Metabolite analysis of Clostridium acetobutylicum: Fermentation in a microbial fuel cell

Finch, Amethist S.; Mackie, Timothy D.; Sund, Christian; Sumner, James J.

doi:10.1016/j.biortech.2010.06.149

Cited by 69 publications

(28 citation statements)

References 14 publications

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“…Isolates obtained from Swine wastewater includes Corynebacterium specie, Bacillus specie, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Streptococcus faecalis. Potential bioelectricity generating isolates used in previous studies includes Clostridium species [14,15], Geobacter sulfureducens [16], Shewanella japonica [17], Shewanella putrefaciens [10], Klebsiella specie [18], Corynebacterium species [19], Enterobacter cloacae [20], Lactococcus lactis [21] and Bacillus megaterium [22]. The bioelectrogens isolated from this experiments have also been reported as possible electrogenic bacteria that can drive the generation of electricity using the technology of MFC as reported in studies carried out by [19].…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Generation of Bioelectricity and Treatment of Swine Wastewater in a Microbial Fuel Cell

Egbadon

Akujobi

Nweke

et al. 2016

ILNS

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This study aimed at the simultaneous treatment of wastewater obtained from swine and generation of bioenergy in form of electricity from the energy stored in the organic component of the wastewater. The Open circuit voltage, current, power density and microbiological and physicochemical parameters were monitored. An initial Open circuit voltage of 516mV, Current of 0.29mA, and Power density of 32.74mW/m2were recorded, which increased to give maximum Open Circuit Voltages of 836mV, Current of 0.49mA, and Power density of 88.45mW/m2. The results revealed that The Chemical Oxygen Demand (COD), Biochemical Oxygen Demand (BOD), Organic carbon, Total Soluble solids (TSS), Ammonia, Ammonium and Ammonium-Nitrogen all showed percentage decrease of 85.92%, 51.74%, 78.16%, 98.87%, 55.87%, 55.79% and 55.90% respectively while parameters such as Total Dissolved Solids (TDS), Nitrate, Nitrate-Nitrogen, Phosphates, Phosphorus and Orthophosphates however increased after treatment to give a percentage increase of -273.60%, -131.65%, -134.85%, -168.77%, -159.26%, and -157.03% respectively. Bacteria isolates identified at the biofilms on the anode wereCorynebacteriumspecie, Bacillusspecie, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosaandStreptococcus faecalis. The results from this study further exacerbate the Bioelectricity production as well as wastewater treatment potentials of the Microbial Fuel Cell technology.

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Section: Discussionmentioning

confidence: 99%

Simultaneous Generation of Bioelectricity and Treatment of Swine Wastewater in a Microbial Fuel Cell

Egbadon

Akujobi

Nweke

et al. 2016

ILNS

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“…Decrease in pH as well as methane production lowers the ability of electricity generating microorganisms. All in all, it can be concluded from the microbial data that the glucose load was too high for fresh water sediment to efficiently generate electricity (Finch et al, 2011).…”

Section: Microbial Community Analysis In the Fuel Cell Systemmentioning

confidence: 99%

Analysis of Microbial Communities in Aquatic Sediment Microbial Fuel Cells Injected with Glucose

Kim¹,

Ekpeghere²,

Kim³

et al. 2012

The Korean Journal of Microbiology

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The purpose of this research was to optimize electric current production of sediment microbial fuel cells by injecting glucose and to investigate its impact on microbial communities involved. It was shown that injection of proper concentration of glucose could increase electric current generated from sediment microbial fuel cells. When 1,000 mg/L of glucose, as opposed to higher concentrations, was injected, electric current increased up to 3 times. This increase is mainly attributed to the mutual relationship between fermenting bacteria and exoelectrogenic bacteria. Here the organic acids generated by fermenting bacteria could be utilized by exoelectrogenic bacteria, removing feedback inhibition caused by the organic acids. When glucose was injected, the population of Clostridium increased as to ferment injected glucose. Glucose fermentation can have either a positive or negative effect on electric current generation. When exoelectrogenic bacteria may readily utilize the end-product, electric current could increase. However, when the end-product was not readily removed, then detrimental chemical reactions (pH decrease, methane generation, organic acids accumulation) occurred: exoelctrogenic bacteria population declined and non-microbial fuel cell related microorganisms prospered. By injecting a proper concentration of glucose, a mutual relationship between fermenting bacteria, such as Clostridium, and exoelectrogenic bacteria, such as Geobacter, should be fulfilled in order to increase electricity production in mixed cultures of microorganisms collected from the aquatic sediments.

