Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities

Wu, Huifeng; Liu, Jialin; Lu, Zhen; Xu, Lanlan; Ji, Chenglong; Wang, Qing; Zhao, Jianmin

doi:10.1016/j.fsi.2017.02.042

Cited by 15 publications

(4 citation statements)

References 21 publications

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“…Specifically, we show an increase of succinate, fumarate and malate concentrations in shrimp from these three origins and an increase in citrate and pyruvate concentrations in shrimp from EC and NNC, respectively, with increasing temperature. Usually, the accumulation of TCA metabolites suggests a disruption of the TCA cycle and a consequent shift from aerobic to anaerobic metabolism, as previously shown for example in the saltwater clam Laternula elliptica exposed to acute warming and in the olive flounder Paralichthys olivaceus, exposed to low salinity (Clark et al, 2017;Wu et al, 2017). In the olive flounder, the accumulation of lactate under low salinity exposure further supports the shift to anaerobic metabolism.…”

Section: Discussionmentioning

confidence: 54%

“…Recently, the use of metabolomics analyses, i.e. the identification and quantification of targeted or all metabolites of a biological system (Bundy et al, 2009), has increased in studies investigating the responses of organisms to environmental changes (Williams et al, 2009;Mayor et al, 2015;Aru et al, 2017;Wu et al, 2017;Li et al, 2020a;Li et al, 2020b), and specifically to global change drivers such as OW, OA and ocean deoxygenation, in isolation or combined (e.g. Ellis et al, 2014;Wei et al, 2015;Zhang et al, 2017;Huo et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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All roads lead to Rome: inter-origin variation in metabolomics reprogramming of the northern shrimp exposed to global changes leads to a comparable physiological status

et al. 2023

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Impacts of global ocean changes on species have historically been investigated at the whole-organism level. However, acquiring an in-depth understanding of the organisms’ cellular metabolic responses is paramount to better define their sensitivity to environmental challenges. This is particularly relevant for species that experience highly different environmental conditions across their distribution range as local acclimatization or adaptation can influence their responses to rapid global ocean changes. We aimed at shedding light on the cellular mechanisms underpinning the sensitivity to combined ocean warming (OW) and acidification (OA) in the northern shrimp Pandalus borealis, from four different geographic origins defined by distinctive environmental regimes in the northwest Atlantic: i.e. St. Lawrence Estuary (SLE), Eastern Scotian Shelf (ESS), Esquiman Channel (EC) and Northeast Newfoundland Coast (NNC). We characterized targeted metabolomics profiles of the muscle of shrimp exposed to three temperatures (2, 6 or 10°C) and two pH levels (7.75 or 7.40). Overall, shrimp metabolomics profiles were modulated by a significant interaction between temperature, pH and origin. Temperature drove most of the metabolomics reprogramming, confirming that P. borealis is more sensitive to OW than OA. Inter-origin differences in metabolomics profiles were also observed, with temperature*pH interactions impacting only shrimp from SLE and ESS, pH affecting only shrimp from SLE and temperature impacting shrimp from all origins. Temperature influenced metabolomics pathways related to the tricarboxylic acid cycle (TCA) and amino acid metabolism, resulting mainly in an accumulation of TCA intermediates and tyrosine. Temperature*pH and pH in isolation only affected amino acid metabolism, leading to amino acids accumulation under low pH. However, the ratio of ATP : ADP remained constant across conditions in shrimp from all origins suggesting that their energetic status is not affected by OW and OA. Still, the accumulation of TCA intermediates and tyrosine suggests the possible enhancement of immune responses under future OW and OA conditions. Our findings suggest that shrimp from SLE are more sensitive at the molecular level, compared to others, to future complex environmental conditions. This underlines the importance of investigating intraspecific variation in mechanisms of responses to combined drivers when trying to define species’ sensitivity to global ocean changes.

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Section: Discussionmentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

All roads lead to Rome: inter-origin variation in metabolomics reprogramming of the northern shrimp exposed to global changes leads to a comparable physiological status

et al. 2023

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“…Salinity, an important environmental factor, can influence the physiological and metabolic activity of marine fish [1,2]. Euryhaline fish can adapt to a wide range of salinity and have a superior ability for osmoregulation under chronic and rapid osmotic stress [3].…”

Section: Introductionmentioning

confidence: 99%

RNA Sequencing (RNA-Seq) Analysis Reveals Liver Lipid Metabolism Divergent Adaptive Response to Low- and High-Salinity Stress in Spotted Scat (Scatophagus argus)

