evaluation of antioxidant activity, also use of ABTS, DPPH, FRAP, and ORAC tests are commonly accepted [ 43 , 44 , 45 ]. The major components with strong antioxidant activity can be easily identified by chromatographic analysis of plant extract before and after treatment with reactive components of the above-named tests [ 43 ]. For additional nutritional characterization of samples and structural characterization of isolated compounds nuclear magnetic resonance can be employed [ 43 , 44 , 45 , 46 ].…”

Analytical Technology in Nutrition Analysis

“…This Special Issue has received remarkably positive feedback, with many contributions submitted by numerous geographically diverse scientists, resulting in a collection of 10 publications, including two exhaustive review articles [9][10][11][12][13][14][15][16][17][18]. Among the contributing authors, authors can be found from Asia (China, India, and Uzbekistan), Europe (Spain), South America (Brazil and Chile) and North America (Mexico).…”

“…Among the contributing authors, authors can be found from Asia (China, India, and Uzbekistan), Europe (Spain), South America (Brazil and Chile) and North America (Mexico). The published articles include findings related to the comprehensive bioactive compound profile and antioxidant capacities of mamey apple (Mammea americana), camapu (Physalis angulata), and uxi (Endopleura uchi) that can contribute to their economic exploitation [9].…”