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“…The cultures were spiked with a final concentration of 30 µg·mL −1 of chloramphenicol every 48 h to maintain the plasmids. Samples for metabolite analysis were taken every 24 h for 144 h. Metabolite samples were clarified via filtration through a 0.2 µm PES syringe filter (Corning) and then stored at −20 • C. HPLC analysis of samples for metabolite concentrations was performed on an Agilent 1200, as previously described [6,25]. Samples were injected into the HPLC system, eluted isocratically, and quantified using an external calibration curve of pure known components as standards.…”

Section: Growth and Metabolite Studiesmentioning

confidence: 99%

Hydrogen-Cycling during Solventogenesis in Clostridium acetobutylicum American Type Culture Collection (ATCC) 824 Requires the [NiFe]-Hydrogenase for Energy Conservation

et al. 2018

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Clostridium acetobutylicum has traditionally been used for production of acetone, butanol, and ethanol (ABE). Butanol is a commodity chemical due in part to its suitability as a biofuel; however, the current yield of this product from biological systems is not economically feasible as an alternative fuel source. Understanding solvent phase physiology, solvent tolerance, and their genetic underpinning is key for future strain optimization of the bacterium. This study shows the importance of a [NiFe]-hydrogenase in solvent phase physiology. C. acetobutylicum genes ca_c0810 and ca_c0811, annotated as a HypF and HypD maturation factor, were found to be required for [NiFe]-hydrogenase activity. They were shown to be part of a polycistronic operon with other hyp genes. Hydrogenase activity assays of the ∆hypF/hypD mutant showed an almost complete inactivation of the [NiFe]-hydrogenase. Metabolic studies comparing ∆hypF/hypD and wild type (WT) strains in planktonic and sessile conditions indicated the hydrogenase was important for solvent phase metabolism. For the mutant, reabsorption of acetate and butyrate was inhibited during solventogenesis in planktonic cultures, and less ABE was produced. During sessile growth, the ∆hypF/hypD mutant had higher initial acetone: butanol ratios, which is consistent with the inability to obtain reduced cofactors via H 2 uptake. In sessile conditions, the ∆hypF/hypD mutant was inhibited in early solventogenesis, but it appeared to remodel its metabolism and produced mainly butanol in late solventogenesis without the uptake of acids. Energy filtered transmission electron microscopy (EFTEM) mapped Pd(II) reduction via [NiFe]-hydrogenase induced H 2 oxidation at the extracelluar side of the membrane on WT cells. A decrease of Pd(0) deposits on ∆hypF/hypD comparatively to WT indicates that the [NiFe]-hydrogenase contributed to the Pd(II) reduction. Calculations of reaction potentials during acidogenesis and solventogenesis predict the [NiFe]-hydrogenase can couple NAD + reduction with membrane transport of electrons. Extracellular oxidation of H 2 combined with the potential for electron transport across the membrane indicate that the [NiFe}-hydrogenase contributes to proton motive force maintenance via hydrogen cycling.

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Metabolite analysis of Clostridium acetobutylicum: Fermentation in a microbial fuel cell

Cited by 69 publications

References 14 publications

Simultaneous Generation of Bioelectricity and Treatment of Swine Wastewater in a Microbial Fuel Cell

Simultaneous Generation of Bioelectricity and Treatment of Swine Wastewater in a Microbial Fuel Cell

Analysis of Microbial Communities in Aquatic Sediment Microbial Fuel Cells Injected with Glucose

Hydrogen-Cycling during Solventogenesis in Clostridium acetobutylicum American Type Culture Collection (ATCC) 824 Requires the [NiFe]-Hydrogenase for Energy Conservation

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