Chen

Cai

Tian

et al. 2023

Animals

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Spotted scat (Scatophagus argus) can tolerate a wide range of salinity fluctuations. It is a good model for studying environmental salinity adaptation. Lipid metabolism plays an important role in salinity adaptation in fish. To elucidate the mechanism of lipid metabolism in the osmoregulation, the liver transcriptome was analyzed after 22 d culture with a salinity of 5 ppt (Low-salinity group: LS), 25 ppt (Control group: Ctrl), and 35 ppt (High-salinity group: HS) water by using RNA sequencing (RNA-seq) in spotted scat. RNA-seq analysis showed that 1276 and 2768 differentially expressed genes (DEGs) were identified in the LS vs. Ctrl and HS vs. Ctrl, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the pathways of steroid hormone biosynthesis, steroid biosynthesis, glycerophospholipid metabolism, glycerolipid metabolism, and lipid metabolism were significantly enriched in the LS vs. Ctrl. The genes of steroid biosynthesis (sqle, dhcr7, and cyp51a1), steroid hormone biosynthesis (ugt2a1, ugt2a2, ugt2b20, and ugt2b31), and glycerophospholipid metabolism (cept1, pla2g4a, and ptdss2) were significantly down-regulated in the LS vs. Ctrl. The pathways related to lipid metabolisms, such as fatty acid metabolism, fatty acid biosynthesis, peroxisome proliferator-activated receptor (PPAR) signaling pathway, adipocytokine signaling pathway, fatty acid degradation, and unsaturated fatty acid biosynthesis, were significantly enriched in the HS vs. Ctrl. The genes of unsaturated fatty acid biosynthesis (scd1, hacd3, fads2, pecr, and elovl1) and adipocytokine signaling pathway (g6pc1, socs1, socs3, adipor2, pck1, and pparα) were significantly up-regulated in the HS vs. Ctrl. These results suggest that the difference in liver lipid metabolism is important to adapt to low- and high-salinity stress in spotted scat, which clarifies the molecular regulatory mechanisms of salinity adaptation in euryhaline fish.

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“…Saline-alkali stress higher or lower than this range will affect the growth and survival of fish ( 3 ). Although many studies have examined the effects of saline-alkali stress on the osmotic regulation ( 4 – 6 ), changes in organizational structure ( 5 ), antioxidant system ( 7 – 9 ), immune system ( 10 ), energy metabolism ( 11 ), growth and survival in freshwater fish species, it still remains to lack a thorough grasp for sensing mechanisms of freshwater fish under environment salinity stress and pathogens. Pattern recognition receptors (PRRs) are known to play an essential role in sensing intracellular and extracellular products of various pathogens ( 12 ).…”

Section: Introductionmentioning

confidence: 99%

Effects and Molecular Regulation Mechanisms of Salinity Stress on the Health and Disease Resistance of Grass Carp

Fang

Yang

et al. 2022

Front. Immunol.

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Though some freshwater fish have been successfully cultivated in saline-alkali water, the survival rates of freshwater fish are greatly affected by different saline-alkali conditions. The mechanisms of immune adaptation or immunosuppression of freshwater fish under different saline-alkali stress remain unclear. Here, grass carp were exposed to 3‰ and 6‰ salinity for 30 days. It was observed that salinity treatments had no obvious effects on survival rates, but significantly increased the percent of unhealthy fish. Salinity treatments also increased the susceptibility of grass carp against Flavobacterium columnare infection. The fatality rate (16.67%) of grass carp treated with 6‰ salinity was much lower than that treated with 3‰ salinity (40%). In the absence of infection, higher numbers of immune-related DEGs and signaling pathways were enriched in 6‰ salinity-treated asymptomatic fish than in 3‰ salinity-treated asymptomatic fish. Furthermore different from salinity-treated symptomatic fish, more DEGs involved in the upstream sensors of NOD-like receptor signaling pathway, such as NLRs, were induced in the gills of 6‰ salinity-treated asymptomatic fish. However in the case of F. columnare infection, more immune-related signaling pathways were impaired by salinity treatments. Among them, only NOD-like receptor signaling pathway was significantly enriched at early (1 and/or 2 dpi) and late (7 dpi) time points of infection both for 3‰ salinity-treated and 6‰ salinity-treated fish. Besides the innate immune responses, the adaptive immune responses such as the production of Ig levels were impaired by salinity treatments in the grass carp infected with F. columnare. The present study also characterized two novel NLRs regulated by salinity stress could inhibit bacterial proliferation and improve the survival rate of infected cells. Collectively, the present study provides the insights into the possible mechanisms why the percent of unhealthy fish in the absence of infection and mortality of grass carp in the case of F. columnare infection were much lower in the 6‰ salinity-treated grass carp than in 3‰ salinity-treated grass carp, and also offers a number of potential markers for sensing both environmental salinity stress and pathogen.

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Metabolite and gene expression responses in juvenile flounder Paralichthys olivaceus exposed to reduced salinities

Cited by 15 publications

References 21 publications

All roads lead to Rome: inter-origin variation in metabolomics reprogramming of the northern shrimp exposed to global changes leads to a comparable physiological status

All roads lead to Rome: inter-origin variation in metabolomics reprogramming of the northern shrimp exposed to global changes leads to a comparable physiological status

RNA Sequencing (RNA-Seq) Analysis Reveals Liver Lipid Metabolism Divergent Adaptive Response to Low- and High-Salinity Stress in Spotted Scat (Scatophagus argus)

Effects and Molecular Regulation Mechanisms of Salinity Stress on the Health and Disease Resistance of Grass Carp

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