Metabolite identification by mass spectrometry: forty years of evolution

Wright, Pat

doi:10.3109/00498254.2011.564332

Cited by 26 publications

(11 citation statements)

References 99 publications

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“…In the last few years, several mixtures of phenolics have been analyzed with advanced LC-MS/MS equipment [17,18,19,20,21] including various publications dealing with the on-line HPLC-MS detection of flavonoids, phenolic acids and alkaloids in citrus fruits [12,13,14,15,22,23]. The advantage of HPLC-MS is its sensitivity and selectivity, and the ability to use tandem mass spectrometry for the observation of aglycones and diagnostic fragments [17,18]. As a continuation of our studies on antioxidant activity and phenolic composition of Chilean fruits [24,25], in the present work we have analyzed for the first time peels and edible pulps from three citrus varieties (pica, sutil and genova), highly consumed in northern Chile, for phenolic compounds using HPLC-UV-MS and compared their vitamin C content and antioxidant capacities.…”

Section: Introductionmentioning

confidence: 99%

HPLC-UV-MS Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile

et al. 2014

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Peels and edible pulp from three species of citrus including Citrus aurantifolia (varieties pica and sutil) and Citrus x lemon var. Genova widely cultivated and consumed in Northern Chile (I and II region) were analyzed for phenolic compounds and antioxidant activity for the first time. A high performance electrospray ionization mass spectrometry (HPLC-UV-ESI-MS) method was developed for the rapid identification of phenolics in extracts from peels and juices of all species. Several flavonoids including one kaempferol-O-hexoside (peak 16) and one hesperidin derivative (peak 22) three quercetin derivatives (peaks 4, 19 and 36), five isorhamnetin derivatives (peaks 5, 23, 24, 26 and 29) four luteolin derivatives (peaks 14, 25, 27 and 40), seven apigenin derivatives (peaks 2, 3, 12, 20, 34, 35 and 39), seven diosmetin derivatives (peaks 7-9, 17, 21, 31 and 37), three chrysoeriol derivatives (peaks 10, 18 and 30), and four eryodictiol derivatives (peaks 6, 13, 15 and 38) were identified in negative and positive mode using full scan mass measurements and MS n fragmentations. Ascorbic acid content was higher in the pulps of OPEN ACCESSMolecules 2014, 19 17401 the varieties Genova and Sutil (60.13 ± 1.28 and 56.53 ± 1.06 mg ascorbic acid per g dry weight, respectively) while total phenolic content was higher in Pica peels followed by Sutil peels (34.59 ± 0.81 and 25.58 ± 1.02 mg/g GAE dry weight, respectively). The antioxidant capacity was also higher for Pica peels (10.34 ± 1.23 µg/mL in the DPPH assay and 120.63 ± 2.45 µM trolox equivalents/g dry weight in the FRAP assay). The antioxidant features together with the high polyphenolic contents can support at least in part, the usage of the peel extracts as nutraceutical supplements, especially to be used as anti-ageing products.

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Section: Introductionmentioning

confidence: 99%

HPLC-UV-MS Profiles of Phenolic Compounds and Antioxidant Activity of Fruits from Three Citrus Species Consumed in Northern Chile

et al. 2014

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“…Polyphenols are some of principal compounds related to the benefits of fruits, vegetables, wine and plant tea consumed in the diet, due to their antioxidant properties. Plant phenolics are also useful as chemotaxonomic markers [1,2]. The polyphenols are recognized for their nutritional value, since they may help reduce the risk of chronic disease and have a positive effect on health [2,3].…”

Section: Introductionmentioning

confidence: 99%

Phenolic Profiling of the South American “Baylahuen” Tea (Haplopappus spp., Asteraceae) by HPLC-DAD-ESI-MS

2015

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Abstract:The aerial parts of several Haplopappus species (Asteraceae), known under the common name "baylahuen", are used as herbal teas in Chile and Argentina. In Chile, "baylahuen" comprises H. multifolius, H. taeda, H. baylahuen and H. rigidus. Little is known about the chemical identity of the infusion constituents in spite of widespread consumption. The aim of the present work was the characterization of phenolics occurring in the infusions and methanol extracts of "baylahuen" by HPLC-DAD-ESI-MS. A simple HPLC-DAD-ESI-MS method was developed for the fast identification and differentiation of Haplopappus spp. used as a tea source, based on the phenolics from the tea and methanol extracts. Some 27 phenolics were tentatively identified in the infusions and methanol extract, including 10 caffeoyl quinic and feruloyl quinic acid derivatives and 17 flavonoids. The HPLC patterns of the Haplopappus tea and methanol extract allow a clear differentiation at the species level. The occurrence of hydroxycinnamic acid derivatives and flavonoids can explain the reputed nutraceutical and health beneficial properties of this herbal tea.

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“…It has been reported that the response in ESI-MS differed by factor 25 for two oxidative isomeric metabolites with same chemical formula [43] or that no signal in contrary to parent drug has been observed for metabolite in positive ionization ESI. Detection of metabolite was in this case possible only in negative ionization mode [26]. However, with the commonly and easily used UV detection, the metabolites have often the same chromophore as the parent drug (but not always [40], hence giving similar response.…”

Section: Direct Quantificationmentioning

confidence: 99%

“…A short overview of analytical strategies for identification of metabolites will be provided. More information regarding metabolite identification can be found in following review articles [7,[23][24][25][26][27]. The selection of suitable LC-MS instrumentation is needed for qualitative evaluation of metabolites.…”

Section: Qualitative Evaluation Of Metabolitesmentioning

confidence: 99%